Maciej Nowacki1, Katarzyna Pietkun1, Marta Pokrywczyńska1, Marta Rasmus1, Karolina Warda1, Tomasz Kloskowski2, Arkadiusz Jundziłł1, Maciej Gagat1, Alina Grzanka1, Magdalena Bodnar1, Andrzej Marszałek1, Tomasz Drewa1, Rafał Czajkowski1. 1. Mr Nowacki is an MD and PhD student, Dr Jundziłł is a volunteer, Dr Pokrywczyńska and Dr Kloskowski are research fellows, and Ms Rasmus and Ms Warda are MSc students in the Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University Collegium Medicum in Bydgoszcz, PolandMr Gagat is a research fellow and Dr Grzanka is Professor and Head of the Department of Histology and Embryology, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, PolandDr Bodnar is a research fellow in the Department of Clinical Pathomorphology, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, Poland, and Dr Marszałek is Professor and head of the Department of Clinical Pathomorphology, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, PolandDepartment of Oncologic Pathology, Poznan University of Medical Sciences and Greater Poland Oncology Center, Poznan, PolandMs Pietkun is an MD and PhD student in the Chair of Regenerative Medicine, Department of Tissue Engineering and Dermatology, Sexually Transmitted Diseases and Immunodermatology, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, PolandDr Czajkowski is a Professor and Head of the Department of Dermatology, Sexually Transmitted Diseases and Immunodermatology, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, PolandDr Drewa is Professor and Head of the Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University Collegium Medicum in Bydgoszcz, Poland, and Head of the Urology and Oncological Urology Department, Nicolaus Copernicus Hospital, Toruń, Poland. 2. Mr Nowacki is an MD and PhD student, Dr Jundziłł is a volunteer, Dr Pokrywczyńska and Dr Kloskowski are research fellows, and Ms Rasmus and Ms Warda are MSc students in the Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University Collegium Medicum in Bydgoszcz, PolandMr Gagat is a research fellow and Dr Grzanka is Professor and Head of the Department of Histology and Embryology, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, PolandDr Bodnar is a research fellow in the Department of Clinical Pathomorphology, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, Poland, and Dr Marszałek is Professor and head of the Department of Clinical Pathomorphology, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, PolandDepartment of Oncologic Pathology, Poznan University of Medical Sciences and Greater Poland Oncology Center, Poznan, PolandMs Pietkun is an MD and PhD student in the Chair of Regenerative Medicine, Department of Tissue Engineering and Dermatology, Sexually Transmitted Diseases and Immunodermatology, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, PolandDr Czajkowski is a Professor and Head of the Department of Dermatology, Sexually Transmitted Diseases and Immunodermatology, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, PolandDr Drewa is Professor and Head of the Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University Collegium Medicum in Bydgoszcz, Poland, and Head of the Urology and Oncological Urology Department, Nicolaus Copernicus Hospital, Toruń, Poland tomaszkloskowski@op.pl.
Abstract
BACKGROUND: Research is scarce regarding the effectiveness of dermal fillers containing autologous stem cells. OBJECTIVES: The authors sought to determine the local and systemic effects of adipose-derived stem cells (ADSCs) as a component of dermal fillers in an animal model. METHODS: Wistar rats were injected with 1 of the following dermal fillers: ADSCs combined with hyaluronic acid (ADSC-HA), ADSCs combined with fish collagen (ADSC-COL), HA alone (CONTROL-HA), or COL alone (CONTROL-COL). Fillers were injected into the glabella, dorsum, and chest of each animal. The ADSCs were labeled with PKH26 to assess cell migration. Filling effects (FEs) were measured immediately after injection and at 1.5 months and 3 months after injection. Skin specimens were stained with hematoxylin and eosin to assess localization and persistence of ADSCs. RESULTS: Mean FEs in animals implanted with ADSCs were greater and persisted longer than those of controls. No inflammatory responses were observed in any group. Three months after injection, PKH26-positive cells comprised nearly 70% of cells at the injection site in animals treated with ADSC-HA. PKH26 fluorescence also was detected in the spleen but not in the brain, kidney, or lung. CONCLUSIONS: Stem cells have the potential to improve the aesthetic effects and longevity of dermal fillers.
BACKGROUND: Research is scarce regarding the effectiveness of dermal fillers containing autologous stem cells. OBJECTIVES: The authors sought to determine the local and systemic effects of adipose-derived stem cells (ADSCs) as a component of dermal fillers in an animal model. METHODS:Wistar rats were injected with 1 of the following dermal fillers: ADSCs combined with hyaluronic acid (ADSC-HA), ADSCs combined with fish collagen (ADSC-COL), HA alone (CONTROL-HA), or COL alone (CONTROL-COL). Fillers were injected into the glabella, dorsum, and chest of each animal. The ADSCs were labeled with PKH26 to assess cell migration. Filling effects (FEs) were measured immediately after injection and at 1.5 months and 3 months after injection. Skin specimens were stained with hematoxylin and eosin to assess localization and persistence of ADSCs. RESULTS: Mean FEs in animals implanted with ADSCs were greater and persisted longer than those of controls. No inflammatory responses were observed in any group. Three months after injection, PKH26-positive cells comprised nearly 70% of cells at the injection site in animals treated with ADSC-HA. PKH26 fluorescence also was detected in the spleen but not in the brain, kidney, or lung. CONCLUSIONS: Stem cells have the potential to improve the aesthetic effects and longevity of dermal fillers.
Authors: Cristina Pires Camargo; Renan Dias Frassei; Daniel Imbassahy de Sá Bittencourt Camara E Silva; Robert Zawadzki Pfann; Luiza de Campos Moreira da Silva; Julio Morais-Besteiro; Rolf Gemperli Journal: Acta Cir Bras Date: 2019-02-28 Impact factor: 1.388
Authors: Maciej Nowacki; Katarzyna Pietkun; Arkadiusz Jundziłł; Tomasz Kloskowski; Dariusz Grzanka; Joanna Skopinska-Wisniewska; Kinga Scibior; Maciej Gagat; Marta Pokrywczyńska; Alina Grzanka; Wojciech Zegarski; Rafał Czajkowski; Tomasz Drewa; Barbara Zegarska Journal: Biomed Res Int Date: 2016-10-10 Impact factor: 3.411
Authors: Maciej Nowacki; Tomasz Kloskowski; Katarzyna Pietkun; Maciej Zegarski; Marta Pokrywczyńska; Samy L Habib; Tomasz Drewa; Barbara Zegarska Journal: Postepy Dermatol Alergol Date: 2017-12-31 Impact factor: 1.837
Authors: Jan Adamowicz; Marta Pokrywczyńska; Jakub Tworkiewicz; Tomasz Kowalczyk; Shane V van Breda; Dominik Tyloch; Tomasz Kloskowski; Magda Bodnar; Joanna Skopinska-Wisniewska; Andrzej Marszałek; Malgorzata Frontczak-Baniewicz; Tomasz A Kowalewski; Tomasz Drewa Journal: PLoS One Date: 2016-01-14 Impact factor: 3.240
Authors: Maciej Nowacki; Łukasz Nazarewski; Tomasz Kloskowski; Dominik Tyloch; Marta Pokrywczyńska; Katarzyna Pietkun; Arkadiusz Jundziłł; Janusz Tyloch; Samy L Habib; Tomasz Drewa Journal: Arch Med Sci Date: 2016-08-25 Impact factor: 3.318
Authors: Janaína José Dos Santos Machado; Bernard Gomes Piñeiro; Isalira Peroba Ramos; Sergio Augusto Lopes de Souza; Bianca Gutfilen; Maria Helena Nicola; Paulo Roberto Cotrim de Souza; Eduardo Cruz; Regina Coeli Goldenberg Journal: Stem Cells Int Date: 2020-02-14 Impact factor: 5.443