Literature DB >> 3082814

Synthesis and expression of a synthetic gene for the activated human c-Ha-ras protein.

K Miura, Y Inoue, H Nakamori, S Iwai, E Ohtsuka, M Ikehara, S Noguchi, S Nishimura.   

Abstract

A 576 base-pair DNA encoding human c-Ha-ras protein (p21) with a valine codon at position 12 has been synthesized by ligation of chemically synthesized oligodeoxyribonucleotides. The duplex was inserted into a plasmid with the E. coli tryptophan promoter and was expressed in E. coli efficiently.

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Year:  1986        PMID： 3082814

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN： 0910-5050


  23 in total

1.  Prenylation of mammalian Ras protein in Xenopus oocytes.

Authors:  R Kim; J Rine; S H Kim
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

2.  An abasic site analogue activates a c-Ha-ras gene by a point mutation at modified and adjacent positions.

Authors:  H Kamiya; M Suzuki; Y Komatsu; H Miura; K Kikuchi; T Sakaguchi; N Murata; C Masutani; F Hanaoka; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

3.  Loop domain peptides from the SOS ras-guanine nucleotide exchange protein, identified from molecular dynamics calculations, strongly inhibit ras signaling.

Authors:  Lyndon Chie; Fred K Friedman; Thomas Duncan; James M Chen; Denise Chung; Matthew Pincus
Journal:  Protein J       Date:  2004-04       Impact factor: 2.371

4.  Identification of an intracellular protein that specifically interacts with photoaffinity-labeled oncogenic p21 protein.

Authors:  G Lee; Z A Ronai; M R Pincus; P W Brandt-Rauf; R B Murphy; T M Delohery; S Nishimura; Z Yamaizumi; I B Weinstein
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

5.  Specificity of inhibition of ras-p21 signal transduction by peptides from GTPase activating protein (GAP) and the son-of sevenless (SOS) ras-specific guanine nucleotide exchange protein.

Authors:  Lyndon Chie; Denise Chung; Matthew R Pincus
Journal:  Protein J       Date:  2005-05       Impact factor: 2.371

6.  Crystal structure of an active form of RAS protein, a complex of a GTP analog and the HRAS p21 catalytic domain.

Authors:  A T Brünger; M V Milburn; L Tong; A M deVos; J Jancarik; Z Yamaizumi; S Nishimura; E Ohtsuka; S H Kim
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

7.  Identification of neurofibromin mutants that exhibit allele specificity or increased Ras affinity resulting in suppression of activated ras alleles.

Authors:  P Morcos; N Thapar; N Tusneem; D Stacey; F Tamanoi
Journal:  Mol Cell Biol       Date:  1996-05       Impact factor: 4.272

8.  A comprehensive list of chemically synthesized genes.

Authors:  G Gröger; F Ramalho-Ortigao; H Steil; H Seliger
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

9.  Complexes of p21RAS with JUN N-terminal kinase and JUN proteins.

Authors:  V Adler; M R Pincus; P W Brandt-Rauf; Z Ronai
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-07       Impact factor: 11.205

10.  Evidence that the ras oncogene-encoded p21 protein induces oocyte maturation via activation of protein kinase C.

Authors:  D L Chung; P W Brandt-Rauf; I B Weinstein; S Nishimura; Z Yamaizumi; R B Murphy; M R Pincus
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-01       Impact factor: 11.205
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