| Literature DB >> 30797881 |
Yuliya V Zakalyukina1, Mikhail V Birykov2, Dmitrii A Lukianov3, Dmitrii I Shiriaev4, Ekaterina S Komarova5, Dmitry A Skvortsov4, Yury Kostyukevich3, Vadim N Tashlitsky4, Vladimir I Polshakov6, Eugene Nikolaev3, Petr V Sergiev7, Ilya A Osterman8.
Abstract
A novel strain of Actinomycetes was isolated from the body of an ant (Camponotus vagus Scopoli) and its genetic and morphological properties were characterized. The 16S rDNA gene sequence analysis of the isolate revealed its high phylogenetic relationship with type strains of Streptomyces violaceochromogenes NBRC 13100T. As a result of antimicrobial activity assessment, it was found that the fermentation broth of the isolated strain both inhibited the growth and induced the SOS response in E. coli BW25113 ΔtolC strain cells. Using bioassay-guided fractionation, mass spectrometric and NMR analyses we identified the active compound to be nybomycin, a previously described antibiotic. Here we report for the first time Streptomyces producer of nybomycin in association with carpenter ants and demonstrate cytotoxic activity of nybomycin against human cell lines.Entities:
Keywords: Actinobacteria; Antibacterial activity; Ants; Camponotus; Nybomycin; Reporter strain; Streptomyces
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Year: 2019 PMID： 30797881 DOI： 10.1016/j.biochi.2019.02.010
Source DB: PubMed Journal: Biochimie ISSN： 0300-9084 Impact factor: 4.079