Sachi Miyake1, Ayako Ogo2, Hisako Kubota3, Fusako Teramoto2, Toshihiro Hirai2. 1. Department of Doctoral Program in Health Science, Graduate School of Health Science and Technology, Kawasaki University of Medical Welfare, Kurashiki, Japan s-miyake@mw.kawasaki-m.ac.jp. 2. Department of Doctoral Program in Health Science, Graduate School of Health Science and Technology, Kawasaki University of Medical Welfare, Kurashiki, Japan. 3. Department of Digestive Surgery, Kawasaki Medical School, Kurashiki, Japan.
Abstract
BACKGROUND/AIM: Stress reactions, especially those related to surgery, cause poor convalescence of cancer patients. β-Hydroxyβ-methylbutyrate (HMB) is known to regulate excessive inflammation in the body. The objective of this work was to investigate the capacity of HMB to suppress activation of nuclear factor-kappa B (NF-ĸB) and production of interleukin-6 (IL-6) in a human esophageal squamous cell carcinoma cell line (TE-1). MATERIALS AND METHODS: Cell proliferation was measured using the water-soluble tetrazolium-1 method, while tumor necrosis factor alpha (TNFα)-induced IL-6 production was measured using an enzyme-linked immunosorbent assay (ELISA) assay. Nuclear translocation of NF-ĸB was detected by immunofluorescence staining. RESULTS: HMB did not affect cell proliferation. However, HMB suppressed the TNFα-induced increase in IL-6 production in TE-1 cells by inhibiting NF-ĸB activation. CONCLUSION: HMB did not influence TE-1 cell proliferation, but inhibited activation of NF-ĸB and IL-6 production. This result may be useful for improving excessive stress reactions during and after surgery. Copyright
BACKGROUND/AIM: Stress reactions, especially those related to surgery, cause poor convalescence of cancerpatients. β-Hydroxyβ-methylbutyrate (HMB) is known to regulate excessive inflammation in the body. The objective of this work was to investigate the capacity of HMB to suppress activation of nuclear factor-kappa B (NF-ĸB) and production of interleukin-6 (IL-6) in a humanesophageal squamous cell carcinoma cell line (TE-1). MATERIALS AND METHODS: Cell proliferation was measured using the water-soluble tetrazolium-1 method, while tumor necrosis factor alpha (TNFα)-induced IL-6 production was measured using an enzyme-linked immunosorbent assay (ELISA) assay. Nuclear translocation of NF-ĸB was detected by immunofluorescence staining. RESULTS:HMB did not affect cell proliferation. However, HMB suppressed the TNFα-induced increase in IL-6 production in TE-1 cells by inhibiting NF-ĸB activation. CONCLUSION:HMB did not influence TE-1 cell proliferation, but inhibited activation of NF-ĸB and IL-6 production. This result may be useful for improving excessive stress reactions during and after surgery. Copyright