| Literature DB >> 30850184 |
Aleksandra Klimas1, Gloria Ortiz2, Steven C Boggess2, Evan W Miller3, Emilia Entcheva4.
Abstract
Combined optogenetic stimulation and optical imaging permit scalable, contact-free high-throughput probing of cellular electrophysiology and optimization of stem-cell derived excitable cells, such as neurons and muscle cells. We report a new "on-axis" configuration (combined single optical path for stimulation and for multiparameter imaging) of OptoDyCE, our all-optical platform for studying human induced pluripotent stem-cell-derived cardiomyocytes (hiPSC-CMs) and other cell types, optically driven by Channelrhodopsin2 (ChR2). This solid-state system integrates optogenetic stimulation with temporally-multiplexed simultaneous recording of membrane voltage (Vm) and intracellular calcium ([Ca2+]i) dynamics using a single photodetector. We demonstrate the capacity for combining multiple spectrally-compatible actuators and sensors, including newer high-performance near-infrared (NIR) voltage probes BeRST1 and Di-4-ANBDQBS, to record complex spatiotemporal responses of hiPSC-CMs to drugs in a high-throughput manner.Entities:
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Year: 2019 PMID： 30850184 PMCID： PMC6728218 DOI： 10.1016/j.pbiomolbio.2019.02.004
Source DB: PubMed Journal: Prog Biophys Mol Biol ISSN： 0079-6107 Impact factor: 3.667