Wen-Chao Li1, Kai Wang1, You-Fang Gu2. 1. College of Animal Science, Anhui Science and Technology University, No. 9 Road Donghua, Fengyang, 233100, Anhui Province, People's Republic of China. 2. College of Animal Science, Anhui Science and Technology University, No. 9 Road Donghua, Fengyang, 233100, Anhui Province, People's Republic of China. youfanggu@163.com.
Abstract
BACKGROUND: Enterocytozoon bieneusi, the dominant zoonotic species of microsporidia, has been reported in various animals and humans. However, relatively little information is available concerning the worldwide prevalence and genotypes of E. bieneusi in ruminants, such as sheep and goats. The present study was conducted to evaluate the prevalence and genetic characteristics of E. bieneusi in sheep and goats in east-central China. METHODS: Fresh fecal samples from 832 sheep and 781 goats were evaluated for the presence of E. bieneusi using PCR and sequencing of the ribosomal internal transcribed spacer. RESULTS: 28 sheep specimens (3.4%) and 32 goat specimens (4.1%) were positive for E. bieneusi. A total of 12 ITS genotypes were identified, including 7 known genotypes (COS-I, OEB1, BEB6, CHG1, CHG3, COS-II, and CHC8) and 5 novel genotypes (named AHS1, AHS2, JSS1, AHG1, and AHG2). The dominant genotypes in sheep and goats were BEB6 and CHG3, respectively. Phylogenetic analysis revealed that all genotypes obtained in this study belonged to the so-called "cattle-specific" Group 2. CONCLUSIONS: These findings show a relatively low occurrence and genetic diversity of E. bieneusi in sheep and goats in east-central China. Further studies are required to elucidate the role of sheep and goats in the epidemiology of microsporidia.
BACKGROUND: Enterocytozoon bieneusi, the dominant zoonotic species of microsporidia, has been reported in various animals and humans. However, relatively little information is available concerning the worldwide prevalence and genotypes of E. bieneusi in ruminants, such as sheep and goats. The present study was conducted to evaluate the prevalence and genetic characteristics of E. bieneusi in sheep and goats in east-central China. METHODS: Fresh fecal samples from 832 sheep and 781 goats were evaluated for the presence of E. bieneusi using PCR and sequencing of the ribosomal internal transcribed spacer. RESULTS: 28 sheep specimens (3.4%) and 32 goat specimens (4.1%) were positive for E. bieneusi. A total of 12 ITS genotypes were identified, including 7 known genotypes (COS-I, OEB1, BEB6, CHG1, CHG3, COS-II, and CHC8) and 5 novel genotypes (named AHS1, AHS2, JSS1, AHG1, and AHG2). The dominant genotypes in sheep and goats were BEB6 and CHG3, respectively. Phylogenetic analysis revealed that all genotypes obtained in this study belonged to the so-called "cattle-specific" Group 2. CONCLUSIONS: These findings show a relatively low occurrence and genetic diversity of E. bieneusi in sheep and goats in east-central China. Further studies are required to elucidate the role of sheep and goats in the epidemiology of microsporidia.
Authors: Vagner Ricardo da Silva Fiuza; Carlos Wilson Gomes Lopes; Rachel Ingrid Juliboni Cosendey; Francisco Carlos Rodrigues de Oliveira; Ronald Fayer; Monica Santín Journal: Res Vet Sci Date: 2016-06-07 Impact factor: 2.534