Literature DB >> 30623494

Disabling of nephrogenesis in porcine embryos via CRISPR/Cas9-mediated SIX1 and SIX4 gene targeting.

Junzheng Wang1, Manling Liu1, Lihua Zhao1, Yanru Li1,2, Manling Zhang1,3, Yong Jin1,4, Qiang Xiong1, Xiaorui Liu1, Lining Zhang1, Haibin Jiang1, Qiaoyu Chen1, Chenyu Wang1, Zhihuan You1, Haiyuan Yang1,3, Changchun Cao4, Yifan Dai1,2,3, Rongfeng Li1,2,3.   

Abstract

SIX1 and SIX4 genes play critical roles in kidney development. We evaluated the effect of these genes on pig kidney development by generating SIX1-/- and SIX1-/- /SIX4-/- pig foetuses using CRISPR/Cas9 and somatic cell nuclear transfer. We obtained 3 SIX1-/- foetuses and 16 SIX1-/- /SIX4-/- foetuses at different developmental stages. The SIX1-/- foetuses showed a migration block of the left kidney and a smaller size for both kidneys. The ureteric bud failed to form the normal branching and collecting system. Abnormal expressions of kidney development-related genes (downregulation of PAX2, PAX8, and BMP4 and upregulation of EYA1 and SALL1) were also observed in SIX1-/- foetal kidneys and confirmed in vitro in porcine kidney epithelial cells (PK15) following SIX1 gene deletion. The SIX1-/- /SIX4-/- foetuses exhibited more severe phenotypes, with most foetuses showing retarded development at early stages of gestation. The kidney developed only to the initial stage of metanephros formation. These results demonstrated that SIX1 and SIX4 are key genes for porcine metanephros development. The creation of kidney-deficient porcine foetuses provides a platform for generating human kidneys inside pigs using blastocyst complementation.
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Entities:  

Keywords:  zzm321990SIX1zzm321990; zzm321990SIX4zzm321990; CRISPR/Cas9; blastocyst complementation; kidney development; pig

Mesh：

Substances：

Year:  2019        PMID： 30623494     DOI： 10.1111/xen.12484

Source DB:  PubMed          Journal:  Xenotransplantation        ISSN： 0908-665X            Impact factor:   3.907


  6 in total

1.  SIX1 Regulates Aberrant Endometrial Epithelial Cell Differentiation and Cancer Latency Following Developmental Estrogenic Chemical Exposure.

Authors:  Alisa A Suen; Wendy N Jefferson; Charles E Wood; Carmen J Williams
Journal:  Mol Cancer Res       Date:  2019-10-09       Impact factor: 5.852

2.  Mutations of the Transcriptional Corepressor ZMYM2 Cause Syndromic Urinary Tract Malformations.

Authors:  Dervla M Connaughton; Rufeng Dai; Danielle J Owen; Jonathan Marquez; Nina Mann; Adda L Graham-Paquin; Makiko Nakayama; Etienne Coyaud; Estelle M N Laurent; Jonathan R St-Germain; Lot Snijders Blok; Arianna Vino; Verena Klämbt; Konstantin Deutsch; Chen-Han Wilfred Wu; Caroline M Kolvenbach; Franziska Kause; Isabel Ottlewski; Ronen Schneider; Thomas M Kitzler; Amar J Majmundar; Florian Buerger; Ana C Onuchic-Whitford; Mao Youying; Amy Kolb; Daanya Salmanullah; Evan Chen; Amelie T van der Ven; Jia Rao; Hadas Ityel; Steve Seltzsam; Johanna M Rieke; Jing Chen; Asaf Vivante; Daw-Yang Hwang; Stefan Kohl; Gabriel C Dworschak; Tobias Hermle; Mariëlle Alders; Tobias Bartolomaeus; Stuart B Bauer; Michelle A Baum; Eva H Brilstra; Thomas D Challman; Jacob Zyskind; Carrie E Costin; Katrina M Dipple; Floor A Duijkers; Marcia Ferguson; David R Fitzpatrick; Roger Fick; Ian A Glass; Peter J Hulick; Antonie D Kline; Ilona Krey; Selvin Kumar; Weining Lu; Elysa J Marco; Ingrid M Wentzensen; Heather C Mefford; Konrad Platzer; Inna S Povolotskaya; Juliann M Savatt; Natalia V Shcherbakova; Prabha Senguttuvan; Audrey E Squire; Deborah R Stein; Isabelle Thiffault; Victoria Y Voinova; Michael J G Somers; Michael A Ferguson; Avram Z Traum; Ghaleb H Daouk; Ankana Daga; Nancy M Rodig; Paulien A Terhal; Ellen van Binsbergen; Loai A Eid; Velibor Tasic; Hila Milo Rasouly; Tze Y Lim; Dina F Ahram; Ali G Gharavi; Heiko M Reutter; Heidi L Rehm; Daniel G MacArthur; Monkol Lek; Kristen M Laricchia; Richard P Lifton; Hong Xu; Shrikant M Mane; Simone Sanna-Cherchi; Andrew D Sharrocks; Brian Raught; Simon E Fisher; Maxime Bouchard; Mustafa K Khokha; Shirlee Shril; Friedhelm Hildebrandt
Journal:  Am J Hum Genet       Date:  2020-09-04       Impact factor: 11.025

Review 3.  Interspecies Organogenesis for Human Transplantation.

Authors:  Andrew T Crane; Rajagopal N Aravalli; Atsushi Asakura; Andrew W Grande; Venkatramana D Krishna; Daniel F Carlson; Maxim C-J Cheeran; Georgette Danczyk; James R Dutton; Perry B Hackett; Wei-Shou Hu; Ling Li; Wei-Cheng Lu; Zachary D Miller; Timothy D O'Brien; Angela Panoskaltsis-Mortari; Ann M Parr; Clairice Pearce; Mercedes Ruiz-Estevez; Maple Shiao; Christopher J Sipe; Nikolas G Toman; Joseph Voth; Hui Xie; Clifford J Steer; Walter C Low
Journal:  Cell Transplant       Date:  2019-08-19       Impact factor: 4.064

Review 4.  Improvements in Gene Editing Technology Boost Its Applications in Livestock.

Authors:  Iuri Viotti Perisse; Zhiqiang Fan; Galina N Singina; Kenneth L White; Irina A Polejaeva
Journal:  Front Genet       Date:  2021-01-08       Impact factor: 4.599

5.  CRISPR/Cas9-Mediated α-ENaC Knockout in a Murine Pancreatic β-Cell Line.

Authors:  Xue Zhang; Lihua Zhao; Runbing Jin; Min Li; Mei-Shuang Li; Rongfeng Li; Xiubin Liang
Journal:  Front Genet       Date:  2021-04-01       Impact factor: 4.599

Review 6.  In Vivo Generation of Organs by Blastocyst Complementation: Advances and Challenges.

Authors:  Konstantina-Maria Founta; Costis Papanayotou
Journal:  Int J Stem Cells       Date:  2022-05-30       Impact factor: 3.011
  6 in total

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