Literature DB >> 8548689

Production and characterization of monoclonal antibodies to Actinobacillus pleuropneumoniae serotype 5b.

E Altman1, M A Gidney, B A Harrison, M Gottschalk.   

Abstract

Monoclonal antibodies specific for capsular polysaccharide (CPS) and lipopolysaccharide (LPS) of A. pleuropneumoniae serotype 5b were generated by hybridoma cells and selected by indirect ELISA of culture supernatants with purified and structurally defined LPS and CPS preparations and their synthetic conjugates. It was shown in this study that at least one monoclonal antibody, 3B4, presented 100% specificity and recognized all A. pleuropneumoniae serotype 5 field strains tested in a dot-ELISA assay.

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Year:  1995        PMID： 8548689      PMCID： PMC1263782     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  26 in total

1.  An evaluation of agglutination and coagglutination techniques for serotyping of Haemophilus pleuropneumoniae isolates.

Authors:  K R Mittal; R Higgins; S Larivière
Journal:  Am J Vet Res       Date:  1987-02       Impact factor: 1.156

2.  Improved techniques for the preparation of bacterial lipopolysaccharides.

Authors:  K G Johnson; M B Perry
Journal:  Can J Microbiol       Date:  1976-01       Impact factor: 2.419

3.  A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.

Authors:  C M Tsai; C E Frasch
Journal:  Anal Biochem       Date:  1982-01-01       Impact factor: 3.365

4.  Serotyping and detection of Haemophilus pleuropneumoniae by indirect fluorescent antibody technique.

Authors:  S Rosendal; L Lombin; J DeMoor
Journal:  Can J Comp Med       Date:  1981-07

5.  Maturation of the head of bacteriophage T4. I. DNA packaging events.

Authors:  U K Laemmli; M Favre
Journal:  J Mol Biol       Date:  1973-11-15       Impact factor: 5.469

6.  Structural studies of the capsular polysaccharide from Haemophilus pleuropneumoniae serotype 1.

Authors:  E Altman; J R Brisson; M B Perry
Journal:  Biochem Cell Biol       Date:  1986-08       Impact factor: 3.626

7.  Characterization of Shigella flexneri-specific murine monoclonal antibodies by chemically defined glycoconjugates.

Authors:  N I Carlin; M A Gidney; A A Lindberg; D R Bundle
Journal:  J Immunol       Date:  1986-10-01       Impact factor: 5.422

8.  Serological confirmation of Brucella abortus and Yersinia enterocolitica O:9 O-antigens by monoclonal antibodies.

Authors:  D R Bundle; M A Gidney; M B Perry; J R Duncan; J W Cherwonogrodzky
Journal:  Infect Immun       Date:  1984-11       Impact factor: 3.441

9.  Immunochemistry of groups A, B, and C meningococcal polysaccharide-tetanus toxoid conjugates.

Authors:  H J Jennings; C Lugowski
Journal:  J Immunol       Date:  1981-09       Impact factor: 5.422

10.  High-molecular-weight components in lipopolysaccharides of Salmonella typhimurium, Salmonella minnesota, and Escherichia coli.

Authors:  A A Peterson; E J McGroarty
Journal:  J Bacteriol       Date:  1985-05       Impact factor: 3.490
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