Literature DB >> 2107205

Enzyme-linked immunosorbent assay for distinguishing serological responses of lepromatous and tuberculoid leprosies to the 29/33-kilodalton doublet and 64-kilodalton antigens of Mycobacterium tuberculosis.

P K Das1, A Rambukkana, J G Baas, D G Groothuis, M Halperin.   

Abstract

Immunoblot assays for the antibodies to Mycobacterium tuberculosis sonic extracts showed that all serum specimens of 40 lepromatous and of 28 tuberculoid leprosy patients reacted in a significant manner to 29/33-kilodalton (kDa) doublet and 64-kDa antigens, respectively. By using an enzyme-linked immunosorbent assay, we observed a significantly high immunoglobulin G antibody titer to the purified M. tuberculosis 29/33-kDa doublet and 64-kDa antigens in lepromatous and tuberculoid leprosy patients, respectively, as compared with normal subjects and tuberculosis patients. This enzyme-linked immunosorbent assay serology may be useful for distinguishing two polar types of leprosy and for diagnosing leprosy in general.

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Year:  1990        PMID： 2107205      PMCID： PMC269615          DOI： 10.1128/jcm.28.2.379-382.1990

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

Review 1.  Mycobacteria and autoimmunity.

Authors:  Y Shoenfeld; D A Isenberg
Journal:  Immunol Today       Date:  1988-06

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Immunological aspects of leprosy: ten years' activity at the Armauer Hansen Research Institute and Prospects for further work.

Authors:  M Harboe
Journal:  Int J Lepr Other Mycobact Dis       Date:  1980-06

4.  A serological test for leprosy based on competitive inhibition of monoclonal antibody binding to the MY2a determinant of Mycobacterium leprae.

Authors:  S Sinha; U Sengupta; G Ramu; J Ivanyi
Journal:  Trans R Soc Trop Med Hyg       Date:  1983       Impact factor: 2.184

5.  Definition of species specific and cross-reactive antigenic determinants of Mycobacterium leprae using monoclonal antibodies.

Authors:  J Ivanyi; S Sinha; R Aston; D Cussell; M Keen; U Sengupta
Journal:  Clin Exp Immunol       Date:  1983-06       Impact factor: 4.330

Review 6.  Leprosy and tuberculosis--an epidemiological comparison.

Authors:  P Fine
Journal:  Tubercle       Date:  1984-06

7.  The use of subcellular components of BCG for studying host-Mycobacterium interaction in relation to leprosy.

Authors:  P K Das; A A Ibrahim; F Binkhuysen; P Thi Hué; T H Qui; R Rangarajan; E J Ruitenberg
Journal:  Ann Immunol (Paris)       Date:  1982 Jul-Aug

8.  Leprosy research, present and future.

Authors:  J L Stanford
Journal:  Acta Leprol       Date:  1984 Oct-Dec

9.  Immunochemical characterization of Mycobacterium leprae antigens by the SDS-polyacrylamide gel electrophoresis immunoperoxidase technique (SGIP) using patients' sera.

Authors:  P R Klatser; M M van Rens; T A Eggelte
Journal:  Clin Exp Immunol       Date:  1984-06       Impact factor: 4.330

10.  Use of a unit gravity sedimentation chamber for the purification of Mycobacterium leprae.

Authors:  P K Das; A Tulp
Journal:  Ann Microbiol (Paris)       Date:  1982 Nov-Dec
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  14 in total

1.  Humoral responses against the 85A and 85B antigens of Mycobacterium bovis BCG in patients with leprosy and tuberculosis.

Authors:  J P Van Vooren; A Drowart; J De Bruyn; P Launois; J Millan; E Delaporte; M Develoux; J C Yernault; K Huygen
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

2.  Effect of intestinal resection on serum antibodies to the mycobacterial 45/48 kilodalton doublet antigen in Crohn's disease.

Authors:  G Kreuzpaintner; P K Das; A Stronkhorst; A W Slob; G Strohmeyer
Journal:  Gut       Date:  1995-09       Impact factor: 23.059

3.  Mycobacterium tuberculosis H(37)Ra ESAS-7-An excretory-secretory antigen fraction of immunodiagnostic potential in pulmonary tuberculosis.

Authors:  E R Nair; S Kumar; M V Reddy; B C Harinath
Journal:  Indian J Clin Biochem       Date:  1998-07

4.  An immunodominant 30-kDa antigen of a candidate anti-leprosy vaccine, Mycobacterium w, shares T and B cell determinants with M. leprae and M. tuberculosis.

Authors:  A Yadava; R Mukherjee
Journal:  Med Microbiol Immunol       Date:  1993-11       Impact factor: 3.402

5.  The mycobacterial secreted antigen 85 complex possesses epitopes that are differentially expressed in human leprosy lesions and Mycobacterium leprae-infected armadillo tissues.

Authors:  A Rambukkana; J D Burggraaf; W R Faber; M Harboe; P Teeling; S Krieg; P K Das
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441

6.  T cell response to purified filtrate antigen 85 from Mycobacterium bovis Bacilli Calmette-Guérin (BCG) in leprosy patients.

Authors:  P Launois; K Huygen; J De Bruyn; M N'Diaye; B Diouf; L Sarthouj; J Grimaud; J Millan
Journal:  Clin Exp Immunol       Date:  1991-11       Impact factor: 4.330

7.  Serology and leprosy: immunoassays comparing immunoglobulin G antibody responses to 28- and 30-kilodalton proteins purified from Mycobacterium bovis BCG.

Authors:  M C Pessolani; J M Peralta; F D Rumjanek; H M Gomes; M A Marques; E C Almeida; M H Saad; E N Sarno
Journal:  J Clin Microbiol       Date:  1991-10       Impact factor: 5.948

8.  Identification and characterization of epitopes shared between the mycobacterial 65-kilodalton heat shock protein and the actively secreted antigen 85 complex: their in situ expression on the cell wall surface of Mycobacterium leprae.

Authors:  A Rambukkana; P K Das; J D Burggraaf; W R Faber; P Teeling; S Krieg; J E Thole; M Harboe
Journal:  Infect Immun       Date:  1992-11       Impact factor: 3.441

9.  Mycobacterial 65,000 MW heat-shock protein shares a carboxy-terminal epitope with human epidermal cytokeratin 1/2.

Authors:  A Rambukkana; P K Das; S Krieg; S Young; I C Le Poole; J D Bos
Journal:  Immunology       Date:  1992-10       Impact factor: 7.397

10.  Heterogeneity of monoclonal antibody-reactive epitopes on mycobacterial 30-kilodalton-region proteins and the secreted antigen 85 complex and demonstration of antigen 85B on the Mycobacterium leprae cell wall surface.

Authors:  A Rambukkana; P K Das; J D Burggraaf; S Yong; W R Faber; J E Thole; M Harboe
Journal:  Infect Immun       Date:  1992-12       Impact factor: 3.441
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