| Literature DB >> 33687711 |
Maria Del Pilar Chantada-Vázquez1, María García Vence2, Antonio Serna3, Cristina Núñez4, Susana B Bravo5.
Abstract
Identification of molecular biomarkers for human diseases is one of the most important disciplines in translational science as it helps to elucidate their origin and early progression. Thus, it is a key factor in better diagnosis, prognosis, and treatment. Proteomics can help to solve the problem of sample complexity when the most common primary sample specimens were analyzed: organic fluids of easy access. The latest developments in high-throughput and label-free quantitative proteomics (SWATH-MS), together with more advanced liquid chromatography, have enabled the analysis of large sample sets with the sensitivity and depth needed to succeed in this task. In this chapter, we show different sample processing methods (major protein depletion, digestion, etc.) and a micro LC-SWATH-MS protocol to identify/quantify several proteins in different types of samples (serum/plasma, saliva, urine, tears).Entities:
Keywords: Biological fluids; Label-free quantification; Nanoproteomics; SWATH-MS; Saliva; Serum/plasma; Tears; Urine
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Year: 2021 PMID： 33687711 DOI： 10.1007/978-1-0716-1178-4_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745