Razvan Opris1, Vlad Toma2,3,4, Diana Olteanu1, Ioana Baldea1, Alina Mihaela Baciu1, Florica Imre Lucaci5, Alexandra Berghian-Sevastre1, Corina Tatomir6, Bianca Moldovan7, Simona Clichici1, Luminita David7, Adrian Florea8, Gabriela Adriana Filip1. 1. Department of Physiology, 'Iuliu Hatieganu' University of Medicine & Pharmacy, 1-3 Clinicilor Street, 400006, Cluj-Napoca, Romania. 2. Department of Molecular Biology & Biotechnology, Faculty of Biology & Geology, 'Babes-Bolyai' University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania. 3. Department of Biochemistry & Experimental Biology, Institute of Biological Research, 48 Republicii Street, branch of NIRDBS Bucharest, 400015 Cluj-Napoca, Romania. 4. Department of Molecular & Biomolecular Physics, NIRD for Isotopic & Molecular Technologies, 101 Donath Street, 400293 Cluj-Napoca, Romania. 5. Physico-Chemical Analysis Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, 'Babes-Bolyai' University, 42 Treboniu Laurian Street, 400271 Cluj-Napoca, Romania. 6. Departments of Radiobiology & Tumour Biology, 'Ion Chiricuta' Oncology Institute, 34-36 Republicii Street, 400015, Cluj-Napoca, Romania. 7. Department of Chemistry, Faculty of Chemistry & Chemical Engineering, 'Babes-Bolyai' University, 11. Arany Janos, 400028 Cluj-Napoca, Romania. 8. Department of Cell & Molecular Biology, 'Iuliu Hatieganu' University of Medicine & Pharmacy 6, Louis Pasteur Street, 400349, Cluj Napoca, Romania.
Abstract
AIM: To assess ultrastructural changes, alterations in matrix metalloproteinase activity and apoptosis induced by silver nanoparticles (AgNPs) in the rat testicle. MATERIALS & METHODS: For 45 days, two groups of animals received different doses of AgNPs (0.8 and 1.5 mg/kg b.w.), and a control group was given the buffer used as vehicle for AgNPs. At 7 and 15 days post-treatment, transmission electron microscopy, TUNEL assay, evaluation of NFkB, pNFkB, p53, Bcl-2 and Nrf2 expressions were performed on the removed testes. RESULTS: Transmission electron microscopy revealed severe ultrastructural changes of interstitial tissue and seminiferous epithelium sustained by positive signal for apoptosis. The promatrix metalloproteinase-2 activity and NFkB, Bcl-2 expressions were increased, mainly at 7 days. CONCLUSION: AgNPs induced severe cell lesions identified even a long time after the exposure.
AIM: To assess ultrastructural changes, alterations in matrix metalloproteinase activity and apoptosis induced by silver nanoparticles (AgNPs) in the rat testicle. MATERIALS & METHODS: For 45 days, two groups of animals received different doses of AgNPs (0.8 and 1.5 mg/kg b.w.), and a control group was given the buffer used as vehicle for AgNPs. At 7 and 15 days post-treatment, transmission electron microscopy, TUNEL assay, evaluation of NFkB, pNFkB, p53, Bcl-2 and Nrf2 expressions were performed on the removed testes. RESULTS: Transmission electron microscopy revealed severe ultrastructural changes of interstitial tissue and seminiferous epithelium sustained by positive signal for apoptosis. The promatrix metalloproteinase-2 activity and NFkB, Bcl-2 expressions were increased, mainly at 7 days. CONCLUSION: AgNPs induced severe cell lesions identified even a long time after the exposure.
Entities:
Keywords:
apoptosis; electron microscopy; rat testicle; silver nanoparticles; transcription factors
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