| Literature DB >> 30853725 |
John A Brailsford1, Jennifer L Stockdill1, Abram J Axelrod1, Michael T Peterson1, Paul A Vadola1, Eric V Johnston1, Samuel J Danishefsky1.
Abstract
The β-subunit of human thyroid stimulating hormone (hTSH) has been synthesized as a single glycoform bearing a chitobiose disaccharide at the native glycosylation site. Key to the successful completion of this synthesis was the introduction of an arginine-tagged acetamidomethyl group, which served to greatly facilitate handling of a glycopeptide fragment with poor aqueous solubility. This general solution to the challenge of working with intractable peptides is expected to find wide use in protein synthesis.Entities:
Year: 2018 PMID： 30853725 PMCID： PMC6402344 DOI： 10.1016/j.tet.2018.02.067
Source DB: PubMed Journal: Tetrahedron ISSN： 0040-4020 Impact factor: 2.457