Eman A Toraih1,2, Aya El-Wazir1,2, Mohammad H Hussein3, Moataz S Khashana4, Amgad Matter5, Manal S Fawzy6,7, Somaya Hosny2,8. 1. 1 Genetics Unit, Histology and Cell Biology Department, Faculty of Medicine, Suez Canal University (FOM/SCU), Ismailia, Egypt. 2. 2 Center of Excellence in Molecular and Cellular Medicine, FOM/SCU, Ismailia, Egypt. 3. 3 Ministry of Health and Population, Cairo, Egypt. 4. 4 Faculty of Medicine, Suez Canal University, Ismailia, Egypt. 5. 5 Department of Neurological surgery, FOM/SCU, Ismailia, Egypt. 6. 6 Department of Medical Biochemistry and Molecular Biology, FOM/SCU, Ismailia, Egypt. 7. 7 Department of Biochemistry, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia. 8. 8 Department of Histology and Cell Biology, FOM/SCU, Ismailia, Egypt.
Abstract
BACKGROUND: Long intergenic non-coding RNA, regulator of reprogramming ( LINC-ROR) is a newly identified cytoplasmic long non-coding RNA (lncRNA), which has been found to be dysregulated in different cancers. The present work aimed to quantify LINC-ROR expression profile and assess the tumor proteins p53 and caspase 3 expressions in glioblastoma tissue specimens compared to non-cancer tissues, and to correlate these expression levels with the available clinicopathological and survival data. METHODS: LINC-ROR relative expression in 57 glioblastoma cancer tissues and 10 non-cancer tissues was quantified by real-time polymerase chain reaction (qPCR). In addition, methylation-specific PCR of O-6-methylguanine-DNA methyltransferase ( MGMT) promoter and immunohistochemical expression of apoptosis related proteins: p53 and caspase 3 were performed. RESULTS: The up-regulation of LINC-ROR was encountered in 89.5% of patients. The higher expression of LINC-ROR was associated with poor disease progression-free and overall survival as well as a younger age of patients ( P=0.036). p53 protein was expressed only in glioblastoma but not in non-cancer tissues while caspase 3 was weakly expressed in most non-cancer tissues and in varying degrees in glioblastoma (24% weak, 30% moderate, and 16% strong expression). The Kaplan-Meier survival plot illustrated poor survival in glioblastoma patients with over-expressed LINC-ROR ( P=0.010) and down-regulated p53 ( P=0.002). Multivariate analysis showed that glioblastoma patients were clustered into two distinct groups based on LINC-ROR expression profile, p53 staining levels and patients' overall survival. CONCLUSIONS: LINC-ROR up-regulation may have a role in glioblastoma tumorigenesis and could be a potential prognostic marker for this fatal disease.
BACKGROUND: Long intergenic non-coding RNA, regulator of reprogramming ( LINC-ROR) is a newly identified cytoplasmic long non-coding RNA (lncRNA), which has been found to be dysregulated in different cancers. The present work aimed to quantify LINC-ROR expression profile and assess the tumor proteins p53 and caspase 3 expressions in glioblastoma tissue specimens compared to non-cancer tissues, and to correlate these expression levels with the available clinicopathological and survival data. METHODS: LINC-ROR relative expression in 57 glioblastoma cancer tissues and 10 non-cancer tissues was quantified by real-time polymerase chain reaction (qPCR). In addition, methylation-specific PCR of O-6-methylguanine-DNA methyltransferase ( MGMT) promoter and immunohistochemical expression of apoptosis related proteins: p53 and caspase 3 were performed. RESULTS: The up-regulation of LINC-ROR was encountered in 89.5% of patients. The higher expression of LINC-ROR was associated with poor disease progression-free and overall survival as well as a younger age of patients ( P=0.036). p53 protein was expressed only in glioblastoma but not in non-cancer tissues while caspase 3 was weakly expressed in most non-cancer tissues and in varying degrees in glioblastoma (24% weak, 30% moderate, and 16% strong expression). The Kaplan-Meier survival plot illustrated poor survival in glioblastoma patients with over-expressed LINC-ROR ( P=0.010) and down-regulated p53 ( P=0.002). Multivariate analysis showed that glioblastoma patients were clustered into two distinct groups based on LINC-ROR expression profile, p53 staining levels and patients' overall survival. CONCLUSIONS: LINC-ROR up-regulation may have a role in glioblastoma tumorigenesis and could be a potential prognostic marker for this fatal disease.
Authors: Aly A M Shaalan; Sara H Mokhtar; Hanadi Talal Ahmedah; Amany I Almars; Eman A Toraih; Afaf T Ibrahiem; Manal S Fawzy; Mai A Salem Journal: Biomolecules Date: 2022-04-12
Authors: Yassin Ismail; Dina M Fahmy; Maivel H Ghattas; Mai M Ahmed; Walaa Zehry; Samy M Saleh; Dina M Abo-Elmatty Journal: Front Pharmacol Date: 2022-09-07 Impact factor: 5.988