Yufan Wang1,2, Beibei Liu2, Yong Yang3, Yamin Wang2, Zhong Zhao4, Zhigang Miao2,5, Jiangtao Zhu1. 1. Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou City, Jiangsu, China. 2. Institute of Neuroscience, Soochow University, Suzhou City, China. 3. Department of psychiatry, The Affiliated Guangji Hospital of Soochow University, Suzhou, China. 4. Department of Neurology, The Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou, China. 5. Jiangsu Key Laboratory of Neuropsychiatric Diseases, Soochow University, Suzhou, Jiangsu 215123, China.
Abstract
Aim: We aim to study the antidepressant mechanism of metformin. Materials & methods: Tail suspension test and forced swimming test were used to detect the depression-like behavior; the expressions of target protein were examined by western blot; the levels of target genes were tested by quantitative PCR; the content of α-ketoglutarate and 5hmC were detected by ELISA kit. Results: We showed that metformin can improve the depression-like behavior in spatial restraint stress model; then we found that metformin through AMPK/Tet2 pathway increasing the expression of BDNF to antidepression. Conclusion: Our study provided evidences that metformin plays a role of antidepressant effects through the AMPK/Tet2/BDNF pathway.
Aim: We aim to study the antidepressant mechanism of metformin. Materials & methods: Tail suspension test and forced swimming test were used to detect the depression-like behavior; the expressions of target protein were examined by western blot; the levels of target genes were tested by quantitative PCR; the content of α-ketoglutarate and 5hmC were detected by ELISA kit. Results: We showed that metformin can improve the depression-like behavior in spatial restraint stress model; then we found that metformin through AMPK/Tet2 pathway increasing the expression of BDNF to antidepression. Conclusion: Our study provided evidences that metformin plays a role of antidepressant effects through the AMPK/Tet2/BDNF pathway.
Authors: Mahmoud S Abdallah; Esraa M Mosalam; Abdel-Aziz A Zidan; Khaled S Elattar; Shimaa A Zaki; Ahmed N Ramadan; Abla M Ebeid Journal: Neurotherapeutics Date: 2020-10 Impact factor: 6.088