| Literature DB >> 30818053 |
Ángel Sánchez-Illana1, Vidhi Shah2, José David Piñeiro-Ramos1, Juliann M Di Fiore3, Guillermo Quintás4, Thomas M Raffay3, Peter M MacFarlane3, Richard J Martin3, Julia Kuligowski5.
Abstract
Oxidative stress plays an essential role in processes of signaling and damage to biomolecules during early perinatal life. Isoprostanoids and isofuranoids from the free radical-catalyzed peroxidation of polyunsaturated fatty acids (PUFAs) are widely recognized as reliable biomarkers of oxidative stress. However, their quantification is not straightforward due to high structural similarity of the compounds formed. In this work, a semiquantitative method for the analysis of adrenic acid (AdA, C22:4 n-6) non-enzymatic peroxidation products (i.e. dihomo-isoprostanes and dihomo-isofurans) was developed. The proposed ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) method was applied to the analysis of blood plasma and urine from preterm infants providing information about AdA peroxidation.Entities:
Keywords: Dihomo-isofurans; Dihomo-isoprostanes; Lipid peroxidation; Preterm infants; UPLC-MS/MS
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Year: 2019 PMID： 30818053 PMCID： PMC6800232 DOI： 10.1016/j.freeradbiomed.2019.02.024
Source DB: PubMed Journal: Free Radic Biol Med ISSN： 0891-5849 Impact factor: 7.376