| Literature DB >> 30692678 |
Paula Oyarce1,2, Barbara De Meester1,2, Fernando Fonseca1,2, Lisanne de Vries1,2, Geert Goeminne1,2,3, Andreas Pallidis1,2, Riet De Rycke1,2,3,4, Yukiko Tsuji5,6, Yanding Li5,6, Sander Van den Bosch7, Bert Sels7, John Ralph5,6, Ruben Vanholme1,2,3, Wout Boerjan8,9,10.
Abstract
Lignin is the main cause of lignocellulosic biomass recalcitrance to industrial enzymatic hydrolysis. By partially replacing the traditional lignin monomers by alternative ones, lignin extractability can be enhanced. To design a lignin that is easier to degrade under alkaline conditions, curcumin (diferuloylmethane) was produced in the model plant Arabidopsis thaliana via simultaneous expression of the turmeric (Curcuma longa) genes DIKETIDE-CoA SYNTHASE (DCS) and CURCUMIN SYNTHASE 2 (CURS2). The transgenic plants produced a plethora of curcumin- and phenylpentanoid-derived compounds with no negative impact on growth. Catalytic hydrogenolysis gave evidence that both curcumin and phenylpentanoids were incorporated into the lignifying cell wall, thereby significantly increasing saccharification efficiency after alkaline pretreatment of the transgenic lines by 14-24% as compared with the wild type. These results demonstrate that non-native monomers can be synthesized and incorporated into the lignin polymer in plants to enhance their biomass processing efficiency.Entities:
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Year: 2019 PMID： 30692678 DOI： 10.1038/s41477-018-0350-3
Source DB: PubMed Journal: Nat Plants ISSN： 2055-0278 Impact factor: 15.793