| Literature DB >> 30674007 |
Maria S Mazanko1, Maksim S Makarenko2, Vladimir A Chistyakov1, Alexander V Usatov1, Evgeniya V Prazdnova1, Anzhelika B Bren1, Ivan F Gorlov3, Zoya B Komarova3, Richard Weeks4, Michael L Chikindas1,4.
Abstract
A promising approach for slowing down the rate of reproductive aging is the use of probiotic bacteria as a feed additive. In the current study was investigated the influence of the intake of a potential probiotic on the follicle content and expression of vitellogenin genes (vtg1, vtg2, vtg3) in aged hens. RNA was isolated from liver samples collected from 570-day-old laying hens and gene expression levels were measured using RT-PCR. Bacillus subtilis KATMIRA1933 supplementation had a positive effect on the number of formed follicles in hens and also triggered a significant increase in the relative expression levels of vtg1, vtg2, and vtg3. A Bacillus amyloliquefaciens B-1895 enriched diet or a combination of the two strains had a modest effect on both the number of follicles and the expression of vitellogenin genes. Additionally, the study demonstrates that vitellogenin mRNA expression levels can be considered as a biomarker in a convenient approach for analyzing the hen's egg-laying ability.Entities:
Keywords: Bacillus; Poultry; Probiotic supplementation; Reproductive aging; Vitellogenin; vtg
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Year: 2019 PMID： 30674007 DOI： 10.1007/s12602-019-9519-y
Source DB: PubMed Journal: Probiotics Antimicrob Proteins ISSN： 1867-1306 Impact factor: 4.609