Dughyun Choi1, Sewon Kim2,3, Jeyoung Woo2, Haekyung Lee4, Hyoungnae Kim4, Jin Seok Jeon4, Hyunjin Noh4, Dong Cheol Han4, Sang Hyun Kim5, Ho Chan Cho6, Jong-Soo Choi7, Yun-Ui Bae6, Soon Hyo Kwon4. 1. Division of Endocrinology and Metabolism, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, Republic of Korea. 2. Macrogen Inc., Seoul, Republic of Korea. 3. ROKIT Genomics, Seoul, Republic of Korea. 4. Division of Nephrology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, Republic of Korea. 5. Department of Surgery, Soonchunhyang University Seoul Hospital, Seoul, Republic of Korea. 6. Department of Clinical Endocrinology, Keimyung University School of Medicine, Daegu, Republic of Korea. 7. Research Institute, Biosolyx Co. Ltd., Daegu, Republic of Korea.
Abstract
INTRODUCTION: Various kidney diseases reportedly show different urinary extracellular vesicle (EV) RNA profiles. Although obesity is one of the main causes of chronic kidney disease, the expression pattern of urinary EV RNA in obesity is uncertain. Our aim was to sequence the small RNA profiles of urinary EVs in obese patients before and after weight reduction and compare them to those of healthy volunteers (HVs). METHODS: We recruited age-sex-matched obese patients and HVs. The small RNA profiles of urinary EVs were analyzed using RNA sequencing. To evaluate the effect of weight reduction, small RNA profiles of urinary EVs 6 months after bariatric surgery were also analyzed. RESULTS: The proportion of urinary EVs transfer RNA and microRNA of obese patients differed from that of HVs. Obese patients showed differential expression of 1,343 small RNAs in urinary EVs compared to HVs (fold change ≥2 and p value <0.05). Among those, 61 small RNAs were upregulated in obese patients and downregulated after weight reduction, whereas 167 small RNAs were downregulated in obese patients and upregulated after weight reduction. RNA sequencing revealed the correlation between the specific urinary EV small RNAs and clinical parameters including body weight, low-density lipoprotein cholesterol, triglyceride, high-density lipoprotein cholesterol, serum glucose, estimated glomerular filtration rate, and albuminuria. CONCLUSION: Obese patients showed distinct urinary EV small RNA profiles compared to HVs. Weight reduction altered urinary EV small-RNA profiles in obese patients.
INTRODUCTION: Various kidney diseases reportedly show different urinary extracellular vesicle (EV) RNA profiles. Although obesity is one of the main causes of chronic kidney disease, the expression pattern of urinary EV RNA in obesity is uncertain. Our aim was to sequence the small RNA profiles of urinary EVs in obese patients before and after weight reduction and compare them to those of healthy volunteers (HVs). METHODS: We recruited age-sex-matched obese patients and HVs. The small RNA profiles of urinary EVs were analyzed using RNA sequencing. To evaluate the effect of weight reduction, small RNA profiles of urinary EVs 6 months after bariatric surgery were also analyzed. RESULTS: The proportion of urinary EVs transfer RNA and microRNA of obese patients differed from that of HVs. Obese patients showed differential expression of 1,343 small RNAs in urinary EVs compared to HVs (fold change ≥2 and p value <0.05). Among those, 61 small RNAs were upregulated in obese patients and downregulated after weight reduction, whereas 167 small RNAs were downregulated in obese patients and upregulated after weight reduction. RNA sequencing revealed the correlation between the specific urinary EV small RNAs and clinical parameters including body weight, low-density lipoprotein cholesterol, triglyceride, high-density lipoprotein cholesterol, serum glucose, estimated glomerular filtration rate, and albuminuria. CONCLUSION: Obese patients showed distinct urinary EV small RNA profiles compared to HVs. Weight reduction altered urinary EV small-RNA profiles in obese patients.
Authors: Hina Y Bhutta; Neetu Rajpal; Wendy White; Johannes M Freudenberg; Yaping Liu; James Way; Deepak Rajpal; David C Cooper; Andrew Young; Ali Tavakkoli; Lihong Chen Journal: PLoS One Date: 2015-03-23 Impact factor: 3.240
Authors: Paula M Godoy; Nirav R Bhakta; Andrea J Barczak; Hakan Cakmak; Susan Fisher; Tippi C MacKenzie; Tushar Patel; Richard W Price; James F Smith; Prescott G Woodruff; David J Erle Journal: Cell Rep Date: 2018-10-30 Impact factor: 9.423