Literature DB >> 1822842

Partial purification of alpha-hemolysin in Escherichia coli.

F Nistiar1, V Janigová, J Durovicová, H Puzová.   

Abstract

The present paper describes isolation and purification of alpha-hemolysin of Escherichia coli. The optimum production medium was found to be the Todd-Hewitt broth. Out of thirteen fractions obtained after separation on Sephadex G-200, two fractions possessed the highest relative specific activity.

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Year:  1991        PMID： 1822842     DOI： 10.1007/BF02814516

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  31 in total

1.  Production and purification of Escherichia coli hemolysin.

Authors:  P J Asnani; N Bhatnagar; S Bhandari
Journal:  Folia Microbiol (Praha)       Date:  1988       Impact factor: 2.099

2.  Role of alpha-hemolysin for the in vitro phagocytosis and intracellular killing of Escherichia coli.

Authors:  O V Gadeberg; J Hacker; I Orskov
Journal:  Zentralbl Bakteriol       Date:  1989-07

3.  Characterisation of HlyC and mechanism of activation and secretion of haemolysin from E. coli 2001.

Authors:  J M Nicaud; N Mackman; L Gray; I B Holland
Journal:  FEBS Lett       Date:  1985-08-05       Impact factor: 4.124

4.  In vitro cytotoxic effect of alpha-hemolytic Escherichia coli on human blood granulocytes.

Authors:  O V Gadeberg; I Orskov
Journal:  Infect Immun       Date:  1984-07       Impact factor: 3.441

5.  Identification with monoclonal antibodies of hemolysin produced by clinical isolates of Escherichia coli.

Authors:  F Hugo; M Arvand; J Reichwein; N Mackman; I B Holland; S Bhakdi
Journal:  J Clin Microbiol       Date:  1987-01       Impact factor: 5.948

6.  Chemical and immunological analysis of the complex structure of Escherichia coli alpha-hemolysin.

Authors:  G A Bohach; I S Snyder
Journal:  J Bacteriol       Date:  1985-12       Impact factor: 3.490

7.  Escherichia coli hemolysin may damage target cell membranes by generating transmembrane pores.

Authors:  S Bhakdi; N Mackman; J M Nicaud; I B Holland
Journal:  Infect Immun       Date:  1986-04       Impact factor: 3.441

8.  Cytotoxic activity of partially purified Escherichia coli alpha haemolysin.

Authors:  S J Cavalieri; I S Snyder
Journal:  J Med Microbiol       Date:  1982-02       Impact factor: 2.472

9.  Molecular cloning and physical characterization of a chromosomal hemolysin from Escherichia coli.

Authors:  R A Welch; R Hull; S Falkow
Journal:  Infect Immun       Date:  1983-10       Impact factor: 3.441

10.  Effect of Escherichia coli alpha-hemolysin on human peripheral leukocyte function in vitro.

Authors:  S J Cavalieri; I S Snyder
Journal:  Infect Immun       Date:  1982-09       Impact factor: 3.441
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