Literature DB >> 3064780

Underestimation of D-glucose phosphorylation as measured by 3H2O production from D-[2-3H]glucose.

W J Malaisse1, M T Yilmaz, F Malaisse-Lagae, A Sener.   

Abstract

In rat pancreatic islets, tumoral islet cells (RINm5F line), parotid gland, and in human erythrocytes, but not in rat hepatocytes, the production of 3H2O from D-[2-3H]glucose is 20-30% lower than from D-[5-3H]glucose. This coincides with the production of tritiated lactic acid from D-[2-3H]glucose and may be attributable to an intramolecular hydrogen transfer in the phosphoglucoisomerase reaction. It is concluded that the production of 3H2O from D-[2-3H]glucose is not a reliable tool to assess the total rate of hexose phosphorylation.

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Year:  1988        PMID： 3064780     DOI： 10.1016/0885-4505(88)90101-6

Source DB:  PubMed          Journal:  Biochem Med Metab Biol        ISSN： 0885-4505


  6 in total

1.  D-glucose metabolism in normal dispersed islet cells and tumoral INS-1 cells.

Authors:  A B Nadi; E Olivares; W J Malaisse
Journal:  Mol Cell Biochem       Date:  2000-07       Impact factor: 3.396

2.  Menadione- and cyclic AMP-induced alteration of the ratio between D-[2-3H]glucose detritiation and phosphorylation in rat erythrocytes.

Authors:  B Manuel y Keenoy; W J Malaisse
Journal:  Mol Cell Biochem       Date:  1993-04-07       Impact factor: 3.396

3.  Hexose metabolism in pancreatic islets: the glucose-6-phosphatase riddle.

Authors:  M A Perales; A Sener; W J Malaisse
Journal:  Mol Cell Biochem       Date:  1991-02-27       Impact factor: 3.396

4.  Dissociated effects of 2-deoxy-D-glucose on D-[2-3H]glucose and D-[5-3H]glucose conversion into 3HOH in rat erythrocytes.

Authors:  B Manuel y Keenoy; D Zähner; W J Malaisse
Journal:  Biochem J       Date:  1992-12-01       Impact factor: 3.857

5.  Phosphoglucoisomerase-catalyzed interconversion of hexose-phosphates. Isotopic discrimination between hydrogen and tritium.

Authors:  F Malaisse-Lagae; V Liemans; W J Malaisse
Journal:  Mol Cell Biochem       Date:  1989-08-15       Impact factor: 3.396

6.  Metabolism of tritiated D-glucose anomers in rat erythrocytes.

Authors:  Y Zhang; H Jijakli; P Courtois; A Sener; W J Malaisse
Journal:  Mol Cell Biochem       Date:  2004-04       Impact factor: 3.396
  6 in total

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