| Literature DB >> 30728416 |
Jan P Gerlach1,2, Jessie A G van Buggenum1, Sabine E J Tanis1, Mark Hogeweg1, Branco M H Heuts1, Mauro J Muraro3, Lisa Elze1, Francesca Rivello4, Agata Rakszewska4, Alexander van Oudenaarden3, Wilhelm T S Huck4, Hendrik G Stunnenberg2, Klaas W Mulder5.
Abstract
Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogeneous cellular responses to environmental signals at the mRNA and phospho-proteome level.Entities:
Mesh：
Substances：
Year: 2019 PMID： 30728416 PMCID： PMC6365588 DOI： 10.1038/s41598-018-37977-7
Source DB: PubMed Journal: Sci Rep ISSN： 2045-2322 Impact factor: 4.379