Literature DB >> 307289

A double-antibody radioimmunoassay for serum progesterone using progesterone-3-(O-carboxymethyl) oximino-[125I]-iodo-histamine as radioligand.

J Z Scott, F Z Stanczyk, U Goebelsmann, D R Mishell.   

Abstract

A reliable, convenient and economical radioimmunoassay (RIA) for serum progesterone has been established and tested. This procedure employs diethyl ether extraction followed by RIA utilizing rabbit anti-11 alpha-hydroxyprogesterone 11-hemisuccinyl-bovine serum albumin (progesterone-11 alpha-BSA) serum, progresterone-3-(O-carboxymethyl) oximino-[125I]-iodohistamine (progesterone-3-[125I]) as radioligand and goat anti-rabbit gamma globulin as second antibody. In conjunction with antiprogesterone-11 alpha-BSA serum, the overall assay specificity of the progesterone-3-[125I] RIA is similar to that of the [3H]-progesterone method using dextran-coated charcoal. The results of serum progesterone measurements during the menstrual cycle obtained by the progesterone-3-[125I] RIA appear comparable to those of [3H]-progesterone assays which employ similar anti-progesterone-11 alpha-BSA sera. The progesterone-3-[125I] double-antibody RIA, however, is more convenient and less expensive than the [3H]-progesterone RIA method.

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Year:  1978        PMID: 307289     DOI: 10.1016/0039-128x(78)90052-1

Source DB:  PubMed          Journal:  Steroids        ISSN: 0039-128X            Impact factor:   2.668


  14 in total

1.  Adolescent diet and subsequent serum hormones, breast density, and bone mineral density in young women: results of the Dietary Intervention Study in Children follow-up study.

Authors:  Joanne F Dorgan; Lea Liu; Catherine Klifa; Nola Hylton; John A Shepherd; Frank Z Stanczyk; Linda G Snetselaar; Linda Van Horn; Victor J Stevens; Alan Robson; Peter O Kwiterovich; Norman L Lasser; John H Himes; Kelley Pettee Gabriel; Andrea Kriska; Elizabeth H Ruder; Carolyn Y Fang; Bruce A Barton
Journal:  Cancer Epidemiol Biomarkers Prev       Date:  2010-05-25       Impact factor: 4.254

2.  Serum hormone levels in pre-menopausal Chinese women in Shanghai and white women in Los Angeles: results from two breast cancer case-control studies.

Authors:  L Bernstein; J M Yuan; R K Ross; M C Pike; R Hanisch; R Lobo; F Stanczyk; Y T Gao; B E Henderson
Journal:  Cancer Causes Control       Date:  1990-07       Impact factor: 2.506

3.  Associations of pregnancy characteristics with maternal and cord steroid hormones, angiogenic factors, and insulin-like growth factor axis.

Authors:  Jessica M Faupel-Badger; Yuping Wang; S Ananth Karumanchi; Frank Stanczyk; Michael Pollak; Thomas McElrath; Robert N Hoover; Rebecca Troisi
Journal:  Cancer Causes Control       Date:  2011-09-24       Impact factor: 2.506

4.  Progestogen levels, progesterone receptor gene polymorphisms, and mammographic density changes: results from the Postmenopausal Estrogen/Progestin Interventions Mammographic Density Study.

Authors:  Eunjung Lee; Sue A Ingles; David Van Den Berg; Wei Wang; Chris Lavallee; Mei-Hua Huang; Carolyn J Crandall; Frank Z Stanczyk; Gail A Greendale; Giske Ursin
Journal:  Menopause       Date:  2012-03       Impact factor: 2.953

5.  Circulating sex hormones and mammographic breast density among postmenopausal women.

Authors:  Brian L Sprague; Amy Trentham-Dietz; Ronald E Gangnon; Diana S M Buist; Elizabeth S Burnside; Erin J Aiello Bowles; Frank Z Stanczyk; Gale S Sisney
Journal:  Horm Cancer       Date:  2011-02       Impact factor: 3.869

6.  Endogenous sex hormones and breast density in young women.

Authors:  Seungyoun Jung; Frank Z Stanczyk; Brian L Egleston; Linda G Snetselaar; Victor J Stevens; John A Shepherd; Linda Van Horn; Erin S LeBlanc; Kenneth Paris; Catherine Klifa; Joanne F Dorgan
Journal:  Cancer Epidemiol Biomarkers Prev       Date:  2014-11-04       Impact factor: 4.254

7.  Sex hormone levels and risk of breast cancer with estrogen plus progestin.

Authors:  Ghada N Farhat; Neeta Parimi; Rowan T Chlebowski; Joann E Manson; Garnet Anderson; Alison J Huang; Eric Vittinghoff; Jennifer S Lee; Andrea Z Lacroix; Jane A Cauley; Rebecca Jackson; Deborah Grady; Dorothy S Lane; Lawrence Phillips; Michael S Simon; Steven R Cummings
Journal:  J Natl Cancer Inst       Date:  2013-09-16       Impact factor: 13.506

8.  Serum factors and clinical characteristics associated with serum E-screen activity.

Authors:  Jue Wang; Amy Trentham-Dietz; Jocelyn D C Hemming; Curtis J Hedman; Brian L Sprague
Journal:  Cancer Epidemiol Biomarkers Prev       Date:  2013-04-15       Impact factor: 4.254

9.  A Prospective Evaluation of Endogenous Sex Hormone Levels and Colorectal Cancer Risk in Postmenopausal Women.

Authors:  Neil Murphy; Howard D Strickler; Frank Z Stanczyk; Xiaonan Xue; Sylvia Wassertheil-Smoller; Thomas E Rohan; Gloria Y F Ho; Garnet L Anderson; John D Potter; Marc J Gunter
Journal:  J Natl Cancer Inst       Date:  2015-08-01       Impact factor: 13.506

10.  Circulating maternal and umbilical cord steroid hormone and insulin-like growth factor concentrations in twin and singleton pregnancies.

Authors:  L C Houghton; M Lauria; P Maas; F Z Stanczyk; R N Hoover; R Troisi
Journal:  J Dev Orig Health Dis       Date:  2018-10-08       Impact factor: 2.401

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