Huili Zhang1, Xinyu Zhao2, Chongjian Wang3, Ran Du1, Xiaojie Wang1, Jitong Fu1, Qi Sun1. 1. Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. 2. Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. Electronic address: cindy_zhaoxinyu@163.com. 3. Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, China.
Abstract
AIM: To investigate association of Apolipoprotein E (ApoE) gene promoter methylation with atherosclerotic cerebral infarction (ACI) in the Han Chinese population. METHODS: Twenty-six ACI patients (the case group) and 26 healthy (the control group) were recruited randomly from Henan Han nationality population, from April 2016 to August 2016. Bisulfite pyrosequencing technology was used to examine the role of the aberrant gene promoter methylation in ACI in Han Chinese population. Especially, we used the odds ratio and 95% confidence interval (95% CI) method to elevate the correlation between ApoE Promoter Methylation and ACI. RESULTS: The case group was significantly more likely to have hypertension and carotid atherosclerotic plaques (Table 1). The case group had significantly lower levels of high-density lipoprotein cholesterol (HDL-C), folate, and higher levels of homocysteine (Table 2). We observed that ACI patients (n = 26) had significantly higher methylation levels at cytosine-phosphate-guanine (CpG) 14 and CpG16 compared with controls (n = 26) (Table 3). Importantly, our results indicated a significant association between ApoE promoter methylation and ACI (OR = 16.146; 95% CI, 1.154-225.832; P* < .05; P*: adjusted for age, gender, carotid atherosclerotic plaque, hypertension, HDL-C, homocysteine, and folate) (Table 4). CONCLUSIONS: Our study indicates that ApoE promoter methylation may be associated with ACI in Han Chinese people.
AIM: To investigate association of Apolipoprotein E (ApoE) gene promoter methylation with atherosclerotic cerebral infarction (ACI) in the Han Chinese population. METHODS: Twenty-six ACI patients (the case group) and 26 healthy (the control group) were recruited randomly from Henan Han nationality population, from April 2016 to August 2016. Bisulfite pyrosequencing technology was used to examine the role of the aberrant gene promoter methylation in ACI in Han Chinese population. Especially, we used the odds ratio and 95% confidence interval (95% CI) method to elevate the correlation between ApoE Promoter Methylation and ACI. RESULTS: The case group was significantly more likely to have hypertension and carotid atherosclerotic plaques (Table 1). The case group had significantly lower levels of high-density lipoprotein cholesterol (HDL-C), folate, and higher levels of homocysteine (Table 2). We observed that ACI patients (n = 26) had significantly higher methylation levels at cytosine-phosphate-guanine (CpG) 14 and CpG16 compared with controls (n = 26) (Table 3). Importantly, our results indicated a significant association between ApoE promoter methylation and ACI (OR = 16.146; 95% CI, 1.154-225.832; P* < .05; P*: adjusted for age, gender, carotid atherosclerotic plaque, hypertension, HDL-C, homocysteine, and folate) (Table 4). CONCLUSIONS: Our study indicates that ApoE promoter methylation may be associated with ACI in Han Chinese people.
Authors: Ni Putu Tesi Maratni; Made Ratna Saraswati; Ni Nyoman Ayu Dewi; I Wayan Putu Sutirta Yasa; I Putu Eka Widyadharma; Ida Bagus Kusuma Putra; Ketut Suastika Journal: J Nutr Metab Date: 2021-03-26
Authors: Esbeidy García-Flores; José Manuel Rodríguez-Pérez; Verónica Marusa Borgonio-Cuadra; Gilberto Vargas-Alarcón; Juan Calderón-Colmenero; Juan Pablo Sandoval; José Antonio García-Montes; Víctor Manuel Espinoza-Gutiérrez; Juan Gerardo Reyes-García; Benny Giovanni Cazarín-Santos; Antonio Miranda-Duarte; Armando Gamboa-Domínguez; Nonanzit Pérez-Hernández Journal: Biology (Basel) Date: 2022-01-08