Cristina Pires Camargo1, Renan Dias Frassei2, Daniel Imbassahy de Sá Bittencourt Camara E Silva2, Robert Zawadzki Pfann2, Luiza de Campos Moreira da Silva2, Julio Morais-Besteiro1, Rolf Gemperli3. 1. MD, Division of Plastic Surgery, Hospital das Clínicas, Laboratory of Microsurgery and Plastic Surgery (LIM-04), Medical School, Universidade de São Paulo (USP), Brazil. Intellectual and scientific content of the study, interpretation of data, statistics analysis, manuscript writing, critical revision. 2. Graduate student, Laboratory of Microsurgery and Plastic Surgery (LIM-04), Medical School, USP, Sao Paulo-SP, Brazil. Acquisition, analysis and interpretation of data; technical procedures. 3. PhD, Division of Plastic Surgery, Hospital das Clínicas, Laboratory of Microsurgery and Plastic Surgery (LIM-04), Medical School, USP, Sao Paulo-SP, Brazil. Intellectual and scientific content of the study, interpretation of data, critical revision.
Abstract
PURPOSE: To evaluate the hyaluronic acid (HA) inflammatory reaction, fibroblasts, fibrosis and duration of effect in the dorsal region of tobacco-exposed rats. METHODS: Ten Wistar rats were divided into two groups: tobacco-exposed-group (TEG;n=5) and air-control-group (CG;n=5). The TEG animals were tobacco-exposed twice a day, 30-minutes/session, during 60 days. After this period, all animals received 0.1 mL HA subcutaneous injection in the dorsal area. The volume of HA was measured immediately after HA injection and weekly using a hand-caliper in nine weeks. After this period, all the animals were euthanized, and a specimen of was collected to evaluate inflammatory cells, fibroblasts, and fibrosis by HE. RESULTS: This study showed a higher inflammatory reaction in TEG than CG: inflammatory cell-count (CG: 1.07±0.9; TEG: 8.61±0.36, p<0.001); fibroblast count (CG: 2.92±0.17; TEG: 19.14±0.62, p<0.001), and fibrosis quantification (CG: 2.0; TEG: 3.75, p<0.001). The analysis of the HA volume in nine weeks in the dorsal region did not show a difference between groups (p=0.39). CONCLUSIONS: This study suggested that the HA injection in the TEG caused an increase in inflammatory cell count, fibroblast, and fibrosis quantification when compared to the CG. There was no difference in the duration of effect of HA between the groups.
PURPOSE: To evaluate the hyaluronic acid (HA) inflammatory reaction, fibroblasts, fibrosis and duration of effect in the dorsal region of tobacco-exposed rats. METHODS: Ten Wistar rats were divided into two groups: tobacco-exposed-group (TEG;n=5) and air-control-group (CG;n=5). The TEG animals were tobacco-exposed twice a day, 30-minutes/session, during 60 days. After this period, all animals received 0.1 mL HA subcutaneous injection in the dorsal area. The volume of HA was measured immediately after HA injection and weekly using a hand-caliper in nine weeks. After this period, all the animals were euthanized, and a specimen of was collected to evaluate inflammatory cells, fibroblasts, and fibrosis by HE. RESULTS: This study showed a higher inflammatory reaction in TEG than CG: inflammatory cell-count (CG: 1.07±0.9; TEG: 8.61±0.36, p<0.001); fibroblast count (CG: 2.92±0.17; TEG: 19.14±0.62, p<0.001), and fibrosis quantification (CG: 2.0; TEG: 3.75, p<0.001). The analysis of the HA volume in nine weeks in the dorsal region did not show a difference between groups (p=0.39). CONCLUSIONS: This study suggested that the HA injection in the TEG caused an increase in inflammatory cell count, fibroblast, and fibrosis quantification when compared to the CG. There was no difference in the duration of effect of HA between the groups.
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