Xiao-Wei Gao1,2, Xiao-Min Zhang3, Hai-Yan Liu2, Shan-Shan Wang2, Hua-Jiang Dong4. 1. Tianjin Medical University Eye Institute and Tianjin Medical University School of Optometry and Ophthalmology, Tianjin Medical University Eye Hospital, Tianjin, 300384, China. 2. Department of Otolaryngology-Head and Neck Surgery, Second Hospital of Tianjin Medical University, Tianjin, 300211, China. 3. Tianjin Medical University Eye Institute and Tianjin Medical University School of Optometry and Ophthalmology, Tianjin Medical University Eye Hospital, Tianjin, 300384, China. xiaomzh@126.com. 4. State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University, Tianjin, 300072, China.
Abstract
PURPOSE: The mechanisms of naso-ocular interaction in allergic rhinoconjunctivitis are not well understood. Neurogenic inflammation affects both eyes and nose via the same neurogenic factors. The purpose of this study was to investigate the effects of neurogenic inflammation on conjunctival inflammation following nasal allergen provocation. METHODS: Sensitized rats were exposed to ovalbumin (OVA) via the nose. Parts of the nasal mucosa and conjunctivae were sliced and used for hematoxylin-eosin staining, immunohistochemical analysis, western blotting, and real-time polymerase chain reaction. The slides were observed under a light microscope, and the acquired images were analyzed. The levels of substance P (SP), vasoactive intestinal peptide (VIP), and nerve growth factor (NGF) were detected. RESULTS: The levels of SP, VIP, and NGF were increased in both nasal mucosa and conjunctivae 1 h and 24 h after OVA administration (p < 0.05). Higher levels of SP, VIP, and NGF expression were observed in the nasal mucosa and conjunctivae 24 h after OVA administration (p < 0.05). Following damage of the nasal sensory nerves by capsaicin, the protein and mRNA levels of SP, VIP, and NGF were reduced. CONCLUSION: In conclusion, the increased levels of VIP, SP, and NGF might be responsible for the ocular reaction following nasal challenge with allergen in rats.
PURPOSE: The mechanisms of naso-ocular interaction in allergic rhinoconjunctivitis are not well understood. Neurogenic inflammation affects both eyes and nose via the same neurogenic factors. The purpose of this study was to investigate the effects of neurogenic inflammation on conjunctival inflammation following nasal allergen provocation. METHODS: Sensitized rats were exposed to ovalbumin (OVA) via the nose. Parts of the nasal mucosa and conjunctivae were sliced and used for hematoxylin-eosin staining, immunohistochemical analysis, western blotting, and real-time polymerase chain reaction. The slides were observed under a light microscope, and the acquired images were analyzed. The levels of substance P (SP), vasoactive intestinal peptide (VIP), and nerve growth factor (NGF) were detected. RESULTS: The levels of SP, VIP, and NGF were increased in both nasal mucosa and conjunctivae 1 h and 24 h after OVA administration (p < 0.05). Higher levels of SP, VIP, and NGF expression were observed in the nasal mucosa and conjunctivae 24 h after OVA administration (p < 0.05). Following damage of the nasal sensory nerves by capsaicin, the protein and mRNA levels of SP, VIP, and NGF were reduced. CONCLUSION: In conclusion, the increased levels of VIP, SP, and NGF might be responsible for the ocular reaction following nasal challenge with allergen in rats.
Authors: I Callebaut; L Spielberg; V Hox; S Bobic; M Jorissen; I Stalmans; G Scadding; J L Ceuppens; P W Hellings Journal: Allergy Date: 2010-04-23 Impact factor: 13.146
Authors: S G O Johansson; Thomas Bieber; Ronald Dahl; Peter S Friedmann; Bobby Q Lanier; Richard F Lockey; Cassim Motala; Jose A Ortega Martell; Thomas A E Platts-Mills; Johannes Ring; Frank Thien; Paul Van Cauwenberge; Hywel C Williams Journal: J Allergy Clin Immunol Date: 2004-05 Impact factor: 10.793