Literature DB >> 3071540

Analysis of Salmonella lipopolysaccharides by sodium deoxycholate-polyacrylamide gel electrophoresis.

T Komuro1, C Galanos.   

Abstract

Lipopolysaccharides (LPSs) were analyzed by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium deoxycholate (DOC) or sodium dodecyl sulphate (SDS) and the results obtained compared. Two-dimensional PAGE of the S form of LPS revealed that, with SDS, bands which appeared to be single after the one-dimensional experiment were resolved into several bands after two-dimensional electrophoresis. In the presence of DOC, however, a diagonal of single bands was obtained in one-dimensional electrophoresis indicating optimum resolution. The high quality of resolution by DOC-PAGE was constant for amounts of LPS up to 20 micrograms. Finally, DOC-PAGE does not require boiling of the samples in DOC, which may be an advantage over SDS-PAGE.

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Year:  1988        PMID： 3071540     DOI： 10.1016/s0021-9673(01)83593-7

Source DB:  PubMed          Journal:  J Chromatogr


  12 in total

1.  Use of two-dimensional gas chromatography with electron-capture detection for the measurement of lipopolysaccharides in peritoneal fluid and plasma from rats with induced peritonitis.

Authors:  A Sonesson; L Larsson; R Andersson; N Adner; K G Tranberg
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

2.  Fluorescence resonance energy transfer analysis of lipopolysaccharide in detergent micelles.

Authors:  C A Wiström; G M Jones; P S Tobias; L A Sklar
Journal:  Biophys J       Date:  1996-02       Impact factor: 4.033

3.  A novel component different from endotoxin extracted from Prevotella intermedia ATCC 25611 activates lymphoid cells from C3H/HeJ mice and gingival fibroblasts from humans.

Authors:  K Iki; K Kawahara; S Sawamura; R Arakaki; T Sakuta; A Sugiyama; H Tamura; T Sueda; S Hamada; H Takada
Journal:  Infect Immun       Date:  1997-11       Impact factor: 3.441

4.  Structural elucidation of the lipopolysaccharide core region of the O-chain-deficient mutant strain A28 from Pseudomonas aeruginosa serotype 06 (International Antigenic Typing Scheme).

Authors:  H Masoud; I Sadovskaya; T de Kievit; E Altman; J C Richards; J S Lam
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

5.  Lipopolysaccharides of Bacteroides intermedius (Prevotella intermedia) and Bacteroides (Porphyromonas) gingivalis induce interleukin-8 gene expression in human gingival fibroblast cultures.

Authors:  M Tamura; M Tokuda; S Nagaoka; H Takada
Journal:  Infect Immun       Date:  1992-11       Impact factor: 3.441

6.  Production and characterization of monoclonal antibodies to Actinobacillus pleuropneumoniae serotype 5b.

Authors:  E Altman; M A Gidney; B A Harrison; M Gottschalk
Journal:  Can J Vet Res       Date:  1995-10       Impact factor: 1.310

7.  A novel fluorescent probe that senses the physical state of lipid bilayers.

Authors:  Hirotaka Sasaki; Stephen H White
Journal:  Biophys J       Date:  2009-06-03       Impact factor: 4.033

8.  Chemical characterization of two lipopolysaccharide species isolated from Rhizobium loti NZP2213.

Authors:  R Russa; T Urbanik-Sypniewska; K Lindström; H Mayer
Journal:  Arch Microbiol       Date:  1995-05       Impact factor: 2.552

9.  Lipopolysaccharide of Rhodospirillum salinarum 40: structural studies on the core and lipid A region.

Authors:  H Rau; U Seydel; M Freudenberg; J Weckesser; H Mayer
Journal:  Arch Microbiol       Date:  1995-10       Impact factor: 2.552

10.  Characterization of lipopolysaccharide heterogeneity in Salmonella enteritidis by an improved gel electrophoresis method.

Authors:  J Guard-Petter; B Lakshmi; R Carlson; K Ingram
Journal:  Appl Environ Microbiol       Date:  1995-08       Impact factor: 4.792
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