Literature DB >> 30601885

Efficacy of Transplantation of Combination of Noncultured Dermal and Epidermal Cell Suspension vs Epidermal Cell Suspension Alone in Vitiligo: A Randomized Clinical Trial.

Vishal Thakur1, Sheetanshu Kumar1, Muthu Sendhil Kumaran1, Hitaishi Kaushik1, Niharika Srivastava1, Davinder Parsad1.   

Abstract

Importance: Surgical interventions, notably noncultured epidermal suspension (NCES), are the next line of treatment in patients with vitiligo who fail to respond to medical therapy. Noncultured epidermal suspension is usually performed in patients with vitiligo with duration of clinical stability (DS) of 12 months or longer because DS is a vital parameter in determining outcome of NCES. In this pilot study, we planned to assess the efficacy of a novel combination of noncultured epidermal cell suspension and noncultured dermal cell suspension (NCES and NDCS) in patients with vitiligo with shorter DS (3-6 months). Objective: To compare the efficacy of transplantation of NCES and NDCS vs NCES alone in patients with vitiligo with DS of 3 to 6 months. Design, Setting, and Participants: A single-center randomized clinical trial including 40 patients with focal, segmental, or generalized vitiligo with DS of 3 to 6 months or more than 12 months was carried out. Based on DS, 2 groups including 20 patients each were recruited (DS in group 1, 3 to 6 months; DS in group 2, more than 12 months). Each group was further randomized into 2 subgroups, A and B. Intervention: Patients in subgroups 1A and 2A underwent NCES alone, whereas patients in subgroups 1B and 2B underwent NCES and NDCS. Main Outcomes and Measures: Extent of repigmentation, color match, and pattern of repigmentation at 24 weeks.
Results: Of the 40 study participants, mean (SD) age was 24.9 (4.0) years and 24 (60%) were women; in group 1 with DS for 3 to 6 months, more than 75% repigmentation at 24 weeks was observed in all 10 patients in subgroup 1B (NCES and NDCS) compared with 3 of 10 patients in subgroup 1A (NCES) (100% vs 30%, P = .003). In group 2 (DS > 12 months), the same was observed in 6 of 10 patients in subgroup 2A and 7 of 10 patients in subgroup 2B (NCES) (60% vs 70%, P > .99). The 2 groups and subgroups did not show any significant differences with respect to color matching and pattern of repigmentation. Conclusions and Relevance: Combination of NCES and NDCS resulted in excellent response in patients with vitiligo with shorter duration of clinical stability compared with NCES alone. This combination may be used early in the course of stable vitiligo without waiting for a period of 12 months or more since last clinical activity. Trial Registration: ClinicalTrials.gov identifier: NCT03013049.

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Year:  2019        PMID: 30601885      PMCID: PMC6439545          DOI: 10.1001/jamadermatol.2018.4919

Source DB:  PubMed          Journal:  JAMA Dermatol        ISSN: 2168-6068            Impact factor:   10.282


  18 in total

1.  Mesenchymal stem cells induce apoptosis of activated T cells.

Authors:  J Plumas; L Chaperot; M-J Richard; J-P Molens; J-C Bensa; M-C Favrot
Journal:  Leukemia       Date:  2005-09       Impact factor: 11.528

Review 2.  Immunomodulatory properties of mesenchymal stromal cells.

Authors:  Alma J Nauta; Willem E Fibbe
Journal:  Blood       Date:  2007-07-30       Impact factor: 22.113

3.  Autoimmune destruction of skin melanocytes by perilesional T cells from vitiligo patients.

Authors:  Jasper G van den Boorn; Debby Konijnenberg; Trees A M Dellemijn; J P Wietze van der Veen; Jan D Bos; Cornelis J M Melief; Florry A Vyth-Dreese; Rosalie M Luiten
Journal:  J Invest Dermatol       Date:  2009-02-26       Impact factor: 8.551

4.  Noncultured extracted hair follicle outer root sheath cell suspension for transplantation in vitiligo.

Authors:  S Mohanty; A Kumar; J Dhawan; V Sreenivas; S Gupta
Journal:  Br J Dermatol       Date:  2011-06       Impact factor: 9.302

5.  Autologous grafting with noncultured melanocytes: a simplified method for treatment of depigmented lesions.

Authors:  Y Gauthier; J E Surleve-Bazeille
Journal:  J Am Acad Dermatol       Date:  1992-02       Impact factor: 11.527

6.  Comparison between autologous noncultured epidermal cell suspension and suction blister epidermal grafting in stable vitiligo: a randomized study.

Authors:  A Budania; D Parsad; A J Kanwar; S Dogra
Journal:  Br J Dermatol       Date:  2012-12       Impact factor: 9.302

7.  Study of clinical, biochemical and immunological factors determining stability of disease in patients with generalized vitiligo undergoing melanocyte transplantation.

Authors:  A Rao; S Gupta; A K Dinda; A Sharma; V K Sharma; G Kumar; D K Mitra; C K Prashant; G Singh
Journal:  Br J Dermatol       Date:  2012-06       Impact factor: 9.302

8.  Combination of Follicular and Epidermal Cell Suspension as a Novel Surgical Approach in Difficult-to-Treat Vitiligo: A Randomized Clinical Trial.

Authors:  Muhammed Razmi T; Ravinder Kumar; Seema Rani; Sendhil M Kumaran; Sushma Tanwar; Davinder Parsad
Journal:  JAMA Dermatol       Date:  2018-03-01       Impact factor: 10.282

9.  Immunopolarization of CD4+ and CD8+ T cells to Type-1-like is associated with melanocyte loss in human vitiligo.

Authors:  Anna Wańkowicz-Kalińska; René M J G J van den Wijngaard; Bert J Tigges; Wiete Westerhof; Graham S Ogg; Vincenzo Cerundolo; Walter J Storkus; Pranab K Das
Journal:  Lab Invest       Date:  2003-05       Impact factor: 5.662

10.  Dermal mesenchymal stem cells (DMSCs) inhibit skin-homing CD8+ T cell activity, a determining factor of vitiligo patients' autologous melanocytes transplantation efficiency.

Authors:  Miao-ni Zhou; Zhi-qing Zhang; Ji-long Wu; Fu-quan Lin; Li-fang Fu; Sui-qan Wang; Cui-ping Guan; Hong-lin Wang; Aie Xu
Journal:  PLoS One       Date:  2013-04-05       Impact factor: 3.240

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  3 in total

Review 1.  Regenerative Medicine of Epithelia: Lessons From the Past and Future Goals.

Authors:  Eleonora Maurizi; Davide Adamo; Federica Maria Magrelli; Giulia Galaverni; Eustachio Attico; Alessia Merra; Maria Benedetta Rizzarda Maffezzoni; Lorena Losi; Vincenzo Giuseppe Genna; Virginia Sceberras; Graziella Pellegrini
Journal:  Front Bioeng Biotechnol       Date:  2021-03-25

Review 2.  Vitiligo: An Autoimmune Skin Disease and its Immunomodulatory Therapeutic Intervention.

Authors:  Wei-Ling Chang; Woan-Ruoh Lee; Yung-Che Kuo; Yen-Hua Huang
Journal:  Front Cell Dev Biol       Date:  2021-12-14

3.  Donor to recipient ratios in the surgical treatment of vitiligo and piebaldism: a systematic review.

Authors:  V S Narayan; L L C van den Bol; N van Geel; M W Bekkenk; R M Luiten; A Wolkerstorfer
Journal:  J Eur Acad Dermatol Venereol       Date:  2021-02-12       Impact factor: 6.166

  3 in total

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