INTRODUCTION: Cigarette smoking has been associated with adverse health outcomes for mothers and children and is a major contributor to heart disease. Although cigarette smoking is known to affect the epigenome, few studies have been done in African American populations. In this study, we investigated the association between cigarette smoking and DNA methylation (DNAm) among African Americans from the Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure Study (InterGEN), and the Genetic Epidemiology Network of Arteriopathy (GENOA). METHODS: The InterGEN study aims to examine the effects of genetic and psychological factors on blood pressure among African American women and their children. Current cigarette smoking was assessed at baseline. DNAm of saliva was assessed using the 850K EPIC Illumina BeadChip for Epigenome-Wide Association analyses. A replication study was conducted among 1100 participants in the GENOA study using the same BeadChip. RESULTS: After controlling for age, body mass index, population structure and cell composition, 26 epigenome-wide significant sites (FDR q < 0.05) were identified, including the AHRR and PHF14 genes associated with atherosclerosis and lung disease, respectively. Six novel CpG sites were discovered in the InterGEN sample and replicated in the GENOA sample. Genes mapped include RARA, FSIP1, ALPP, PIK3R5, KIAA0087, and MGAT3, which were largely associated with cancer development. CONCLUSION: We observed significant epigenetic associations between smoking and disease-associated genes (e.g., cardiovascular disease, lung cancer). Six novel CpG sites were identified and replicated across saliva and blood samples.
INTRODUCTION: Cigarette smoking has been associated with adverse health outcomes for mothers and children and is a major contributor to heart disease. Although cigarette smoking is known to affect the epigenome, few studies have been done in African American populations. In this study, we investigated the association between cigarette smoking and DNA methylation (DNAm) among African Americans from the Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure Study (InterGEN), and the Genetic Epidemiology Network of Arteriopathy (GENOA). METHODS: The InterGEN study aims to examine the effects of genetic and psychological factors on blood pressure among African American women and their children. Current cigarette smoking was assessed at baseline. DNAm of saliva was assessed using the 850K EPIC Illumina BeadChip for Epigenome-Wide Association analyses. A replication study was conducted among 1100 participants in the GENOA study using the same BeadChip. RESULTS: After controlling for age, body mass index, population structure and cell composition, 26 epigenome-wide significant sites (FDR q < 0.05) were identified, including the AHRR and PHF14 genes associated with atherosclerosis and lung disease, respectively. Six novel CpG sites were discovered in the InterGEN sample and replicated in the GENOA sample. Genes mapped include RARA, FSIP1, ALPP, PIK3R5, KIAA0087, and MGAT3, which were largely associated with cancer development. CONCLUSION: We observed significant epigenetic associations between smoking and disease-associated genes (e.g., cardiovascular disease, lung cancer). Six novel CpG sites were identified and replicated across saliva and blood samples.
Entities:
Keywords:
African Americans; DNA methylation; epigenomics; obesity; smoking; women
Authors: Yan V Sun; Alicia K Smith; Karen N Conneely; Qiuzhi Chang; Weiyan Li; Alicia Lazarus; Jennifer A Smith; Lynn M Almli; Elisabeth B Binder; Torsten Klengel; Dorthie Cross; Stephen T Turner; Kerry J Ressler; Sharon L R Kardia Journal: Hum Genet Date: 2013-05-09 Impact factor: 4.132
Authors: Yunfeng Huang; Qin Hui; Douglas I Walker; Karan Uppal; Jack Goldberg; Dean P Jones; Viola Vaccarino; Yan V Sun Journal: Epigenomics Date: 2018-03-12 Impact factor: 4.778
Authors: Parisa Tehranifar; Hui-Chen Wu; Jasmine A McDonald; Farzana Jasmine; Regina M Santella; Irina Gurvich; Julie D Flom; Mary Beth Terry Journal: Epigenetics Date: 2018-02-08 Impact factor: 4.528
Authors: Minghao Sun; Petra Hillmann; Bianca T Hofmann; Jonathan R Hart; Peter K Vogt Journal: Proc Natl Acad Sci U S A Date: 2010-08-16 Impact factor: 11.205
Authors: Theresa Ryan Stueve; Wen-Qing Li; Jianxin Shi; Crystal N Marconett; Tongwu Zhang; Chenchen Yang; Daniel Mullen; Chunli Yan; William Wheeler; Xing Hua; Beiyun Zhou; Zea Borok; Neil E Caporaso; Angela C Pesatori; Jubao Duan; Ite A Laird-Offringa; Maria Teresa Landi Journal: Hum Mol Genet Date: 2017-08-01 Impact factor: 6.150
Authors: Paul R Daniels; Sharon L R Kardia; Craig L Hanis; C Andrew Brown; Richard Hutchinson; Eric Boerwinkle; Stephen T Turner Journal: Am J Med Date: 2004-05-15 Impact factor: 4.965
Authors: Roby Joehanes; Allan C Just; Riccardo E Marioni; Luke C Pilling; Lindsay M Reynolds; Pooja R Mandaviya; Weihua Guan; Tao Xu; Cathy E Elks; Stella Aslibekyan; Hortensia Moreno-Macias; Jennifer A Smith; Jennifer A Brody; Radhika Dhingra; Paul Yousefi; James S Pankow; Sonja Kunze; Sonia H Shah; Allan F McRae; Kurt Lohman; Jin Sha; Devin M Absher; Luigi Ferrucci; Wei Zhao; Ellen W Demerath; Jan Bressler; Megan L Grove; Tianxiao Huan; Chunyu Liu; Michael M Mendelson; Chen Yao; Douglas P Kiel; Annette Peters; Rui Wang-Sattler; Peter M Visscher; Naomi R Wray; John M Starr; Jingzhong Ding; Carlos J Rodriguez; Nicholas J Wareham; Marguerite R Irvin; Degui Zhi; Myrto Barrdahl; Paolo Vineis; Srikant Ambatipudi; André G Uitterlinden; Albert Hofman; Joel Schwartz; Elena Colicino; Lifang Hou; Pantel S Vokonas; Dena G Hernandez; Andrew B Singleton; Stefania Bandinelli; Stephen T Turner; Erin B Ware; Alicia K Smith; Torsten Klengel; Elisabeth B Binder; Bruce M Psaty; Kent D Taylor; Sina A Gharib; Brenton R Swenson; Liming Liang; Dawn L DeMeo; George T O'Connor; Zdenko Herceg; Kerry J Ressler; Karen N Conneely; Nona Sotoodehnia; Sharon L R Kardia; David Melzer; Andrea A Baccarelli; Joyce B J van Meurs; Isabelle Romieu; Donna K Arnett; Ken K Ong; Yongmei Liu; Melanie Waldenberger; Ian J Deary; Myriam Fornage; Daniel Levy; Stephanie J London Journal: Circ Cardiovasc Genet Date: 2016-09-20
Authors: Boghuma K Titanji; Zeyuan Wang; Junyu Chen; Qin Hui; Kaku So-Armah; Matthew Freiberg; Amy C Justice; Xu Ke; Vincent C Marconi; Yan V Sun Journal: AIDS Date: 2022-06-21 Impact factor: 4.632
Authors: Ireen Klemp; Anne Hoffmann; Luise Müller; Tobias Hagemann; Kathrin Horn; Kerstin Rohde-Zimmermann; Anke Tönjes; Joachim Thiery; Markus Löffler; Ralph Burkhardt; Yvonne Böttcher; Michael Stumvoll; Matthias Blüher; Knut Krohn; Markus Scholz; Ronny Baber; Paul W Franks; Peter Kovacs; Maria Keller Journal: Clin Transl Med Date: 2022-06
Authors: Katherine C Crocker; Arce Domingo-Relloso; Karin Haack; Amanda M Fretts; Wan-Yee Tang; Miguel Herreros; Maria Tellez-Plaza; M Daniele Fallin; Shelley A Cole; Ana Navas-Acien Journal: Int J Obes (Lond) Date: 2020-07-29 Impact factor: 5.095
Authors: Wei Zhao; Farah Ammous; Scott Ratliff; Jiaxuan Liu; Miao Yu; Thomas H Mosley; Sharon L R Kardia; Jennifer A Smith Journal: Int J Environ Res Public Health Date: 2019-08-28 Impact factor: 3.390
Authors: Chengchen Li; Zeyuan Wang; Theresa Hardy; Yunfeng Huang; Qin Hui; Cindy A Crusto; Michelle L Wright; Jacquelyn Y Taylor; Yan V Sun Journal: Int J Mol Sci Date: 2019-08-31 Impact factor: 5.923
Authors: C Christiansen; J E Castillo-Fernandez; A Domingo-Relloso; W Zhao; J S El-Sayed Moustafa; P-C Tsai; J Maddock; K Haack; S A Cole; S L R Kardia; M Molokhia; M Suderman; C Power; C Relton; A Wong; D Kuh; A Goodman; K S Small; J A Smith; M Tellez-Plaza; A Navas-Acien; G B Ploubidis; R Hardy; J T Bell Journal: Clin Epigenetics Date: 2021-02-16 Impact factor: 6.551
Authors: Ke Xu; Janitza L Montalvo-Ortiz; Xinyu Zhang; Steven M Southwick; John H Krystal; Robert H Pietrzak; Joel Gelernter Journal: Alcohol Clin Exp Res Date: 2019-08-30 Impact factor: 3.928