Luigi Barrea1, Giuseppe Annunziata2, Giovanna Muscogiuri3, Daniela Laudisio3, Carolina Di Somma4, Maria Maisto2, Gian Carlo Tenore2, Annamaria Colao3, Silvia Savastano3. 1. Department of Clinical Medicine and Surgery, Unit of Endocrinology, Federico II University Medical School of Naples, Naples, Italy. Electronic address: luigi.barrea@unina.it. 2. Department of Pharmacy, University of Naples "Federico II", Naples, Italy. 3. Department of Clinical Medicine and Surgery, Unit of Endocrinology, Federico II University Medical School of Naples, Naples, Italy. 4. Institute for Hospitalization and Healthcare SDN, Naples, Italy.
Abstract
OBJECTIVES: Sex exerts an important influence on food preferences. The Mediterranean diet (MD) is based on the common dietary characteristics and lifestyle behaviors of the Mediterranean countries. Trimethylamine N-oxide (TMAO), a marker of gut dysbiosis linked to the development of atherosclerosis and cardiovascular risk, is mainly dependent on dietary pattern and gut microbiota metabolism. The aim of this study was to evaluate the association between levels of TMAO and adherence to the MD as a function of sex. METHODS: We enrolled 144 healthy adults, of which 67 were men. Participants were 31.55 ± 6.19 y of age and had an average body mass index of 22.84 ± 1.51 kg/m2. TMAO levels were detected using high-performance liquid chromatography-mass spectrometry analysis. PREDIMED (PREvención con DIeta MEDiterránea [Prevention with Mediterranean Diet]) questionnaire was used to assess the adherence to the MD. Dietary data were collected by a 7-d food records. Receiver operator characteristic curve analysis was performed to determine the predictive values for PREDIMED score in detecting high TMAO values. RESULTS: Compared with women, the men presented higher levels of TMAO (P < 0.001), lower adherence to the MD (P = 0.017) and higher energy intake. The men consumed a greater quantity of animal proteins, carbohydrates, and ω-3 polyunsaturated fatty acids, and less plant proteins and ω-6 polyunsaturated fatty acids than the women. At the receiver operator characteristic analyses, the lowest levels of TMAO were well predicted by a score of adherence of ≤10 in men and ≤9 in women (P < 0.001). CONCLUSIONS: A clear sex difference was observed in the apparently novel association between levels of TMAO and MD in healthy adults. Although dietary intervention trials on large series population are mandatory, sex-specific cutpoints of adherence to MD might help identify individuals at high risk for high levels of TMAO who would benefit from personalized dietary interventions.
OBJECTIVES: Sex exerts an important influence on food preferences. The Mediterranean diet (MD) is based on the common dietary characteristics and lifestyle behaviors of the Mediterranean countries. Trimethylamine N-oxide (TMAO), a marker of gut dysbiosis linked to the development of atherosclerosis and cardiovascular risk, is mainly dependent on dietary pattern and gut microbiota metabolism. The aim of this study was to evaluate the association between levels of TMAO and adherence to the MD as a function of sex. METHODS: We enrolled 144 healthy adults, of which 67 were men. Participants were 31.55 ± 6.19 y of age and had an average body mass index of 22.84 ± 1.51 kg/m2. TMAO levels were detected using high-performance liquid chromatography-mass spectrometry analysis. PREDIMED (PREvención con DIeta MEDiterránea [Prevention with Mediterranean Diet]) questionnaire was used to assess the adherence to the MD. Dietary data were collected by a 7-d food records. Receiver operator characteristic curve analysis was performed to determine the predictive values for PREDIMED score in detecting high TMAO values. RESULTS: Compared with women, the men presented higher levels of TMAO (P < 0.001), lower adherence to the MD (P = 0.017) and higher energy intake. The men consumed a greater quantity of animal proteins, carbohydrates, and ω-3 polyunsaturated fatty acids, and less plant proteins and ω-6 polyunsaturated fatty acids than the women. At the receiver operator characteristic analyses, the lowest levels of TMAO were well predicted by a score of adherence of ≤10 in men and ≤9 in women (P < 0.001). CONCLUSIONS: A clear sex difference was observed in the apparently novel association between levels of TMAO and MD in healthy adults. Although dietary intervention trials on large series population are mandatory, sex-specific cutpoints of adherence to MD might help identify individuals at high risk for high levels of TMAO who would benefit from personalized dietary interventions.
Authors: Laura E Griffin; Zora Djuric; Chris J Angiletta; Cassie M Mitchell; Mary E Baugh; Kevin P Davy; Andrew P Neilson Journal: Food Funct Date: 2019-04-02 Impact factor: 5.396
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Authors: Zhendong Mei; Guo-Chong Chen; Zheng Wang; Mykhaylo Usyk; Bing Yu; Yoshiki Vazquez Baeza; Greg Humphrey; Rodolfo Salido Benitez; Jun Li; Jessica S Williams-Nguyen; Martha L Daviglus; Lifang Hou; Jianwen Cai; Yan Zheng; Rob Knight; Robert D Burk; Eric Boerwinkle; Robert C Kaplan; Qibin Qi Journal: Am J Clin Nutr Date: 2021-06-01 Impact factor: 7.045