| Literature DB >> 30742997 |
Monica Manglani1, Rejane Rua1, Amy Hendricksen2, Daniel Braunschweig2, Qian Gao2, Woei Tan2, Brett Houser2, Dorian B McGavern1, Kenneth Oh2.
Abstract
This protocol describes how to prepare mouse brain tissue for quantification of multiple inflammatory mediators using a multiplex bead-based immunoassay. It is important to have methods that allow quantification of multiple analytes from small amounts of tissue. Bio-Plex is a Luminex xMAP-based multiplex bead-based immunoassay technology that permits simultaneous analysis of up to 100 analytes from a single tissue sample. This assay has been used extensively to investigate analytes in plasma and serum samples as well as cultured and primary cells. Here, we describe a method for simultaneous analysis of 33 different inflammatory cytokines and chemokines from mouse brain tissue using the Bio-Plex Pro Mouse Chemokine Panel 33-Plex.Entities:
Keywords: Bio-Plex; Cerebral Malaria; Chemokine; Cytokine; Mouse brain; Plasmodium berghei
Mesh：
Substances：
Year: 2019 PMID： 30742997 PMCID： PMC6802274 DOI： 10.1016/j.ymeth.2019.02.007
Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608