Xing-Yu Feng1,2,3, Lin Lu4,5, Ke-Feng Wang1,3, Bao-Yan Zhu1,3, Xi-Zhi Wen1,3, Rui-Qing Peng1,3, Ya Ding1,3, Dan-Dan Li1,3, Jing-Jing Li1,3, Yong Li2, Xiao-Shi Zhang1,3. 1. Biotherapy Center, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, PR China. 2. Department of General Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences, School of Medicine, South China University of Technology. 106 Zhongshan Road 2, Guangzhou 510080, PR China. 3. State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou 510060, PR China. 4. Department of Medical Oncology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou 510180, PR China. 5. Department of Medical Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, 602 Renminbei Road, Guangzhou 510180, PR China.
Abstract
AIM: To study the expression and prognostic significance of CD80 in patients with gastric adenocarcinoma. Materials & methods: Real-time quantitative PCR, western blot and immunohistochemistry were performed to detect the expression of CD80 in gastric cancer tissues and matched adjacent normal tissues. Double immunohistochemical staining was performed to preliminary examine the relationship between CD80+ cells and CD8+ cytotoxic T lymphocytes. RESULTS: The expression of CD80 was downregulated in tumor tissues compared with normal tissues (p = 0.002). Immunohistochemistry analysis showed that 49 (39.8%) of 123 patients with gastric cancer demonstrated reduced CD80 expression, which was correlated with the tumor differentiation grade. CONCLUSION: Our data suggest that reduced CD80 expression independently predicts a poor prognosis in patients with gastric adenocarcinoma.
AIM: To study the expression and prognostic significance of CD80 in patients with gastric adenocarcinoma. Materials & methods: Real-time quantitative PCR, western blot and immunohistochemistry were performed to detect the expression of CD80 in gastric cancer tissues and matched adjacent normal tissues. Double immunohistochemical staining was performed to preliminary examine the relationship between CD80+ cells and CD8+ cytotoxic T lymphocytes. RESULTS: The expression of CD80 was downregulated in tumor tissues compared with normal tissues (p = 0.002). Immunohistochemistry analysis showed that 49 (39.8%) of 123 patients with gastric cancer demonstrated reduced CD80 expression, which was correlated with the tumor differentiation grade. CONCLUSION: Our data suggest that reduced CD80 expression independently predicts a poor prognosis in patients with gastric adenocarcinoma.
Entities:
Keywords:
CD80; cytotoxic T lymphocytes; gastric cancer; prognosis
Authors: Mark F Maurer; Katherine E Lewis; Joseph L Kuijper; Dan Ardourel; Chelsea J Gudgeon; Siddarth Chandrasekaran; Sherri L Mudri; Kayla N Kleist; Chris Navas; Martin F Wolfson; Mark W Rixon; Ryan Swanson; Stacey R Dillon; Steven D Levin; Yengo Raymond Kimbung; Masato Akutsu; Derek T Logan; Björn Walse; Kristine M Swiderek; Stanford L Peng Journal: Nat Commun Date: 2022-04-04 Impact factor: 17.694