Patrick Bellelis1, Denise Frediani Barbeiro2, Bárbara Yasmin Gueuvoghlanian-Silva3, Jorge Kalil4, Mauricio Simões Abrão5,6, Sergio Podgaec5,3. 1. Department of Obstetrics and Gynecology, University of São Paulo Medical School, São Paulo, Brazil, pbellelis@gmail.com. 2. Medical Investigation Laboratory (LIM 51), University of São Paulo Medical School, São Paulo, Brazil. 3. Jewish Teaching and Research Institute, Albert Einstein Hospital, São Paulo, Brazil. 4. INCT, Discipline of Allergy and Immunology (HC/Incor/FMUSP), São Paulo, Brazil. 5. Department of Obstetrics and Gynecology, University of São Paulo Medical School, São Paulo, Brazil. 6. Gynecologic Division, BP-A Beneficencia Portuguesa de Sao Paulo, São Paulo, Brazil.
Abstract
OBJECTIVE: The objective of this study was to evaluate cytokines related to natural killer and T-regulatory cells in endometriotic lesions, peritoneal fluid (PF) and the peripheral blood (PB) of patients with deep infiltrative endometriosis. STUDY DESIGN: A case-control study was conducted in a tertiary referral hospital. Sixty-four consecutive patients after laparoscopy were divided into 2 groups: with endometriosis (Group A - n = 32) and without endometriosis (Group B - n = 32). MAIN OUTCOME MEASURES: Interleukin (IL)-2, IL-4, IL-7, IL-10, IL-12, IL-15, transforming growth factor β1, and IFNγ concentration was measured using a LuminexTM multiplex suspension bead array. Tissues from endometriotic lesions of patients with endometriosis and from eutopic endometrium were evaluated, as well as PF and PB of all patients. RESULTS: Compared to the other analyzed groups, IL-15 concentration was significantly higher in the ectopic endometrium and IL-7 in the eutopic endometrium of the endometriosis group (p < 0.05). Compared to endometriosis group, IFNγ, IL-7, and IL-15 were observed to be significantly higher in the PF of the control group, and IL-10 was lower in the control group (p < 0.05). In PB, compared to endometriosis group, IL-4, IL-10, IL-12, IL-15, and IFNγ concentrations were significantly higher in the control group (p < 0.05). CONCLUSIONS: Our hypothesis is that deep endometriosis is a disease out of control. This disease's nature is of progression and invasion of adjacent structures, and proof of this disease state is the disorganized secretion of cytokine regulation and inflammation, which seem to be among the factors responsible for the maintenance of the disease.
OBJECTIVE: The objective of this study was to evaluate cytokines related to natural killer and T-regulatory cells in endometriotic lesions, peritoneal fluid (PF) and the peripheral blood (PB) of patients with deep infiltrative endometriosis. STUDY DESIGN: A case-control study was conducted in a tertiary referral hospital. Sixty-four consecutive patients after laparoscopy were divided into 2 groups: with endometriosis (Group A - n = 32) and without endometriosis (Group B - n = 32). MAIN OUTCOME MEASURES: Interleukin (IL)-2, IL-4, IL-7, IL-10, IL-12, IL-15, transforming growth factor β1, and IFNγ concentration was measured using a LuminexTM multiplex suspension bead array. Tissues from endometriotic lesions of patients with endometriosis and from eutopic endometrium were evaluated, as well as PF and PB of all patients. RESULTS: Compared to the other analyzed groups, IL-15 concentration was significantly higher in the ectopic endometrium and IL-7 in the eutopic endometrium of the endometriosis group (p < 0.05). Compared to endometriosis group, IFNγ, IL-7, and IL-15 were observed to be significantly higher in the PF of the control group, and IL-10 was lower in the control group (p < 0.05). In PB, compared to endometriosis group, IL-4, IL-10, IL-12, IL-15, and IFNγ concentrations were significantly higher in the control group (p < 0.05). CONCLUSIONS: Our hypothesis is that deep endometriosis is a disease out of control. This disease's nature is of progression and invasion of adjacent structures, and proof of this disease state is the disorganized secretion of cytokine regulation and inflammation, which seem to be among the factors responsible for the maintenance of the disease.
Authors: Lyudmila M Mikhaleva; Victor E Radzinsky; Mekan R Orazov; Tatyana N Khovanskaya; Anastasia V Sorokina; Sergey A Mikhalev; Snezhana V Volkova; Victoria B Shustova; Mikhail Y Sinelnikov Journal: Int J Womens Health Date: 2021-06-01
Authors: Helena Malvezzi; Cristine Dobo; Renee Zon Filippi; Helen Mendes do Nascimento; Laura Palmieri da Silva E Sousa; Juliana Meola; Carla Azevedo Piccinato; Sérgio Podgaec Journal: Int J Mol Sci Date: 2022-02-24 Impact factor: 5.923
Authors: Camila Hernandes; Renata Nascimento de Oliveira; Artur Henrique de Souza Santos; Helena Malvezzi; Bruna Cestari de Azevedo; Bárbara Yasmin Gueuvoghlanian-Silva; Ana Maria Soares Pereira; Sergio Podgaec Journal: Molecules Date: 2020-03-13 Impact factor: 4.411