Literature DB >> 30706993

Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes.

Guillaume Adelmant1,2, Brijesh K Garg1, Maria Tavares1, Joseph D Card1, Jarrod A Marto1,2.   

Abstract

Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP) primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure.
© 2019 by John Wiley & Sons, Inc. © 2019 John Wiley & Sons, Inc.

Entities:  

Keywords:  TAP; affinity purification; epitope tags; mass spectrometry; protein complex; protein-protein interaction

Mesh：

Substances：

Year:  2019        PMID： 30706993      PMCID： PMC6579647          DOI： 10.1002/cpps.84

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  64 in total

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3.  CRKL Mediates p110β-Dependent PI3K Signaling in PTEN-Deficient Cancer Cells.

Authors:  Jing Zhang; Xueliang Gao; Fabienne Schmit; Guillaume Adelmant; Michael J Eck; Jarrod A Marto; Jean J Zhao; Thomas M Roberts
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4.  Peptidic degron in EID1 is recognized by an SCF E3 ligase complex containing the orphan F-box protein FBXO21.

Authors:  Cuiyan Zhang; Xiaotong Li; Guillaume Adelmant; Jessica Dobbins; Christoph Geisen; Matthew G Oser; Kai W Wucherpfenning; Jarrod A Marto; William G Kaelin
Journal:  Proc Natl Acad Sci U S A       Date:  2015-12-02       Impact factor: 11.205

5.  Structural Basis for Substrate Selectivity of the E3 Ligase COP1.

Authors:  Sacha Uljon; Xiang Xu; Izabela Durzynska; Sarah Stein; Guillaume Adelmant; Jarrod A Marto; Warren S Pear; Stephen C Blacklow
Journal:  Structure       Date:  2016-03-31       Impact factor: 5.006

6.  Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation.

Authors:  Laure Ferry; Alexandra Fournier; Takeshi Tsusaka; Guillaume Adelmant; Tadahiro Shimazu; Shohei Matano; Olivier Kirsh; Rachel Amouroux; Naoshi Dohmae; Takehiro Suzuki; Guillaume J Filion; Wen Deng; Maud de Dieuleveult; Lauriane Fritsch; Srikanth Kudithipudi; Albert Jeltsch; Heinrich Leonhardt; Petra Hajkova; Jarrod A Marto; Kyohei Arita; Yoichi Shinkai; Pierre-Antoine Defossez
Journal:  Mol Cell       Date:  2017-08-10       Impact factor: 17.970

7.  Concordant and opposite roles of DNA-PK and the "facilitator of chromatin transcription" (FACT) in DNA repair, apoptosis and necrosis after cisplatin.

Authors:  Janna Sand-Dejmek; Guillaume Adelmant; Bijan Sobhian; Anne S Calkins; Jarrod Marto; Dirk J Iglehart; Jean-Bernard Lazaro
Journal:  Mol Cancer       Date:  2011-06-16       Impact factor: 27.401

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Authors:  Roberto Giambruno; Florian Grebien; Alexey Stukalov; Christian Knoll; Melanie Planyavsky; Elena L Rudashevskaya; Jacques Colinge; Giulio Superti-Furga; Keiryn L Bennett
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9.  Proteomic analysis reveals CACN-1 is a component of the spliceosome in Caenorhabditis elegans.

Authors:  Michael F Doherty; Guillaume Adelmant; Alyssa D Cecchetelli; Jarrod A Marto; Erin J Cram
Journal:  G3 (Bethesda)       Date:  2014-06-19       Impact factor: 3.154

10.  The chromatin remodeling factor CHD5 is a transcriptional repressor of WEE1.

Authors:  Jinhua Quan; Guillaume Adelmant; Jarrod A Marto; A Thomas Look; Timur Yusufzai
Journal:  PLoS One       Date:  2014-09-23       Impact factor: 3.240
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  7 in total

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2.  STRIPAK directs PP2A activity toward MAP4K4 to promote oncogenic transformation of human cells.

Authors:  Jong Wook Kim; Christian Berrios; Miju Kim; Amy E Schade; Guillaume Adelmant; Huwate Yeerna; Emily Damato; Amanda Balboni Iniguez; Laurence Florens; Michael P Washburn; Kim Stegmaier; Nathanael S Gray; Pablo Tamayo; Ole Gjoerup; Jarrod A Marto; James DeCaprio; William C Hahn
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Review 3.  Protein Complexes Form a Basis for Complex Hybrid Incompatibility.

Authors:  Krishna B S Swamy; Scott C Schuyler; Jun-Yi Leu
Journal:  Front Genet       Date:  2021-02-09       Impact factor: 4.599

4.  Determining the Effects of Neddylation on Cullin-RING Ligase-Dependent Protein Ubiquitination.

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Review 5.  Uncovering Novel Viral Innate Immune Evasion Strategies: What Has SARS-CoV-2 Taught Us?

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6.  IER5, a DNA damage response gene, is required for Notch-mediated induction of squamous cell differentiation.

Authors:  Li Pan; Madeleine E Lemieux; Tom Thomas; Julia M Rogers; Colin H Lipper; Winston Lee; Carl Johnson; Lynette M Sholl; Andrew P South; Jarrod A Marto; Guillaume O Adelmant; Stephen C Blacklow; Jon C Aster
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Review 7.  Prospects and challenges of cancer systems medicine: from genes to disease networks.

Authors:  Mohammad Reza Karimi; Amir Hossein Karimi; Shamsozoha Abolmaali; Mehdi Sadeghi; Ulf Schmitz
Journal:  Brief Bioinform       Date:  2022-01-17       Impact factor: 11.622
  7 in total

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