Kiril Trpkov1, Sean R Williamson2, Yuan Gao1, Petr Martinek3, Liang Cheng4, Ankur R Sangoi5, Asli Yilmaz1, Cheng Wang6, Pilar San Miguel Fraile7, Delia M Perez Montiel8, Stela Bulimbasić9, Joanna Rogala10, Ondrej Hes3. 1. University of Calgary and Calgary Laboratory Services, Calgary, AB, Canada. 2. School of Medicine, Henry Ford Health System and Wayne State University, Detroit, MI, USA. 3. Charles University and University Hospital Pilsen, Pilsen, Czech Republic. 4. Indiana University School of Medicine, Indianapolis, IN, USA. 5. El Camino Hospital, Mountain View, CA, USA. 6. QEII Health Sciences Centre and Dalhousie University, Halifax, NS, Canada. 7. Hospital Álvaro Cunqueiro, Vigo (Pontevedra), Spain. 8. Institute Nacional de Cancerologia, Mexico City, Mexico. 9. University Hospital Center, Zagreb, Croatia. 10. Regional Specialist Hospital, Wroclaw, Poland.
Abstract
AIM: To describe a group of distinct low-grade oncocytic renal tumours that demonstrate CD117 negative/cytokeratin (CK) 7-positive immunoprofile. METHODS AND RESULTS: We identified 28 such tumours from four large renal tumour archives. We performed immunohistochemistry for: CK7, CD117, PAX8, CD10, AMACR, e-cadherin, CK20, CA9, AE1/AE3, vimentin, BerEP4, MOC31, CK5/6, p63, HMB45, melan A, CD15 and FH. In 14 cases we performed array CGH, with a successful result in nine cases. Median patient age was 66 years (range 49-78 years) with a male-to-female ratio of 1:1.8. Median tumour size was 3 cm (range 1.1-13.5 cm). All were single tumours, solid and tan-brown, without a syndromic association. On microscopy, all cases showed solid and compact nested growth. There were frequent areas of oedematous stroma with loosely arranged cells. The tumour cells had oncocytic cytoplasm with uniformly round to oval nuclei, but without significant irregularities, and showed only focal perinuclear halos. Negative CD117 and positive CK7 reactivity were present in all cases (in two cases there was focal and very weak CD117 reactivity). Uniform reactivity was found for PAX8, AE1/AE3, e-cadherin, BerEP4 and MOC31. Negative stains included CA9, CK20, vimentin, CK5/6, p63, HMB45, Melan A and CD15. CD10 and AMACR were either negative or focally positive; FH was retained. On array CGH, there were frequent deletions at 19p13.3 (seven of nine), 1p36.33 (five of nine) and 19q13.11 (four of nine); disomic status was found in two of nine cases. On follow-up (mean 31.8 months, range 1-118), all patients were alive with no disease progression. CONCLUSION: Low-grade oncocytic tumours that are CD117-negative/CK7-positive demonstrate consistent and readily recognisable morphology, immunoprofile and indolent behaviour.
AIM: To describe a group of distinct low-grade oncocytic renal tumours that demonstrate CD117 negative/cytokeratin (CK) 7-positive immunoprofile. METHODS AND RESULTS: We identified 28 such tumours from four large renal tumour archives. We performed immunohistochemistry for: CK7, CD117, PAX8, CD10, AMACR, e-cadherin, CK20, CA9, AE1/AE3, vimentin, BerEP4, MOC31, CK5/6, p63, HMB45, melan A, CD15 and FH. In 14 cases we performed array CGH, with a successful result in nine cases. Median patient age was 66 years (range 49-78 years) with a male-to-female ratio of 1:1.8. Median tumour size was 3 cm (range 1.1-13.5 cm). All were single tumours, solid and tan-brown, without a syndromic association. On microscopy, all cases showed solid and compact nested growth. There were frequent areas of oedematous stroma with loosely arranged cells. The tumour cells had oncocytic cytoplasm with uniformly round to oval nuclei, but without significant irregularities, and showed only focal perinuclear halos. Negative CD117 and positive CK7 reactivity were present in all cases (in two cases there was focal and very weak CD117 reactivity). Uniform reactivity was found for PAX8, AE1/AE3, e-cadherin, BerEP4 and MOC31. Negative stains included CA9, CK20, vimentin, CK5/6, p63, HMB45, Melan A and CD15. CD10 and AMACR were either negative or focally positive; FH was retained. On array CGH, there were frequent deletions at 19p13.3 (seven of nine), 1p36.33 (five of nine) and 19q13.11 (four of nine); disomic status was found in two of nine cases. On follow-up (mean 31.8 months, range 1-118), all patients were alive with no disease progression. CONCLUSION: Low-grade oncocytic tumours that are CD117-negative/CK7-positive demonstrate consistent and readily recognisable morphology, immunoprofile and indolent behaviour.
Authors: Kiril Trpkov; Sean R Williamson; Anthony J Gill; Ondrej Hes; Adebowale J Adeniran; Abbas Agaimy; Reza Alaghehbandan; Mahul B Amin; Pedram Argani; Ying-Bei Chen; Liang Cheng; Jonathan I Epstein; John C Cheville; Eva Comperat; Isabela Werneck da Cunha; Jennifer B Gordetsky; Sounak Gupta; Huiying He; Michelle S Hirsch; Peter A Humphrey; Payal Kapur; Fumiyoshi Kojima; Jose I Lopez; Fiona Maclean; Cristina Magi-Galluzzi; Jesse K McKenney; Rohit Mehra; Santosh Menon; George J Netto; Christopher G Przybycin; Priya Rao; Qiu Rao; Victor E Reuter; Rola M Saleeb; Rajal B Shah; Steven C Smith; Satish Tickoo; Maria S Tretiakova; Lawrence True; Virginie Verkarre; Sara E Wobker; Ming Zhou Journal: Mod Pathol Date: 2021-02-01 Impact factor: 7.842
Authors: Talia L Fuchs; Fiona Maclean; John Turchini; A Cristina Vargas; Selina Bhattarai; Abbas Agaimy; Arndt Hartmann; Chia-Sui Kao; Carla Ellis; Michael Bonert; Xavier Leroy; Lakshmi P Kunju; Lauren Schwartz; Admire Matsika; Sean R Williamson; Priya Rao; Mukul Divatia; Rosa Guarch; Ferran Algaba; Marcelo L Balancin; Ming Zhou; Hemamali Samaratunga; Isabela Werneck da Cunha; Fadi Brimo; Andrew Ryan; David Clouston; Manju Aron; Marie O'Donnell; Emily Chan; Michelle S Hirsch; Holger Moch; Chun-Yin Pang; Cheuk Wah; Weihua Yin; Joanna Perry-Keene; Asli Yilmaz; Angela Chou; Adele Clarkson; Gerhard van der Westhuizen; Ella Morrison; Jonathan Zwi; Ondrej Hes; Kiril Trpkov; Anthony J Gill Journal: Mod Pathol Date: 2021-12-23 Impact factor: 7.842