Literature DB >> 3085333

Preliminary investigations of a correlation between electron energy loss and morphometric analyses on ultrathin cryosections from normal and neoplastic gastric tissues.

B Wolf, E Bischoff, A Schwinde.   

Abstract

Electron energy loss spectroscopy (EELS) has been used to measure the ratios of C, N, O, P and Ca in ultrathin cryosections from normal and neoplastic gastric tissues. First results show a correlation between the EELS, and morphometric data in cells from these tissues. We have found that ultrathin, freeze-dried cryosections, with an average thickness of up to 75 nm, are stable enough for EELS-analysis in a 200 KV electron microscope with an adapted Gatan-EELS-Spectrometer.

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Year:  1986        PMID： 3085333     DOI： 10.1007/bf00705344

Source DB:  PubMed          Journal:  Virchows Arch A Pathol Anat Histopathol        ISSN： 0174-7398


  11 in total

1.  An ultrastructural morphometric analysis of normal human mammary tissue and human breast cancer.

Authors:  B Wolf; J Thompson; A Schwinde
Journal:  Anticancer Res       Date:  1985 Mar-Apr       Impact factor: 2.480

2.  An ultrastructural morphometric analysis on ultrathin epon and ultrathin cryosections of normal human gastric tissue and human gastric cancer.

Authors:  B Wolf; E Müller; A Schwinde
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1985

3.  [A semiautomatic intelligent morphometer for electron microscopy images and online morphometry with the electron microscope].

Authors:  B Wolf
Journal:  Mikroskopie       Date:  1983-09

4.  [Automatic ultramicrotome drive for routine medical histology with the electron microscope].

Authors:  B Wolf; A Schwinde
Journal:  Microsc Acta       Date:  1983-05

5.  Electron spectroscopic imaging: parallel energy filtering and microanalysis in the fixed-beam electron microscope.

Authors:  F P Ottensmeyer
Journal:  J Ultrastruct Res       Date:  1984-08

6.  On the preparation of cryosections for immunocytochemistry.

Authors:  G Griffiths; A McDowall; R Back; J Dubochet
Journal:  J Ultrastruct Res       Date:  1984-10

7.  Calcium content of mitochondria and endoplasmic reticulum in liver frozen rapidly in vivo.

Authors:  A P Somlyo; M Bond; A V Somlyo
Journal:  Nature       Date:  1985 Apr 18-24       Impact factor: 49.962

8.  Stimulated formation of rER-mitochondrial complexes in liver parenchymal cells from rats surviving phalloidin poisoning.

Authors:  B Wolf; H Schimassek
Journal:  Mikroskopie       Date:  1983-04

9.  Immunochemistry on ultrathin frozen sections.

Authors:  K T Tokuyasu
Journal:  Histochem J       Date:  1980-07

Review 10.  Ultrastructure of the human breast and its disorders.

Authors:  E R Fisher
Journal:  Am J Clin Pathol       Date:  1976-08       Impact factor: 2.493
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  4 in total

Review 1.  [Modeling in biology. Structured analysis of intracellular calcium oscillations in electrically non-excitable cells].

Authors:  M Kraus; B Wolf
Journal:  Naturwissenschaften       Date:  1992-07

Review 2.  [Electron energy loss spectroscopy (EELS) as a method for the localization of antigens and other substances in cells and tissues].

Authors:  B Wolf; W G Bessler
Journal:  Naturwissenschaften       Date:  1990-03

3.  Systems analysis in cell biology: from the phenomenological description towards a computer model of the intracellular signal transduction network.

Authors:  M Kraus; P Lais; B Wolf
Journal:  Experientia       Date:  1993-03-15

Review 4.  [Importance of cell self-organization for tumor biology].

Authors:  B Wolf; M Kraus
Journal:  Naturwissenschaften       Date:  1993-08
  4 in total

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