Yun-Ui Bae1, Yongjin Kim2, Haekyung Lee3, Hyoungnae Kim3,4, Jin Seok Jeon3,4, Hyunjin Noh3,4, Dong Cheol Han3,4, Seongho Ryu1,5, Soon Hyo Kwon3,4. 1. Soonchunhyang Institute of Med-Bio Science, Soonchunhyang University, Cheonan, Chungchung nam do, Korea. 2. Department of Surgery, Soonchunhyang University, Cheonan, Chungchung nam do, Korea. 3. Department of Internal Medicine, Soonchunhyang University, Cheonan, Chungchung nam do, Korea. 4. Hyonam Kidney Laboratory, Soonchunhyang University Seoul Hospital, Seoul, Korea. 5. Laboratory of Pathology, Department of Medicine, Soonchunhyang University, Cheonan, Chungchung nam do, Korea.
Abstract
OBJECTIVE: Exosomal microRNAs (miRNAs) are potential biomarkers for obesity, in which they regulate biological processes. Bariatric surgery has health benefits for patients with obesity; however, the mechanisms of these benefits are not clear. This study attempted to identify the exosomal miRNA signature associated with obesity and how it changed after bariatric surgery. METHODS: Healthy volunteers (HVs) and nondiabetic patients with obesity were prospectively enrolled in the study. The study assessed the serum exosomal miRNA profiles of HVs and patients with obesity using RNA sequencing. To evaluate the effects of bariatric surgery, the study also analyzed exosomal miRNAs in patients 6 months after surgery. RESULTS: RNA sequencing revealed differential expression of 72 exosomal miRNAs in patients with obesity compared with HVs and differential expression of 41 miRNAs in post- versus presurgery blood. Among the differentially expressed miRNAs, the study identified nine surgery-responsive miRNAs that were highly expressed in patients before surgery compared with HVs. Biological pathway analysis of the nine miRNAs indicated that they are likely involved in WNT, neurotrophin, and insulin signaling; the insulin receptor signaling cascade; and focal adhesion. CONCLUSIONS: Patients with obesity have a distinct exosomal miRNA expression profile compared with HVs. In addition, weight loss after surgery alters the exosomal miRNA profile of patients with obesity.
OBJECTIVE: Exosomal microRNAs (miRNAs) are potential biomarkers for obesity, in which they regulate biological processes. Bariatric surgery has health benefits for patients with obesity; however, the mechanisms of these benefits are not clear. This study attempted to identify the exosomal miRNA signature associated with obesity and how it changed after bariatric surgery. METHODS: Healthy volunteers (HVs) and nondiabeticpatients with obesity were prospectively enrolled in the study. The study assessed the serum exosomal miRNA profiles of HVs and patients with obesity using RNA sequencing. To evaluate the effects of bariatric surgery, the study also analyzed exosomal miRNAs in patients 6 months after surgery. RESULTS: RNA sequencing revealed differential expression of 72 exosomal miRNAs in patients with obesity compared with HVs and differential expression of 41 miRNAs in post- versus presurgery blood. Among the differentially expressed miRNAs, the study identified nine surgery-responsive miRNAs that were highly expressed in patients before surgery compared with HVs. Biological pathway analysis of the nine miRNAs indicated that they are likely involved in WNT, neurotrophin, and insulin signaling; the insulin receptor signaling cascade; and focal adhesion. CONCLUSIONS:Patients with obesity have a distinct exosomal miRNA expression profile compared with HVs. In addition, weight loss after surgery alters the exosomal miRNA profile of patients with obesity.
Authors: Konstantinos Georgiou; Nikolay A Belev; Tilemachos Koutouratsas; Hector Katifelis; Maria Gazouli Journal: World J Gastrointest Pathophysiol Date: 2022-05-22
Authors: G Catanzaro; T Filardi; C Sabato; A Vacca; S Migliaccio; S Morano; E Ferretti Journal: J Endocrinol Invest Date: 2020-10-28 Impact factor: 4.256