Literature DB >> 3060418

Production and purification of Escherichia coli hemolysin.

P J Asnani1, N Bhatnagar, S Bhandari.   

Abstract

Eight strains of Escherichia coli, isolated from patients with a urinary tract infection were investigated for production of hemolysin. Six of these produced hemolysin and one revealed maximum hemolytic activity. Three urinary and two faecal isolates were positive for mannose-resistant hemagglutination. One isolate positive for hemagglutination and giving maximum hemolytic activity was then used. Hemolysin was present in the supernatant broth and the medium of choice to obtain the optimum yield was the alkaline meat extract broth followed by brain heart infusion broth. The highest yield appeared in the exponential phase of growth. Hemolysin is a heat-labile protein, being produced optimally at pH 8. A three-stage procedure was the best method for its purification.

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Year:  1988        PMID： 3060418     DOI： 10.1007/bf02925850

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  16 in total

1.  Properties of strains of Escherichia coli isolated from a variety of sources.

Authors:  E M Cooke; S P Ewins
Journal:  J Med Microbiol       Date:  1975-02       Impact factor: 2.472

2.  On Bacillus coli Infections of the Urinary Tract especially in relation to Haemolytic Organisms.

Authors:  L S Dudgeon; E Wordley; F Bawtree
Journal:  J Hyg (Lond)       Date:  1922-11

3.  Haemolysin and necrotoxin production by Escherichia coli.

Authors:  R C Pande; S C Gupta; V S Rajvanshi; T N Mehrotra
Journal:  Indian J Med Res       Date:  1974-10       Impact factor: 2.375

4.  The kinetics of erythrocyte lysis by Escherichia coli haemolysin.

Authors:  R P Rennie; J H Freer; J P Arbuthnott
Journal:  J Med Microbiol       Date:  1974-05       Impact factor: 2.472

5.  Purification and kinetic studies of the hemolysin from Escherichia coli.

Authors:  P Zwadyk; I S Snyder
Journal:  Can J Microbiol       Date:  1971-06       Impact factor: 2.419

6.  Studies on hemolytic Escherichia coli of O-139. A certain factor essential for hemolysin production.

Authors:  Y Inukai; H Kodama
Journal:  Jpn J Vet Res       Date:  1965-09       Impact factor: 0.649

7.  Pathogenesis of edema disease in swine: pathologic effects of hemolysin, autolysate, and endotoxin of Escherichia coli (O141).

Authors:  H J Kurtz; E C Short
Journal:  Am J Vet Res       Date:  1976-01       Impact factor: 1.156

8.  Haemolysin and necrotoxin production of Escherichia coli with special reference to serotypes causing urinary tract infections.

Authors:  A Prabhu; A D Mondkar; M S Nadkarni
Journal:  Indian J Pathol Microbiol       Date:  1981-01       Impact factor: 0.740

9.  Association of hemolysin production, hemagglutination of human erythrocytes, and virulence for chicken embryos of extraintestinal Escherichia coli isolates.

Authors:  B H Minshew; J Jorgensen; G W Counts; S Falkow
Journal:  Infect Immun       Date:  1978-04       Impact factor: 3.441

10.  Cytotoxic activity of partially purified Escherichia coli alpha haemolysin.

Authors:  S J Cavalieri; I S Snyder
Journal:  J Med Microbiol       Date:  1982-02       Impact factor: 2.472
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  3 in total

1.  Partial purification of alpha-hemolysin in Escherichia coli.

Authors:  F Nistiar; V Janigová; J Durovicová; H Puzová
Journal:  Folia Microbiol (Praha)       Date:  1991       Impact factor: 2.099

Review 2.  Virulence factors in Escherichia coli urinary tract infection.

Authors:  J R Johnson
Journal:  Clin Microbiol Rev       Date:  1991-01       Impact factor: 26.132

3.  Partial characterization of Streptococcus suis type 2 hemolysin.

Authors:  I Feder; M M Chengappa; B Fenwick; M Rider; J Staats
Journal:  J Clin Microbiol       Date:  1994-05       Impact factor: 5.948
  3 in total

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