Literature DB >> 3065448

Purification and characterization of a 36 kDa antigen of Mycobacterium leprae.

M Y de Wit1, P R Klatser.   

Abstract

A 36 kDa antigen of Mycobacterium leprae was purified by phenol biphasic partition followed by preparative SDS-PAGE. The purified antigen appeared as a single band in SDS-PAGE and eluted as a single peak in ion-exchange chromatography. The antigen comprised epitopes which were cross-reactive with M. tuberculosis, as well as a species-specific epitope (recognized by MAb F47-9). Different treatments of the 36 kDa antigen suggested it to be largely protein in nature; the amino acid composition of 81% of the antigen was determined. A majority of sera from leprosy patients contained antibodies recognizing the 36 kDa antigen.

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Year:  1988        PMID： 3065448     DOI： 10.1099/00221287-134-6-1541

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  6 in total

1.  Enzyme-linked immunosorbent assay for distinguishing serological responses of lepromatous and tuberculoid leprosies to the 29/33-kilodalton doublet and 64-kilodalton antigens of Mycobacterium tuberculosis.

Authors:  P K Das; A Rambukkana; J G Baas; D G Groothuis; M Halperin
Journal:  J Clin Microbiol       Date:  1990-02       Impact factor: 5.948

2.  Monoclonal immunoglobulin G1 directed against Aspergillus fumigatus cell wall glycoprotein protects against experimental murine aspergillosis.

Authors:  Ashok K Chaturvedi; A Kavishwar; G B Shiva Keshava; P K Shukla
Journal:  Clin Diagn Lab Immunol       Date:  2005-09

3.  A major immunogenic 36,000-molecular-weight antigen from Mycobacterium leprae contains an immunoreactive region of proline-rich repeats.

Authors:  J E Thole; L F Stabel; M E Suykerbuyk; M Y De Wit; P R Klatser; A H Kolk; R A Hartskeerl
Journal:  Infect Immun       Date:  1990-01       Impact factor: 3.441

4.  Characterization of murine B-cell epitopes on the Mycobacterium leprae proline-rich antigen by use of synthetic peptides.

Authors:  P R Klatser; M Y De Wit; A H Kolk; R A Hartskeerl
Journal:  Infect Immun       Date:  1991-01       Impact factor: 3.441

5.  Production and characterization of monoclonal antibodies to a 58-kilodalton antigen of Aspergillus fumigatus.

Authors:  P M Fratamico; W K Long; H R Buckley
Journal:  Infect Immun       Date:  1991-01       Impact factor: 3.441

6.  Chemical definition, cloning, and expression of the major protein of the leprosy bacillus.

Authors:  B Rivoire; M C Pessolani; C M Bozic; S W Hunter; S A Hefta; V Mehra; P J Brennan
Journal:  Infect Immun       Date:  1994-06       Impact factor: 3.441
  6 in total

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