Size effect of nano-hydroxyapatite on proliferation of odontoblast-like MDPC-23 cells.

Nano-hydroxyapatite (nano-HAP) is supposed to be a promising candidate for apatite substitute in hard tissue engineering. We aimed to investigate the effect of nano-HAP particles on the proliferation of odontoblast-like MDPC-23 cells compared with conventional hydroxyapatite (c-HAP). HAP in diameter of ~20 nm (np20), ~70 nm (np70) and ~200 nm (c-HAP) were synthesized and characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and field emission scanning electron microscopy (FESEM). Inverted microscope and MTT assay were used to detect the morphology and proliferation rate of MDPC-23 cells; TEM was used to reveal the internalization of HAP. We found that nano-HAP (np20 and np70), especially np20 expressed obvious growth-promoting effect on MDPC-23 cells compared with c-HAP, which caused the most vacuole in MDPC-23 cells. These results suggest that nano-HAP may be an optimal choice of apatite substitute for MDPC-23 cells on the aspect of cell proliferation.