Literature DB >> 30940534

Development of a multiplex-PCR to simultaneously detect acquired linezolid resistance genes cfr, optrA and poxtA in enterococci of clinical origin.

Jennifer K Bender1, Carola Fleige2, Ingo Klare2, Guido Werner2.   

Abstract

Linezolid-resistant enterococcus spp. are increasingly recognized by diagnostic laboratories. Resistance can be mediated by the expression of cfr, optrA or poxtA. We developed a multiplex-PCR to simultaneously detect all three genes. The PCR is suitable for microbiological diagnostics in order to restrict further spread of resistances in enterococci.
Copyright © 2019 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Enterococcus spp.; Linezolid resistance; Multiplex-PCR; cfr; optrA; poxtA

Mesh：

Substances：

Year:  2019        PMID： 30940534     DOI： 10.1016/j.mimet.2019.03.025

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  8 in total

1.  Validating a screening agar for linezolid-resistant enterococci.

Authors:  Guido Werner; Carola Fleige; Ingo Klare; Robert E Weber; Jennifer K Bender
Journal:  BMC Infect Dis       Date:  2019-12-23       Impact factor: 3.090

Review 2.  Interplay between ESKAPE Pathogens and Immunity in Skin Infections: An Overview of the Major Determinants of Virulence and Antibiotic Resistance.

Authors:  Gustavo Henrique Rodrigues Vale de Macedo; Gabrielle Damasceno Evangelista Costa; Elane Rodrigues Oliveira; Glauciane Viera Damasceno; Juliana Silva Pereira Mendonça; Lucas Dos Santos Silva; Vitor Lopes Chagas; José Manuel Noguera Bazán; Amanda Silva Dos Santos Aliança; Rita de Cássia Mendonça de Miranda; Adrielle Zagmignan; Andrea de Souza Monteiro; Luís Cláudio Nascimento da Silva
Journal:  Pathogens       Date:  2021-02-02

3.  Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in Enterococcus Faecalis and Enterococcus Faecium Isolated from Food-Producing Animals and Meat in Korea.

Authors:  Eiseul Kim; So-Won Shin; Hyo-Sun Kwak; Min-Hyeok Cha; Seung-Min Yang; Yoon-Soo Gwak; Gun-Jo Woo; Hae-Yeong Kim
Journal:  Int J Mol Sci       Date:  2021-10-20       Impact factor: 5.923

4.  Linezolid-Resistant Enterococcus spp. Isolates from Foods of Animal Origin-The Genetic Basis of Acquired Resistance.

Authors:  Urszula Zarzecka; Arkadiusz Józef Zakrzewski; Wioleta Chajęcka-Wierzchowska; Anna Zadernowska
Journal:  Foods       Date:  2022-03-28

5.  Evidence of Linezolid Resistance and Virulence Factors in Enterococcus spp. Isolates from Wild and Domestic Ruminants, Italy.

Authors:  Camilla Smoglica; Alberto Vergara; Simone Angelucci; Anna Rita Festino; Antonio Antonucci; Fulvio Marsilio; Cristina Esmeralda Di Francesco
Journal:  Antibiotics (Basel)       Date:  2022-02-10

6.  Mobile Oxazolidinone Resistance Genes in Gram-Positive and Gram-Negative Bacteria.

Authors:  Stefan Schwarz; Wanjiang Zhang; Xiang-Dang Du; Henrike Krüger; Andrea T Feßler; Shizhen Ma; Yao Zhu; Congming Wu; Jianzhong Shen; Yang Wang
Journal:  Clin Microbiol Rev       Date:  2021-06-02       Impact factor: 50.129

7.  Comparative genomics of global optrA-carrying Enterococcus faecalis uncovers a common chromosomal hotspot for optrA acquisition within a diversity of core and accessory genomes.

Authors:  Ana R Freitas; Ana P Tedim; Carla Novais; Val F Lanza; Luísa Peixe
Journal:  Microb Genom       Date:  2020-03-09

8.  Evaluation of CHROMagar™ LIN-R for the Screening of Linezolid Resistant Staphylococci from Positive Blood Cultures and Nasal Swab Screening Samples.

Authors:  Delphine Girlich; Liliana Mihaila; Vincent Cattoir; Frédéric Laurent; Christine Begasse; Florence David; Carole-Ann Metro; Laurent Dortet
Journal:  Antibiotics (Basel)       Date:  2022-02-25
  8 in total

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