Mayank Verma1, Yoko Asakura2, Atsushi Asakura3. 1. Medical Scientist Training Program, University of Minnesota Medical School, Minneapolis, Minnesota, USA. 2. Stem Cell Institute, University of Minnesota Medical School, Minneapolis, Minnesota, USA. 3. Department of Neurology, University of Minnesota Medical School, Minneapolis, Minnesota, 55455, USA.
Abstract
INTRODUCTION: The vasculature and blood flow in muscle are perturbed in Duchenne muscular dystrophy (DMD) and its mdx mouse model. MicroRNA-92a (miR-92a) is enriched in endothelial cells, especially during ischemic injury. METHODS: Because antagonizing miR-92a was shown to result in increased proliferation and migration of endothelial cells and recovery from ischemia, we assessed the effects of Antagomir-92a in vitro in muscle stem cell culture and in vivo in mdx mice. RESULTS: miR-92a was found to be highly expressed in muscle endothelial cells and satellite cells. Treatment with Antagomir-92a increased capillary density and tissue perfusion, which was accompanied by an increase in satellite cells. However, Antagomir-92a-treated mdx mice showed no histological improvement and had worse muscle function. Antagomir-92a suppressed myogenic differentiation in satellite cell culture. DISCUSSION: AntagomiR-92a improves the vasculature but not the muscle in mdx mice, possibly due to its side effects on satellite cell differentiation. Muscle Nerve 59:594-594, 2019.
INTRODUCTION: The vasculature and blood flow in muscle are perturbed in Duchenne muscular dystrophy (DMD) and its mdx mouse model. MicroRNA-92a (miR-92a) is enriched in endothelial cells, especially during ischemic injury. METHODS: Because antagonizing miR-92a was shown to result in increased proliferation and migration of endothelial cells and recovery from ischemia, we assessed the effects of Antagomir-92a in vitro in muscle stem cell culture and in vivo in mdx mice. RESULTS: miR-92a was found to be highly expressed in muscle endothelial cells and satellite cells. Treatment with Antagomir-92a increased capillary density and tissue perfusion, which was accompanied by an increase in satellite cells. However, Antagomir-92a-treated mdx mice showed no histological improvement and had worse muscle function. Antagomir-92a suppressed myogenic differentiation in satellite cell culture. DISCUSSION: AntagomiR-92a improves the vasculature but not the muscle in mdx mice, possibly due to its side effects on satellite cell differentiation. Muscle Nerve 59:594-594, 2019.
Authors: Alessandra Sacco; Foteini Mourkioti; Rose Tran; Jinkuk Choi; Michael Llewellyn; Peggy Kraft; Marina Shkreli; Scott Delp; Jason H Pomerantz; Steven E Artandi; Helen M Blau Journal: Cell Date: 2010-12-09 Impact factor: 41.582
Authors: Arun Richard Chandrasekaran; Jibin Abraham Punnoose; Lifeng Zhou; Paromita Dey; Bijan K Dey; Ken Halvorsen Journal: Nucleic Acids Res Date: 2019-11-18 Impact factor: 16.971