V Darshan1, K R Indushekar2, B G Saraf1, N Sheoran1, B Sharma1, D Sardana3,4. 1. Department of Paediatric Dentistry, Sudha Rustagi Dental College, Faridabad, Haryana, India. 2. Department of Paediatric Dentistry, Educare Institute of Dental Sciences, Chattiparamba, Malappuram, Kerala, India. 3. Department of Paediatric Dentistry, Sudha Rustagi Dental College, Faridabad, Haryana, India. divesh@connect.hu.hk. 4. Discipline of Paediatric Dentistry, Faculty of Dentistry, Prince Philip Dental Hospital, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China. divesh@connect.hu.hk.
Abstract
AIM: To compare the effectiveness of different decontamination methods on tried-in preformed metal crowns (PMCs). METHODS: Sixty unused PMCs and 90 tried-in PMCs from patients were assessed for contamination after culturing for 24 h on liquid media, solid media and differential media for identification of Streptococcus mutans, Staphylococcus aureus, and Escherichia coli. Subsequently, these PMCs were divided equally into the following six groups: autoclave (121 °C, 15 psi for 15 min), 5% sodium hypochlorite (5 min), 5% glutaraldehyde (5 min), 70% isopropyl alcohol (1 min) and normal saline (5 min). The contamination was reassessed, and the Log10 counts were compared to the pre-decontamination levels using one way ANOVA and paired t-test at a significance level of p < 0.05. RESULTS: The mean percentage reduction in colony counts was significantly more in the autoclave group compared to glutaraldehyde or sodium hypochlorite groups, glass bead, isopropyl alcohol, and normal saline in this decreasing order. CONCLUSIONS: PMCs supplied by the manufacturer were found to be sterile and can be used directly on patients. The autoclave was the best method of sterilisation, although it did not eliminate the microbes 100%; followed by 5% glutaraldehyde and 5% sodium hypochlorite.
AIM: To compare the effectiveness of different decontamination methods on tried-in preformed metal crowns (PMCs). METHODS: Sixty unused PMCs and 90 tried-in PMCs from patients were assessed for contamination after culturing for 24 h on liquid media, solid media and differential media for identification of Streptococcus mutans, Staphylococcus aureus, and Escherichia coli. Subsequently, these PMCs were divided equally into the following six groups: autoclave (121 °C, 15 psi for 15 min), 5% sodium hypochlorite (5 min), 5% glutaraldehyde (5 min), 70% isopropyl alcohol (1 min) and normal saline (5 min). The contamination was reassessed, and the Log10 counts were compared to the pre-decontamination levels using one way ANOVA and paired t-test at a significance level of p < 0.05. RESULTS: The mean percentage reduction in colony counts was significantly more in the autoclave group compared to glutaraldehyde or sodium hypochlorite groups, glass bead, isopropyl alcohol, and normal saline in this decreasing order. CONCLUSIONS: PMCs supplied by the manufacturer were found to be sterile and can be used directly on patients. The autoclave was the best method of sterilisation, although it did not eliminate the microbes 100%; followed by 5% glutaraldehyde and 5% sodium hypochlorite.
Authors: K Vinay Kumar; K S Kiran Kumar; S Supreetha; K N Raghu; Anusha Channabasappa Veerabhadrappa; S Deepthi Journal: J Int Soc Prev Community Dent Date: 2015 May-Jun