Literature DB >> 30725464

Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells.

Jing Zhang1, Qing Zhang2,3.   

Abstract

A large amount of energy used for nutrient processing and cellular functions is essential for tumorigenesis. Total intracellular adenosine triphosphate (ATP) is mainly generated by glycolysis and mitochondrial oxidative phosphorylation. Here, we provide a protocol for measurements of energy metabolism in cancer cells by using Seahorse XF24 Extracellular Flux analyzer. Specifically, this machine measures glycolysis by analyzing the extracellular acidification rate (ECAR) and measures mitochondrial oxidative phosphorylation on the basis of the oxygen consumption rate (OCR), through real-time and live cell analysis. This protocol is provided for researchers who are unfamiliar with the method and to aid them in carrying out the technique successfully.

Entities:  

Keywords:  ECAR; Energy metabolism; Glycolysis; OCR; Oxidative phosphorylation; Seahorse XF24 Extracellular Flux analyzer

Mesh：

Year:  2019        PMID： 30725464     DOI： 10.1007/978-1-4939-9027-6_18

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  20 in total

1.  SLC-30A9 is required for Zn2+ homeostasis, Zn2+ mobilization, and mitochondrial health.

Authors:  Huichao Deng; Xinhua Qiao; Ting Xie; Wenfeng Fu; Hang Li; Yanmei Zhao; Miaomiao Guo; Yaqian Feng; Ligong Chen; Yan Zhao; Long Miao; Chang Chen; Kang Shen; Xiangming Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2021-08-31       Impact factor: 11.205

2.  An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.

Authors:  Jin Zhou; Jeremy M Simon; Chengheng Liao; Cheng Zhang; Lianxin Hu; Giada Zurlo; Xijuan Liu; Cheng Fan; Austin Hepperla; Liwei Jia; Vanina Toffessi Tcheuyap; Hua Zhong; Roy Elias; Jin Ye; W Mike Henne; Payal Kapur; Deepak Nijhawan; James Brugarolas; Qing Zhang
Journal:  Mol Cell       Date:  2022-06-27       Impact factor: 19.328

3.  Characterizing the metabolic profile of dexamethasone treated human trabecular meshwork cells.

Authors:  Kimberly Graybeal; Luis Sanchez; Chi Zhang; Linsey Stiles; Jie J Zheng
Journal:  Exp Eye Res       Date:  2021-12-09       Impact factor: 3.770

4.  Mitochondrial Function and Metabolism of Cultured Skeletal Cells.

Authors:  Li Tian; Clifford J Rosen; Anyonya R Guntur
Journal:  Methods Mol Biol       Date:  2021

5.  Targeting redox heterogeneity to counteract drug tolerance in replicating Mycobacterium tuberculosis.

Authors:  Richa Mishra; Sakshi Kohli; Nitish Malhotra; Parijat Bandyopadhyay; Mansi Mehta; MohamedHusen Munshi; Vasista Adiga; Vijay Kamal Ahuja; Radha K Shandil; Raju S Rajmani; Aswin Sai Narain Seshasayee; Amit Singh
Journal:  Sci Transl Med       Date:  2019-11-13       Impact factor: 17.956

6.  UBQLN1 mediates sorafenib resistance through regulating mitochondrial biogenesis and ROS homeostasis by targeting PGC1β in hepatocellular carcinoma.

Authors:  Junjie Xu; Lin Ji; Yeling Ruan; Zhe Wan; Zhongjie Lin; Shunjie Xia; Liye Tao; Junhao Zheng; Liuxin Cai; Yifan Wang; Xiao Liang; Xiujun Cai
Journal:  Signal Transduct Target Ther       Date:  2021-05-18

Review 7.  A guide to interrogating immunometabolism.

Authors:  Kelsey Voss; Hanna S Hong; Jackie E Bader; Ayaka Sugiura; Costas A Lyssiotis; Jeffrey C Rathmell
Journal:  Nat Rev Immunol       Date:  2021-04-15       Impact factor: 108.555

8.  Loss of Function of the Gene Encoding the Histone Methyltransferase KMT2D Leads to Deregulation of Mitochondrial Respiration.

Authors:  Consiglia Pacelli; Iolanda Adipietro; Natascia Malerba; Gabriella Maria Squeo; Claudia Piccoli; Angela Amoresano; Gabriella Pinto; Pietro Pucci; Ji-Eun Lee; Kai Ge; Nazzareno Capitanio; Giuseppe Merla
Journal:  Cells       Date:  2020-07-13       Impact factor: 6.600

9.  N-Alkylation of Anthracycline Antibiotics by Natural Sesquiterpene Lactones as a Way to Obtain Antitumor Agents with Reduced Side Effects.

Authors:  Margarita Neganova; Alexey Semakov; Yulia Aleksandrova; Ekaterina Yandulova; Sergey Pukhov; Lada Anikina; Sergey Klochkov
Journal:  Biomedicines       Date:  2021-05-13

10.  Glucocorticoid receptor triggers a reversible drug-tolerant dormancy state with acquired therapeutic vulnerabilities in lung cancer.

Authors:  Stefan Prekovic; Karianne Schuurman; Isabel Mayayo-Peralta; Anna G Manjón; Mark Buijs; Selçuk Yavuz; Max D Wellenstein; Alejandro Barrera; Kim Monkhorst; Anne Huber; Ben Morris; Cor Lieftink; Theofilos Chalkiadakis; Ferhat Alkan; Joana Silva; Balázs Győrffy; Liesbeth Hoekman; Bram van den Broek; Hans Teunissen; Donna O Debets; Tesa Severson; Jos Jonkers; Timothy Reddy; Karin E de Visser; William Faller; Roderick Beijersbergen; Maarten Altelaar; Elzo de Wit; Rene Medema; Wilbert Zwart
Journal:  Nat Commun       Date:  2021-07-16       Impact factor: 14.919
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