| Literature DB >> 30670663 |
Andreas Geissner1,2, Anika Reinhardt1,2, Christoph Rademacher1,2, Timo Johannssen1,2, João Monteiro3, Bernd Lepenies1,2,3, Michel Thépaut4,5,6, Franck Fieschi4,5,6, Jana Mrázková7, Michaela Wimmerova7, Frank Schuhmacher1,2, Sebastian Götze1,2, Dan Grünstein1,2, Xiaoqiang Guo1,2, Heung Sik Hahm1,2, Jeyakumar Kandasamy1, Daniele Leonori1, Christopher E Martin1,2, Sharavathi G Parameswarappa1, Sandip Pasari1, Mark K Schlegel1, Hidenori Tanaka1, Guozhi Xiao1, You Yang1, Claney L Pereira1, Chakkumkal Anish1, Peter H Seeberger8,2.
Abstract
Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.Entities:
Keywords: antiglycan antibodies; bacterial lectins; glycan arrays; immune receptors; microbial antigens
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Year: 2019 PMID： 30670663 PMCID： PMC6369816 DOI： 10.1073/pnas.1800853116
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205