RNAi-mediated protection against banana diseases and pests.

Pests and pathogens restrict the production potential of many crop plants. The losses incurred due to pests and diseases are huge threatening food security. Management strategies include use of chemical pesticides which can be detrimental to human health and environment and other physical and biological methods which have serious limitations. An alternative would be to utilize the advanced technology such as RNA interference (RNAi) to engineer disease resistance in crop plants. The phenomenon of RNAi is very well studied in organisms across genera and found to be conserved. Taking advantage of this, dsRNAs have been delivered into pests and pathogens and showed significant growth inhibition. Banana is susceptible to various groups of pathogens which results in poor yield. The proof-of-principle studies using RNAi technology have already been demonstrated in banana to develop resistance to two important groups of pathogens. Transgenic banana plants expressing small interfering RNA targeting BBTV and Fusarium pathogen have shown high level of resistance. In this review, we summarize and discuss the studies utilizing RNAi as a strategy to develop resistance to major banana diseases and encourage further research in exploiting RNAi-based resistance in other crop plants.