Kimberly Morio1, Emma L Thayer2, Amber M Bates3, Kim A Brogden4. 1. Department of Endodontics, College of Dentistry, The University of Iowa, Iowa City, Iowa. 2. Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, Iowa. 3. Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin. 4. Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, Iowa; Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, Iowa. Electronic address: kim-brogden@uiowa.edu.
Abstract
INTRODUCTION: The successful treatment of infected or inflamed endodontic tissues requires chemomechanical debridement of the canal spaces and proper sealing of the coronal and apical canal openings. Only a few methods are available to further disinfect areas or initiate regeneration of local tissues. In this study, we assessed the ability of 255-nm and 405-nm light-emitting diode (LED) treatment to kill planktonic cultures of Enterococcus faecalis and induce the production of cellular biomarkers related to endodontic tissue regeneration. METHODS: We determined the antimicrobial effects of 255-nm and 405-nm LED treatment on E. faecalis and the effects of 255-nm and 405-nm LED treatment on the production of osteoinductive, angiogenic, proliferative, and proinflammatory biomarkers from human embryonic palatal mesenchyme (HEPM) cells and gingival fibroblasts. RESULTS: We showed that 255-nm LED but not 405-nm LED treatment killed E. faecalis; the 255-nm LED and sodium hypochlorite more efficiently killed E. faecalis; neither 255-nm nor 405-nm LED treatment affected the viability of HEPM cells and gingival fibroblasts; and 255-nm LED treatment, alone or in combination with 405-nm LED treatment, of HEPM cells and gingival fibroblasts induced the production of biomarkers related to endodontic tissue regeneration. CONCLUSIONS: The results of this study suggest a new treatment modality using short periods of 255-nm LED treatment as an adjunct to chemomechanical debridement for the disinfection of inflamed sites and the production of biomarkers related to endodontic tissue regeneration.
INTRODUCTION: The successful treatment of infected or inflamed endodontic tissues requires chemomechanical debridement of the canal spaces and proper sealing of the coronal and apical canal openings. Only a few methods are available to further disinfect areas or initiate regeneration of local tissues. In this study, we assessed the ability of 255-nm and 405-nm light-emitting diode (LED) treatment to kill planktonic cultures of Enterococcus faecalis and induce the production of cellular biomarkers related to endodontic tissue regeneration. METHODS: We determined the antimicrobial effects of 255-nm and 405-nm LED treatment on E. faecalis and the effects of 255-nm and 405-nm LED treatment on the production of osteoinductive, angiogenic, proliferative, and proinflammatory biomarkers from humanembryonic palatal mesenchyme (HEPM) cells and gingival fibroblasts. RESULTS: We showed that 255-nm LED but not 405-nm LED treatment killed E. faecalis; the 255-nm LED and sodium hypochlorite more efficiently killed E. faecalis; neither 255-nm nor 405-nm LED treatment affected the viability of HEPM cells and gingival fibroblasts; and 255-nm LED treatment, alone or in combination with 405-nm LED treatment, of HEPM cells and gingival fibroblasts induced the production of biomarkers related to endodontic tissue regeneration. CONCLUSIONS: The results of this study suggest a new treatment modality using short periods of 255-nm LED treatment as an adjunct to chemomechanical debridement for the disinfection of inflamed sites and the production of biomarkers related to endodontic tissue regeneration.
Authors: Simon J Dean; Alex Petty; Simon Swift; Jennifer J McGhee; Anant Sharma; Sunil Shah; Jennifer P Craig Journal: Clin Exp Ophthalmol Date: 2011-03 Impact factor: 4.207
Authors: Praveen R Arany; Andrew Cho; Tristan D Hunt; Gursimran Sidhu; Kyungsup Shin; Eason Hahm; George X Huang; James Weaver; Aaron Chih-Hao Chen; Bonnie L Padwa; Michael R Hamblin; Mary Helen Barcellos-Hoff; Ashok B Kulkarni; David J Mooney Journal: Sci Transl Med Date: 2014-05-28 Impact factor: 17.956