Yonghai Lu1,2, Jinling Fang3, Li Zou3, Liang Cui4, Xu Liang5, Seng Gee Lim6, Yock-Young Dan7, Choon Nam Ong3,5. 1. Saw Swee Hock School of Public Health, National University of Singapore, Tahir Foundation Building, Level 11, 12 Science Drive 2, Singapore, 117549, Singapore. ephluyng@nus.edu.sg. 2. Institute of Nutrition and Health, Qingdao University, Qingdao, Shandong, China. ephluyng@nus.edu.sg. 3. Saw Swee Hock School of Public Health, National University of Singapore, Tahir Foundation Building, Level 11, 12 Science Drive 2, Singapore, 117549, Singapore. 4. Singapore-MIT Alliance for Research & Technology (SMART), Singapore, Singapore. 5. NUS Environment Research Institute, National University of Singapore, Singapore, Singapore. 6. Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, NUHS Tower Block, Level 11, 1E Kent Ridge Road, Singapore, 119228, Singapore. 7. Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, NUHS Tower Block, Level 11, 1E Kent Ridge Road, Singapore, 119228, Singapore. mdcdyy@nus.edu.sg.
Abstract
INTRODUCTION: Chronic hepatitis B virus (HBV) infection is the main etiologic risk factor for hepatocellular carcinoma (HCC). Early studies indicated that the increase of omega-6-derived oxylipins may be involved in the pathogenesis of HBV-related HCC, yet their changes during the distinct clinical phases of chronic HBV infection remain unclear. To fill this gap, in this study we investigated the omega-6-derived oxylipin profiles in patients with three major clinical stages of chronic HBV infection (chronic hepatitis B, liver cirrhosis, and HCC). METHODS: Eighteen omega-6-derived oxylipins were quantified in serum samples of 34 patients with chronic hepatitis B, 46 patients with HBV-related liver cirrhosis, 38 patients with HBV-related HCC, and 50 healthy controls using liquid chromatography tandem mass spectrometry. RESULTS: Seven oxylipins were found to be altered in patients with HBV-related liver diseases, including 9,10-dihydroxyoctadecenoic acid (9,10-DiHOME), 12,13-DiHOME, 14,15-dihydroxyeicosatrienoic acid (14,15-DiHETrE), 13-hydroxyoctadecadienoic acid (13-HODE), 12-hydroxyeicosatetraenoic acid (12-HETE), 11-HETE, and thromboxane B2 (TXB2). Of these, three oxylipins derived from the cytochrome P450 (CYP450) pathways including 9,10-DiHOME, 12,13-DiHOME, and 14,15-DiHETrE were found to be associated with the levels of α-fetoprotein (AFP), a tumor marker. In combination with AFP, age, and gender, a combination of these seven differential oxylipins could significantly enhance the prediction of HBV-related liver diseases, particularly for liver cirrhosis (p < 0.05). CONCLUSION: This study for the first time shows the correlations between CYP450-derived oxylipins and the progression of chronic HBV infection, and sheds a new light on the surveillance of HBV-related live diseases using oxylipins.
INTRODUCTION:Chronic hepatitis B virus (HBV) infection is the main etiologic risk factor for hepatocellular carcinoma (HCC). Early studies indicated that the increase of omega-6-derived oxylipins may be involved in the pathogenesis of HBV-related HCC, yet their changes during the distinct clinical phases of chronic HBV infection remain unclear. To fill this gap, in this study we investigated the omega-6-derived oxylipin profiles in patients with three major clinical stages of chronic HBV infection (chronic hepatitis B, liver cirrhosis, and HCC). METHODS: Eighteen omega-6-derived oxylipins were quantified in serum samples of 34 patients with chronic hepatitis B, 46 patients with HBV-related liver cirrhosis, 38 patients with HBV-related HCC, and 50 healthy controls using liquid chromatography tandem mass spectrometry. RESULTS: Seven oxylipins were found to be altered in patients with HBV-related liver diseases, including 9,10-dihydroxyoctadecenoic acid (9,10-DiHOME), 12,13-DiHOME, 14,15-dihydroxyeicosatrienoic acid (14,15-DiHETrE), 13-hydroxyoctadecadienoic acid (13-HODE), 12-hydroxyeicosatetraenoic acid (12-HETE), 11-HETE, and thromboxane B2 (TXB2). Of these, three oxylipins derived from the cytochrome P450 (CYP450) pathways including 9,10-DiHOME, 12,13-DiHOME, and 14,15-DiHETrE were found to be associated with the levels of α-fetoprotein (AFP), a tumor marker. In combination with AFP, age, and gender, a combination of these seven differential oxylipins could significantly enhance the prediction of HBV-related liver diseases, particularly for liver cirrhosis (p < 0.05). CONCLUSION: This study for the first time shows the correlations between CYP450-derived oxylipins and the progression of chronic HBV infection, and sheds a new light on the surveillance of HBV-related live diseases using oxylipins.
Authors: Ye Sun; Hiromi W L Koh; Hyungwon Choi; Woon-Puay Koh; Jian-Min Yuan; John W Newman; Jin Su; Jinling Fang; Choon Nam Ong; Rob M van Dam Journal: J Lipid Res Date: 2016-05-24 Impact factor: 5.922
Authors: Katrin Strassburg; Annemarie M L Huijbrechts; Kirsten A Kortekaas; Jan H Lindeman; Theresa L Pedersen; Adrie Dane; Ruud Berger; Arjan Brenkman; Thomas Hankemeier; John van Duynhoven; Eric Kalkhoven; John W Newman; Rob J Vreeken Journal: Anal Bioanal Chem Date: 2012-07-20 Impact factor: 4.142
Authors: Hong Y Pan; Qing Q Wu; Qiao Q Yin; Yi N Dai; Yi C Huang; Wei Zheng; Tian C Hui; Mei J Chen; Ming S Wang; Jia J Zhang; Hai J Huang; Yong X Tong Journal: ACS Omega Date: 2021-01-04