Postmastectomy radiotherapy using three different techniques: a retrospective evaluation of the incidental dose distribution in the internal mammary nodes.

OBJECTIVE: To evaluate the incidental coverage dose to the internal mammary nodes (IMN) in patients treated with postmastectomy radiotherapy (PMRT) and its relationship with the treatment plan. PATIENTS AND METHODS: We retrospectively analyzed 138 patients undergoing PMRT and divided them into three groups: three-dimensional conformal radiotherapy (3D-CRT), field-in-field forward intensity-modulated radiotherapy (F-IMRT), and inverse intensity-modulated radiotherapy (I-IMRT). The IMN were contoured according to the Radiation Therapy Oncology Group consensus and not included in the planning target volume. We analyzed incidental IMN dose coverage and its relationship with the lung and heart.
RESULTS: The mean dose (Dmean) to the IMN was 32.85 Gy for all patients, and the dose delivered to the IMN showed no differences in 3D-CRT, F-IMRT, and I-IMRT (33.80, 29.65, and 32.95 Gy, respectively). In addition, 10.42%, 2.04%, and 9.76% of patients achieved ≥45 Gy with 3D-CRT, F-IMRT, and I-IMRT, respectively. No differences were evident among the three treatment plans regarding IMN dose in the first three intercostal spaces (ICS1-3). The Dmean, V20, V30, V40, and V50 of ICS2 and ICS3 were superior to those of ICS1 for all three plans. For 3D-CRT, a moderate positive correlation was evident between the Dmean to the IMN and the Dmean to the heart. For F-IMRT and I-IMRT, positive correlations were evident between the Dmean of the IMN and the Dmean and V20 of the lung.
CONCLUSION: The mean incidental dose to the IMN for IMRT (F-IMRT and I-IMRT) and 3D-CRT after modified radical mastectomy was insufficient to treat subclinical disease. A substantial dose was delivered to the IMN in some patients. Higher incidental doses to the IMN were associated with a higher heart mean dose for 3D-CRT and a higher dose to the lung for IMRT. Future prospective studies should further explore subgroups that do not require IMN irradiation.

Background

Radiotherapy (RT) after breast conserving surgery (BCS) or mastectomy can provide significant locoregional control, decrease the risk of distant metastases, and translate into significant survival benefits,1–3 especially for patients with given molecular subtypes.4–6 The American College of Surgeons Oncology Group (ACOSOG) Z0011 Phase III trial has confirmed that, for patients with clinical T1–T2 invasive breast cancer who are treated with lumpectomy and who have one or two identified sentinel lymph nodes (SLNs) containing metastases, the use of SLN dissection (SLND) alone compared with axillary lymph node dissection (ALND) does not result in inferior overall survival (OS) or disease-free survival (DFS).7,8 Furthermore, the same observations apply to patients with negative SLNs, and the National Surgical Adjuvant Breast and Bowel Project Phase III protocol B-32 (NSABP B-32) has corroborated the superiority of SLND to the ALND treatment approach in terms of OS, DFS, and regional control.9,10 In both studies, adjuvant systemic therapy was delivered to >85% of the patients, and a portion of the level I–II axilla was irradiated in patients who underwent standard opposing tangential irradiation. Therefore, although a portion of the lymph nodes (LNs) was not irradiated, a portion of the postsurgical breast patients who received adjuvant systemic therapy (chemotherapy, hormonal therapy, or targeted therapy) combined with LN incidental irradiation during RT still exhibited diminished locoregional recurrence.

The ACOSOG Z0011 and NSABP B-32 trials have provoked speculations regarding whether the incidental radiation dose to ALNs with tangential radiation therapy fields could have prevented axillary recurrences in the SNB-only arm.7,9 In addition, recent studies have evaluated the axillary incidental irradiation dose coverage.11–14 To date, whether the incidental radiation dose to the axilla is responsible for eliminating additional hidden metastases in ALNs when the breast alone is irradiated remains unclear. Along with ALNs, internal mammary nodes (IMN) are the first filter stations for the lymphatic drainage of the breast, and the unexpected control of IMN that accompanies incidental IMN irradiation has been an ongoing controversy, especially for one to three LN-positive breast cancer and high-risk, node-negative stage II breast cancer.15–18

Despite the high incidence of IMN metastases,19–21 the overall rate of clinically detectable recurrences in IMN after primary systemic breast cancer treatment is <1%, even when regional IMN are not irradiated.22,23 As with ALN, the low recurrence rates in IMN are attributable not only to patients receiving adjuvant systemic therapy but also to IMN incidental irradiation.23,24 The literature is sparse on the incidental doses received with no internal mammary LN surgical dissection, especially with newer techniques. Recently, reports on the incidental irradiation of IMN in breast cancer have examined treatment with three-dimensional conformal RT (3D-CRT).24–27 Therefore, in this study, we compared the dosimetric parameters for incidental irradiation to the IMN during chest wall RT with 3D-CRT, field-in-field forward intensity-modulated radiotherapy (F-IMRT), and inverse intensity-modulated radiotherapy (I-IMRT). In addition, we aimed to examine these dose distribution patterns and their possible clinical relevance.

Patients and methods

Patient selection and instructions

Eligibility criteria for our study were as follows. Patients with diagnoses of invasive carcinoma or ductal carcinoma in situ of the breast who received adjuvant RT at our institution between April 2013 and May 2017 were enrolled. Patients were treated with modified radical mastectomy (MRM) with SLND or ALND, and without immediate reconstruction. Also, these patients with breast cancer who had been irradiated postoperatively were included in this retrospective study. All patients were confirmed as having no clinical or pathological evidence of IMN involvement at the time of diagnosis. The institutional research ethics board of Shandong Cancer Hospital approved this study (SDTHEC201703014), and the requirement to obtain written informed consent from patients was waived due to the retrospective nature of the investigation. Also, this study was carried out in accordance with the Declaration of Helsinki principles.

Delineation of target volume and organs at risk

The clinical target volumes (CTVs) of the chest wall and supraclavicular fossa (SCF), heart, ipsilateral lung (IPSL), and spinal cord were delineated, and the IMN were not included in the CTV. The planning target volume (PTV) margin was 5 mm from the CTV. In addition, a 5 mm bolus was used over the chest wall. The IMN were contoured according to the Radiation Therapy Oncology Group breast cancer consensus: from the first to third intercostal spaces (ICS1–3) through the topography of the internal thoracic vessels (available online at: http://www.rtog.org/LinkClick.aspx?fileticket=vzJFPaBipE%3d&tabid=236). PTV IMN were designed to include an expansion of 5 mm around the IMN.

Treatment plan and dosimetric evaluation

The prescription dose was 50 Gy in 25 fractions (2 Gy per fraction) to the PTV, 5 days/week.

Three-dimensional conformal radiotherapy

The chest wall was treated with two opposite tangential fields (two oblique beams with an interval of 180°) using 6 MV photon beams and an ipsilateral SCF with a single anterior field. The criterion of the 3D-CRT plan was to ensure that at least 90% of the PTV received the prescription dose (Figure 1A).

Figure 1

3D-CRT, F-IMRT, and I-IMRT treatment plans.

Notes: (A) 3D-CRT, (B) F-IMRT, (C) I-IMRT.

Abbreviations: 3D-CRT, three-dimensional conformal radiotherapy; F-IMRT, field in-field forward intensity-modulated radiotherapy; I-IMRT, inverse intensity-modulated radiotherapy.

Forward intensity-modulated radiotherapy

The chest wall treatment plan involved using the tangential field technique with static multileaf collimator segments, with two parallel-opposed tangential fields using 6 MV photon beams. Two to five segmented fields were manipulated to maintain dose delivery to organs at risk (OARs), such as the IPSL, and heart within normally accepted tolerances and to reduce the volumes of hot spots in the treatment field. Four to five fields were designed toward the SCF to guarantee dose uniformity. The criterion of the F-IMRT plan was to ensure that at least 95% of the PTV received the prescription dose (Figure 1B).

Inverse intensity-modulated radiotherapy

The common isocenter was located in the center of the PTV. The tangential field technique was set to the entire PTV, and additional 0° and 40° multi-leaf collimator (MLC) segments were constructed toward the SCF. Additional subfields were set to reduce hot regions generated by the primary tangential fields and improve PTV dose uniformity to achieve dose homogeneity (Figure 1C).

Based on the patient’s dose-volume histogram (DVH), the mean doses (Dmean) and the volumes receiving equal to or more than 20, 30, 40, and 50 Gy (V20, V30, V40, and V50, respectively) to the IMN were analyzed.

Statistical analysis

Statistical analysis was performed with the SPSS statistical analysis software package. Based on the normality of the distributions, the Kruskal–Wallis H test was used for each dosimetric parameter in the three treatment plans. Wilcoxon signed-rank tests were used for comparisons of the dosimetric parameters of the first and third ICS. Spearman rank correlation test was used to examine the relationship between the dosimetric parameters of the IMN and the dosimetric parameters of the OARs. All tests were two sided. Data were regarded as statistically significant when P<0.05.

Results

Patients and treatment

One hundred and thirty-eight patients with breast cancer who received adjuvant postmastectomy RT (PMRT) at our institution were enrolled. Table 1 outlines the patient characteristics. One hundred and thirty-four patients were diagnosed with invasive ductal carcinoma, three patients with invasive lobular carcinoma, and one patient was diagnosed with invasive papillary carcinoma. A total of 48, 49, and 41 of the patients underwent 3D-CRT, F-IMRT, and I-IMRT followed by MRM, respectively.

Table 1

Patient and treatment characteristics

Characteristic	n	%
Age (years)	25–74
Median	47
Histology
Invasive ductal carcinoma	134	97.10
Invasive lobular carcinoma	3	2.17
Invasive papillary carcinoma	1	0.72
Tumor location
Left sided	73	52.90
Right sided	65	47.10
T stage
T0	2	1.45
T1	39	28.26
T2	79	57.25
T3	11	7.97
T4	6	4.35
Tx	1	0.72
N stage
N0	6	4.35
N1	46	33.33
N2	52	37.68
N3	33	23.91
Nx	1	0.72

Incidental IMN dose coverage

The Dmean to the IMN was 32.85 Gy for all patients. According to the treatment plan technique type, the Dmean values to the IMN in the patients undergoing 3D-CRT, F-IMTR, and I-IMRT were 33.80, 29.65, and 32.95 Gy, respectively (H=2.412, P=0.299). The detailed values of each parameter for the total IMN and the first ICS (ICS1), second ICS (ICS2), and third ICS (ICS3) are shown in Table 2. The Dmean to each ICS of the IMN in this study showed no differences among the 3D-CRT, F-IMRT, and I-IMRT groups. Representative axial images of all three plans, including target volume and field distributions, are shown in Figure 1.

Table 2

The dosimetric parameters for the IMN and each intercostal space of the IMN in the 3D-CRT, F-IMRT, and I-IMRT plans

	Mean (Gy)	V20 (%)	V30 (%)	V40 (%)	V50 (%)
3D-CRT
IMN	33.80	70.43	61.23	47.37	17.54
ICS1	27.78	58.29	45.08	30.20	6.00
ICS2	36.42	75.86	65.59	54.27	24.87
ICS3	38.43	86.57	66.87	59.43	26.39
F-IMRT
IMN	29.65	60.71	49.82	38.50	16.07
ICS1	24.96	50.13	36.96	26.69	5.85
ICS2	34.35	71.27	81.80	48.53	21.40
ICS3	34.57	76.32	64.26	53.82	18.29
I-IMRT
IMN	32.95	72.33	53.16	42.28	22.10
ICS1	26.49	59.43	33.73	21.53	7.94
ICS2	34.65	77.44	60.14	44.46	24.88
ICS3	39.21	93.29	69.00	59.09	31.03

Abbreviations: 3D-CRT, three-dimensional conformal radiotherapy; Dmean, the mean doses; F-IMRT, field in-field forward intensity-modulated radiotherapy; ICS, intercostal interspace; I-IMRT, inverse intensity-modulated radiotherapy; IMN, internal mammary nodes; Vx, the volumes receiving equal to or more than x Gy.

Thirty, 24, and 26 patients who underwent 3D-CRT, F-IMRT, and I-IMRT, respectively achieved 30 Gy for the incidental dose distribution to the IMN. Also, the incidental doses to the IMN of 10.42%, 2.04%, and 9.76% of the patients who underwent 3D-CRT, F-IMRT, and I-IMRT, respectively, reached 45 Gy. The IMN dosimetric parameters for each level of the 3D-CRT, F-IMRT, and I-IMRT plans are shown in Table 3, and the dosimetric parameters for each level of both the IMN and ICS1–3 were equal.

Table 3

Comparison of the IMN dosimetric parameters for each level in the 3D-CRT, F-IMRT, and I-IMRT plans

	3D-CRT	F-IMRT	I-IMRT	H	P-value
IMN (Gy)
≥45	10.42% (5)	2.04% (1)	9.76% (4)	0.020	0.990
~40	18.75% (9)	18.37% (9)	9.76% (4)
~35	14.58% (7)	8.16% (4)	19.51% (8)
~30	18.75% (9)	20.41% (10)	24.39% (10)
~25	16.67% (8)	22.45% (11)	14.63% (6)
ICS1 (Gy)
≥45	2.08% (1)	4.08% (2)	2.44% (1)	0.240	0.887
~40	6.25% (3)	2.04% (1)	7.32% (3)
~35	12.50% (6)	14.29% (7)	9.76% (4)
~30	20.83% (10)	20.41% (10)	19.51% (8)
~25	14.58% (7)	8.16% (4)	19.51% (8)
ICS2 (Gy)
≥45	25.00% (12)	14.29% (7)	21.95% (9)	1.269	0.530
~40	14.58% (7)	16.33% (8)	12.20% (5)
~35	12.50% (6)	16.33% (8)	14.63% (6)
~30	16.67% (8)	14.29% (7)	19.51% (8)
~25	14.58% (7)	8.16% (4)	14.63% (6)
ICS3 (Gy)
≥45	29.17% (14)	16.33% (8)	26.83% (11)	0.425	0.809
~40	18.75% (9)	20.41% (10)	19.51% (8)
~35	12.50% (6)	12.24% (6)	19.51% (8)
~30	14.58% (7)	20.41% (10)	7.32% (3)
~25	10.42% (5)	12.24% (6)	19.51% (8)

Abbreviations: 3D-CRT, three-dimensional conformal radiotherapy; F-IMRT, field in-field forward intensity-modulated radiotherapy; ICS, intercostal interspace; I-IMRT, inverse intensity-modulated radiotherapy; IMN, internal mammary nodes.

Regardless of the technique, the parameters, including Dmean, V20, V30, V40, and V50, indicated that the dose delivered to the IMN was lower in ICS1 than in ICS2 and ICS3. For 3D-CRT, F-IMRT, and I-IMRT, the Dmean values to the IMN in ICS2 and ICS3 were higher than 8.16/10.65, 9.39/9.61, and 8.16/12.72 Gy, respectively, compared to that in ICS1. For 3D-CRT, no significant differences were evident between ICS2 and ICS3. However, for patients who underwent F-IMRT, the V20, V30, and V40 in ICS3 were higher than those in ICS2 (Z=−2.053,−2.032, −2.021; P=0.040, 0.042, 0.043), and no differences were evident for Dmean and V50. For I-IMRT, ICS3 exceeded ICS2 in terms of Dmean, V20, V30, and V40 (Z=−2.287,−2.749, −4.840,−2.876; P=0.022, 0.006, 0.004, 0.004), and no difference was evident in V50 (Z=−1.626, P=0.104).

Analyses of OARs: the IPSL and heart

The OAR doses and volume results of the three groups are presented in Table 4. The IPSL volume for all breast cancer patients (P=0.290) and the heart volume for the left-sided breast cancer patients (P=0.985) were well balanced. Compared to the 3D-CRT plan, the F-IMRT and I-IMRT plans had decreased dose distributions to the IPSL (Dmean and V20). However, the dose delivered to the heart showed no differences in these plans with tangential techniques (Table 4). The correlation between the dose delivered to the IMN and OARs showed mixed features for 3D-CRT and IMRT (Table 5). A moderately positive correlation was found between the Dmean of the IMN and the Dmean of the heart for patients who underwent 3D-CRT (r=0.338, P=0.01). In addition, no dosimetric correction was found between the IMN and IPSL, whereas for the F-IMRT and I-IMRT groups, positive correlations were found only between the Dmean of IMN and the Dmean and V20 of the IPSL.

Table 4

Comparison of the lung and heart volume dosimetric parameters of the 3D-CRT, F-IMRT, and I-IMRT plans

	n	All patients			Left-sided cancer patients
		VL (cm3)	DmeanL (Gy)	V20L (Gy)	n	VH (cm3)	DmeanH (Gy)	V30H (Gy)
3D-CRT	48	1,208.77	16.17	30.13	20	527.47	8.14	10.76
F-IMRT	49	1,184.26	14.63	27.58	27	552.42	6.59	8.71
I-IMRT	41	1,189.15	15.34	26.99	26	501.81	6.97	8.73
H		2.475	7.756	6.143		0.031	5.108	2.023
P		0.290	0.021	0.046		0.985	0.078	0.364

Abbreviations: 3D-CRT, three-dimensional conformal radiotherapy; Dmean, the mean doses; F-IMRT, field in-field forward intensity-modulated radiotherapy; H, heart; I-IMRT, inverse intensity-modulated radiotherapy; L, lung; V, volume.

Table 5

The correlation between the dose delivered to the IMN and the OARs

		IMN					ICS1					ICS2					ICS3
		Dmean	V20	V30	V40	V50	Dmean	V20	V30	V40	V50	Dmean	V20	V30	V40	V50	Dmean	V20	V30	V40	V50
3D-CRT
L Dmean	r	0.271	0.268	0.252	0.247	0.186	0.339	0.317	0.338	0.360	0.373	0.184	0.180	0.169	0.217	0.168	0.148	0.179	0.120	0.149	0.109
	P-value	0.062	0.065	0.084	0.091	0.207	0.018	0.028	0.019	0.012	0.009	0.212	0.220	0.251	0.138	0.253	0.317	0.223	0.417	0.313	0.460
L V20	r	0.250	0.263	0.243	0.228	0.148	0.364	0.345	0.366	0.379	0.361	0.138	0.140	0.121	0.176	0.115	0.134	0.166	0.116	0.138	0.083
	P-value	0.086	0.071	0.096	0.119	0.317	0.011	0.016	0.010	0.008	0.012	0.348	0.344	0.411	0.232	0.435	0.363	0.259	0.430	0.350	0.574
H Dmean	r	0.338	0.339	0.332	0.320	0.333	0.373	0.372	0.361	0.365	0.378	0.217	0.195	0.219	0.188	0.227	0.409	0.376	0.385	0.438	0.463
	P-value	0.019	0.018	0.021	0.027	0.021	0.009	0.009	0.012	0.011	0.008	0.138	0.183	0.134	0.201	0.121	0.004	0.008	0.007	0.002	0.001
H V30	r	0.163	0.173	0.164	0.153	0.231	0.195	0.191	0.189	0.197	0.209	0.064	0.038	0.073	0.059	0.107	0.291	0.250	0.261	0.322	0.386
	P-value	0.269	0.241	0.267	0.298	0.114	0.185	0.193	0.197	0.180	0.154	0.664	0.796	0.624	0.693	0.468	0.045	0.087	0.073	0.026	0.007
F-IMRT
L Dmean	r	0.366	0.384	0.352	0.318	0.362	0.408	0.396	0.386	0.386	0.381	0.295	0.275	0.277	0.272	0.219	0.262	0.266	0.270	0.268	0.224
	P-value	0.010	0.007	0.013	0.026	0.011	0.004	0.005	0.006	0.006	0.007	0.040	0.056	0.054	0.059	0.131	0.069	0.065	0.060	0.063	0.122
L V20	r	0.318	0.335	0.303	0.262	0.278	0.376	0.38	0.368	0.365	0.340	0.237	0.220	0.222	0.214	0.148	0.213	0.224	0.226	0.217	0.153
	P-value	0.026	0.019	0.034	0.069	0.053	0.008	0.007	0.009	0.010	0.017	0.101	0.129	0.126	0.141	0.310	0.141	0.123	0.118	0.134	0.293
H Dmean	r	0.102	0.128	0.122	0.111	−0.041	0.173	0.144	0.146	0.126	0.105	0.049	0.047	0.048	0.056	0.067	0.174	0.203	0.192	0.208	0.104
	P-value	0.486	0.380	0.404	0.448	0.778	0.235	0.325	0.318	0.389	0.474	0.740	0.747	0.745	0.703	0.648	0.231	0.162	0.186	0.151	0.477
H V30	r	−0.057	−0.046	−0.037	−0.047	−0.206	0.014	−0.035	−0.023	−0.038	−0.044	−0.105	−0.108	−0.102	−0.092	−0.077	0.036	0.060	0.056	0.070	−0.03
	P-value	0.695	0.752	0.799	0.750	0.156	0.925	0.81	0.878	0.795	0.764	0.472	0.459	0.484	0.531	0.597	0.806	0.683	0.703	0.631	0.839
I-IMRT
L Dmean	r	0.427	0.356	0.408	0.410	0.361	0.399	0.273	0.356	0.352	0.259	0.383	0.410	0.384	0.336	0.323	0.333	0.388	0.380	0.310	0.268
	P-value	0.005	0.022	0.008	0.008	0.021	0.010	0.084	0.022	0.024	0.102	0.013	0.008	0.013	0.032	0.039	0.033	0.012	0.014	0.049	0.090
L V20	r	0.411	0.359	0.372	0.379	0.339	0.396	0.309	0.358	0.356	0.253	0.360	0.390	0.349	0.320	0.317	0.260	0.282	0.274	0.202	0.230
	P-value	0.008	0.021	0.017	0.015	0.030	0.010	0.049	0.021	0.022	0.111	0.021	0.012	0.026	0.041	0.043	0.100	0.074	0.083	0.205	0.148
H Dmean	r	0.277	0.286	0.236	0.218	0.158	0.290	0.212	0.223	0.176	0.306	0.151	0.214	0.128	0.060	0.046	0.300	0.346	0.308	0.290	0.146
	P-value	0.080	0.070	0.138	0.170	0.324	0.066	0.184	0.162	0.271	0.052	0.346	0.178	0.425	0.708	0.777	0.057	0.027	0.050	0.066	0.362
H V30	r	0.220	0.222	0.191	0.189	0.148	0.216	0.148	0.202	0.189	0.307	0.117	0.171	0.107	0.043	0.014	0.274	0.297	0.250	0.256	0.166
	P-value	0.167	0.163	0.230	0.236	0.356	0.176	0.355	0.205	0.237	0.051	0.468	0.286	0.504	0.792	0.928	0.082	0.059	0.115	0.106	0.301

Abbreviations: 3D-CRT, three-dimensional conformal radiotherapy; Dmean, the mean doses; F-IMRT, forward intensity-modulated radiotherapy; H, heart; ICS, intercostal interspace; IMN, internal mammary nodes; I-IMRT, inverse intensity-modulated radiotherapy; L, lung; Vx, the volumes receiving equal to or more than x Gy.

Discussion

The incidental irradiated dosimetric reviews of the IMN from the published articles described above are summarized in Table 6. Our study showed a relatively similar Dmean in the IMN region. Notably, these trials only used the 3D-CRT technique on the targeted volume. To the best of our knowledge, our study on IMN incidental irradiation coverage is the first to address this topic using three different techniques and to examine these dose distribution patterns and OAR dose correlations. Compared to 3D-CRT, the improved conformity of IMRT indicates that less irradiation was delivered outside of the PTV. Conventional parallel-opposed tangential fields were still mainly used in F-IMRT and I-IMRT, and the IMN were in the rear of the inner tangential semi-opposed beams. Therefore, the IMN were incidentally irradiated with an equal dose of radiation to that of 3D-CRT. This finding indicates that in every analysis we performed concerning these three RT techniques, no group ultimately received an adequate or effective prophylactic treatment. Adequate coverage of the IMN, defined as ≥45 Gy, was achieved in 10.42%, 2.04%, and 9.76% of the patients with 3D-3CRT, F-IMRT, and I-IMRT techniques, respectively. Thus, tailored RT for individual patients might be needed.

Table 6

Summary of IMN doses in the 3D-CRT plans that were previously published

	MRM	MRM + R	BCS
Kanyilmaz et al24	34.6 Gy	–	26.7 Gy
Sapienza et al25	30.34 Gy	30.26 Gy	18.67 Gy
Arora et al26	24.98 Gy (ICS1–5); 30.49 Gy (ICS1–3)	–	–
Chung et al27	61.3%±21.1% of the prescription dose	–	57.3%±17.1% of the prescription dose
Leite et al28	20.64 Gy (57.5% BCS, 27.5% MRM, 15.0% MRM + R)

Abbreviations: 3D-CRT, three-dimensional conformal radiotherapy; BCS, breast conservation surgery; ICS, intercostal interspace; IMN, internal mammary nodes; MRM, modified radical mastectomy; MRM + R, modified radical mastectomy with immediate reconstruction.

Previous reports have shown that relatively low doses (10–30 Gy) can sterilize subclinical metastases and microscopic tumors of ovarian, bladder, and breast carcinomas and of head and neck neoplasms.28,29 Recently, Lee et al found that for breast cancer, the estimated 50% tumor control dose (TCD50) values were 19.3 Gy for MCF7 (luminal) cells and 44.9 Gy for SUM159 (basal) cells.30 In addition, of the enrolled patients in our study, 62.5%, 48.98%, and 63.41% achieved 30 Gy for the incidental dose distribution to the IMN and 10.42%, 2.04%, and 9.76% of the patients who underwent 3D-CRT, F-IMRT, and I-IMRT, respectively, reached 45 Gy. Therefore, for invasive breast carcinoma, the incidental dose to the IMN does not reach standard clinically therapeutic levels in MRM breast cancer patients treated with any of these three RT techniques, but a worthwhile benefit can be achieved by the incidental doses delivered when only chest wall ± SCF are irradiated without the IMN region. This finding is also confirmed by the overall low recurrence rates in the IMN after PMRT, even when these nodes are not excised or irradiated.22,23 The European Organization for Research and Treatment of Cancer (EORTC) Radiation Oncology and Breast Cancer groups found that for patients with stage I, II, and III cancers with centrally or medially located primary tumors, in the whole breast irradiation (WBI) or chest wall irradiation alone group, the internal mammary recurrence rate was 0.8%, and in the nodal irradiation group, the rate was 0.2%.23 Kanyilmaz et al showed that the incidental Dmean to the IMN in patients treated with 3D-CRT after MRM was 32.8 Gy, and that advanced T and N stages were the prognostic factors that affected OS and progression-free survival, which were poorly affected by unplanned irradiation doses to the IMN.24 No IMN relapses occurred during the median 38-month (range 3–80) follow-up time.

In our hospital, internal mammary SLN (IM-SLN) biopsy was performed with the modified injection technique, and the IM-SLNs were detected by preoperative lymphoscintigraphy and/or intraoperative gamma probe detection. The results showed that the IM-SLNs were concentrated in the second and third ICS (80.8%). ICS3 had the highest incidence of positive IMN, followed by the second and first spaces (50.2%, 30.6%, and 1.6% for ICS3, ICS2, and ICS1, respectively).31 Krishan et al analyzed the anatomical distribution of IMN metastases and found that 78% of nodal metastases were in the first three ICSs, whereas 14% and 8% were located in ICS3 and ICS1, respectively.32 The regularity of the distributions of the IMN metastases was consistent with that of IM-SLN metastases. Our results showed that regardless of the RT technique, the Dmean values to the IMN in ICS2 and ICS3 were higher than 8.16–12.72 Gy compared to that of ICS1. In addition, the dose may be conducive for detecting the ICS2 and ICS3 recurrence rate. The anatomical position and topography of the internal thoracic vessels in ICS1 are deeper than those in ICS2 and ICS3, which may lead to the IMN dose differences of the different ICSs. Sapienza et al evaluated the relationship between the unintentional coverage of the IMN and the type of surgery employed, and the authors found that the Dmean values to the IMN after MRM and MRM with immediate reconstruction (MRM + R) were greater than those for BCS (30.34 Gy for MRM, 30.26 Gy for MRM + R, and 18.67 Gy for BCS).25 MRM changed the chest wall thickness, which resulted in the variation in IMN depth, possibly leading to the IMN dose differences of each operative approach.

Darby et al reconstructed the RT regimens of 2,168 women on computed tomography scans with typical anatomy and found a dose-dependent increase in the risk of late ischemic heart disease associated with RT for cancer of the left breast.33 We also found that for patients who underwent 3D-CRT, the incidental dose to the IMN was positively correlated with the Dmean of the heart. In addition, this moderate positive correlation also existed in ICS3. However, in our stratified analyses, little of the heterogeneity was explained by the IPSL volume for all breast cancer patients or the heart volume for left-sided early breast cancer patients. With equal dose distribution patterns for all three techniques, the Dmean of the heart was further decreased by ~2 Gy in the IMRT cohort (F-IMRT and I-IMRT), and no correlation was evident between the incidental dose to the IMN and the dose to the heart. Contemporary IMRT likely reduced the risk to the heart, and RT-induced cardiotoxicity may have been mitigated by the medical progress made in the treatment of ischemic heart disease over the past decade. Therefore, the incubation time of late cardiac complications may be prolonged,34 so the long-term hazards in the general population will still need to be monitored with additional follow-up and summaries in most cases.

EORTC trial 22922/10925 found that lung toxicity was significantly increased with irradiation of the IMN and SCF treatment (fibrosis, 0.9% vs 2.8%; dyspnea, 0.1% vs 0.7%; pneumonitis, 0.1% vs 0.7%; any lung toxicity, 4.3% vs 1.3%).17 In our study, although the IMN were not included in the treatment volume, a significant association was evident between the incidental dose to the IMN and the Dmean and V20 to the IPSL for IMRT. Therefore, the next research direction will involve identifying the influencing factors of the incidental coverage to the IMN and screening out potential breast cancer patients whose incidental IMN doses can achieve clinically therapeutic levels that do not require IMN RT.

Conclusion

F-IMRT and I-IMRT can deliver lower and clinically less relevant doses than 3D-CRT to OARs. The incidental dose to the IMN of patients treated with 3D-CRT, F-IMRT, and I-IMRT were equal when the IMN were routinely uninvolved in the target volume. However, a higher incidental dose to the IMN was associated with a higher Dmean to the heart for 3D-CRT, and a higher incidental dose to the IMN was associated with a higher dose to the IPSL for patients who underwent F-IMRT and I-IMRT. Although none of these three RT techniques ultimately achieved an adequate and effective prophylactic treatment for the IMN, a substantial dose was still delivered to the IMN for partial patients. A more careful data analysis or long-term clinical trial is warranted for detailed explorations of the subgroups that do not require IMN irradiation, and additional follow-up is needed to determine whether the different incidental dose to the IMN would result in a different recurrence rate in the IMN.