| Literature DB >> 30721790 |
María Del Pilar Chantada-Vázquez1, Antonio Castro López2, Susana B Bravo3, Sergio Vázquez-Estévez4, Benigno Acea-Nebril5, Cristina Núñez6.
Abstract
Adsorption of biomolecules onto nanoparticles surface in biological samples led to the formation of a bio-corona, it could modified the "identity" of nanoparticles, contributing to the determination of their toxicity and biocompatibility. Gel electrophoresis in combination with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to qualitatively analyze and identify the human serum proteins adsorbed on the surface of three different nanomaterials stabilized with citrate: 10.02 ± 0.91 nm gold nanoparticles (AuNPs), 9.73 ± 1.70 nm silver nanoparticles (AgNPs) and, 2.40 ± 0.30 nm platinum nanoparticles (PtNPs). An exhaustive analysis and classification of all identified proteins related with their function were also developed.Entities:
Keywords: Gold nanoparticles (AuNPs); Human serum; Platinum nanoparticles (PtNPs); Prognostic biomarker; Protein corona; Silver nanoparticles (AgNPs)
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Year: 2019 PMID： 30721790 DOI： 10.1016/j.colsurfb.2019.01.056
Source DB: PubMed Journal: Colloids Surf B Biointerfaces ISSN： 0927-7765 Impact factor: 5.268