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Literature DB >> 3082322

Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase.

G C Ness, C E Sample, M Smith, L C Pendleton, D C Eichler.

Abstract

A procedure for the preparation of rat liver microsomal fractions essentially devoid of contaminating lysosomes is described. When this preparation was examined by immunoblotting with a rabbit antiserum to rat 3-hydroxy-3-methylglutaryl-CoA reductase, a single band corresponding to an Mr of 100000 was observed. No evidence was found for glycosylation of rat liver-3-hydroxy-3-methylglutaryl-CoA reductase. Native rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase differs from the purified proteolytically modified species in that it displays allosteric kinetics towards NADPH.

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Year: 1986 PMID： 3082322 PMCID： PMC1152999 DOI： 10.1042/bj2330167

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

23 in total

1. Isolation of modified liver lysosomes.

Authors: A Trouet
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

2. Subcellular fractionation of rat liver.

Authors: S Fleischer; M Kervina
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

3. Activation of rat liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase by NADPH. Effects of dietary treatments.

Authors: G C Ness; S J Eales; L C Pendleton; M Smith
Journal: J Biol Chem Date: 1985-10-15 Impact factor: 5.157

4. Identification and isolation of ovalbumin-synthesizing polysomes. I. Specific binding of 125 I-anti-ovalbumin to polysomes.

Authors: R Palacios; R D Palmiter; R T Schimke
Journal: J Biol Chem Date: 1972-04-25 Impact factor: 5.157

5. Solubilization and partial purification of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase.

Authors: R A Heller; R G Gould
Journal: Biochem Biophys Res Commun Date: 1973-02-05 Impact factor: 3.575

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. HMG CoA reductase: a negatively regulated gene with unusual promoter and 5' untranslated regions.

Authors: G A Reynolds; S K Basu; T F Osborne; D J Chin; G Gil; M S Brown; J L Goldstein; K L Luskey
Journal: Cell Date: 1984-08 Impact factor: 41.582

8. Sequence of the amino-terminal 349 residues of rabbit muscle glycogen phosphorylase including the sites of covalent and allosteric control.

Authors: A Koide; K Titani; L H Ericsson; S Kumar; H Neurath; K A Walsh
Journal: Biochemistry Date: 1978-12-26 Impact factor: 3.162

9. Purification of 3-hydroxy-3-methylglutaryl coenzyme A reductase from rat liver.

Authors: G C Ness; C D Spindler; M H Moffler
Journal: Arch Biochem Biophys Date: 1979-10-15 Impact factor: 4.013

10. Fast skeletal muscle myosin light chains 1 and 3 are produced from a single gene by a combined process of differential RNA transcription and splicing.

Authors: M Periasamy; E E Strehler; L I Garfinkel; R M Gubits; N Ruiz-Opazo; B Nadal-Ginard
Journal: J Biol Chem Date: 1984-11-10 Impact factor: 5.157

12 in total

1. In vivo regulation of human mononuclear leukocyte 3-hydroxy-3-methylglutaryl coenzyme A reductase. Decreased enzyme catalytic efficiency in familial hypercholesterolemia.

Authors: P W Stacpoole; D M Bridge; I M Alvarez; R B Goldberg; H J Harwood
Journal: J Clin Invest Date: 1987-11 Impact factor: 14.808

2. Loss of NADPH during assays of HMG-CoA reductase: implications and approaches to minimize errors.

Authors: G C Ness; L C Pendleton; A S Pendleton
Journal: Lipids Date: 1987-06 Impact factor: 1.880

3. 7-Dehydrocholesterol-dependent proteolysis of HMG-CoA reductase suppresses sterol biosynthesis in a mouse model of Smith-Lemli-Opitz/RSH syndrome.

Authors: B U Fitzky; F F Moebius; H Asaoka; H Waage-Baudet; L Xu; G Xu; N Maeda; K Kluckman; S Hiller; H Yu; A K Batta; S Shefer; T Chen; G Salen; K Sulik; R D Simoni; G C Ness; H Glossmann; S B Patel; G S Tint
Journal: J Clin Invest Date: 2001-09 Impact factor: 14.808

4. Enhancement of sterol synthesis by the monoterpene perillyl alcohol is unaffected by competitive 3-hydroxy-3-methylglutaryl-CoA reductase inhibition.

Authors: S R Cerda; J Wilkinson; S K Branch; S A Broitman
Journal: Lipids Date: 1999-06 Impact factor: 1.880

5. A discoordinate increase in the cellular amount of 3-hydroxy-3-methylglutaryl-CoA reductase results in the loss of rate-limiting control over cholesterogenesis in a tumour cell-free system.

Authors: N I Azrolan; P S Coleman
Journal: Biochem J Date: 1989-03-01 Impact factor: 3.857

6. Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs.

Authors: Jie Wei; Hye Won Kang; David E Cohen
Journal: Biochem J Date: 2009-06-26 Impact factor: 3.857

10. Regulation of HMG-CoA reductase: identification of the site phosphorylated by the AMP-activated protein kinase in vitro and in intact rat liver.

Authors: P R Clarke; D G Hardie
Journal: EMBO J Date: 1990-08 Impact factor: 11.598